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The Metabolites of Lactobacillus fermentum F-B9-1 Relieved Dextran Sulfate Sodium-Induced Experimental Ulcerative Colitis in Mice

Because of the increased incidence and prevalence, ulcerative colitis (UC) has become a global health issue in the world. Current therapies for UC are not totally effective which result in persistent and recurrent symptom of many patients. Lactobacillus with anti-inflammatory effects might be benefi...

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Autores principales: Su, Le, Ma, Feifan, An, Zaiyong, Ji, Xiuyu, Zhang, Ping, Yue, Qiulin, Zhao, Chen, Sun, Xin, Li, Kunlun, Li, Baojun, Liu, Xinli, Zhao, Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9096258/
https://www.ncbi.nlm.nih.gov/pubmed/35572623
http://dx.doi.org/10.3389/fmicb.2022.865925
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author Su, Le
Ma, Feifan
An, Zaiyong
Ji, Xiuyu
Zhang, Ping
Yue, Qiulin
Zhao, Chen
Sun, Xin
Li, Kunlun
Li, Baojun
Liu, Xinli
Zhao, Lin
author_facet Su, Le
Ma, Feifan
An, Zaiyong
Ji, Xiuyu
Zhang, Ping
Yue, Qiulin
Zhao, Chen
Sun, Xin
Li, Kunlun
Li, Baojun
Liu, Xinli
Zhao, Lin
author_sort Su, Le
collection PubMed
description Because of the increased incidence and prevalence, ulcerative colitis (UC) has become a global health issue in the world. Current therapies for UC are not totally effective which result in persistent and recurrent symptom of many patients. Lactobacillus with anti-inflammatory effects might be beneficial to the prevention or treatment for UC. Here, we examined the ameliorative effects of the metabolites of Lactobacillus fermentum F-B9-1 (MLF) in Caco-2 cells and dextran sodium sulfate (DSS)-induced UC model mice. MLF displayed intestinal barrier-protective activities in Caco-2 cells by increasing the expression of Occludin and ZO-1. They also showed anti-inflammatory potential in interleukin (IL)-1β and IL-6. In order to further examine the in vivo anti-inflammatory effect of MLF, the MLF was gavaged in the DSS-induced UC model mice. The intragastric administration of MLF effectively alleviated colitis symptoms of weight loss, diarrhea, colon shortening, and histopathological scores, protected intestinal barrier function by increasing Occludin and ZO-1, and attenuated colonic and systemic inflammation by suppressing production of IL-1β and IL-6. Finally, the use of MLF remodeled the diversity of the gut microbiota and increased the number of beneficial microorganisms. Overall, the results demonstrated that MLF relieved DSS-induced UC in mice. And MLF might be an effective therapy method to UC in the clinic in the future.
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spelling pubmed-90962582022-05-13 The Metabolites of Lactobacillus fermentum F-B9-1 Relieved Dextran Sulfate Sodium-Induced Experimental Ulcerative Colitis in Mice Su, Le Ma, Feifan An, Zaiyong Ji, Xiuyu Zhang, Ping Yue, Qiulin Zhao, Chen Sun, Xin Li, Kunlun Li, Baojun Liu, Xinli Zhao, Lin Front Microbiol Microbiology Because of the increased incidence and prevalence, ulcerative colitis (UC) has become a global health issue in the world. Current therapies for UC are not totally effective which result in persistent and recurrent symptom of many patients. Lactobacillus with anti-inflammatory effects might be beneficial to the prevention or treatment for UC. Here, we examined the ameliorative effects of the metabolites of Lactobacillus fermentum F-B9-1 (MLF) in Caco-2 cells and dextran sodium sulfate (DSS)-induced UC model mice. MLF displayed intestinal barrier-protective activities in Caco-2 cells by increasing the expression of Occludin and ZO-1. They also showed anti-inflammatory potential in interleukin (IL)-1β and IL-6. In order to further examine the in vivo anti-inflammatory effect of MLF, the MLF was gavaged in the DSS-induced UC model mice. The intragastric administration of MLF effectively alleviated colitis symptoms of weight loss, diarrhea, colon shortening, and histopathological scores, protected intestinal barrier function by increasing Occludin and ZO-1, and attenuated colonic and systemic inflammation by suppressing production of IL-1β and IL-6. Finally, the use of MLF remodeled the diversity of the gut microbiota and increased the number of beneficial microorganisms. Overall, the results demonstrated that MLF relieved DSS-induced UC in mice. And MLF might be an effective therapy method to UC in the clinic in the future. Frontiers Media S.A. 2022-04-28 /pmc/articles/PMC9096258/ /pubmed/35572623 http://dx.doi.org/10.3389/fmicb.2022.865925 Text en Copyright © 2022 Su, Ma, An, Ji, Zhang, Yue, Zhao, Sun, Li, Li, Liu and Zhao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Su, Le
Ma, Feifan
An, Zaiyong
Ji, Xiuyu
Zhang, Ping
Yue, Qiulin
Zhao, Chen
Sun, Xin
Li, Kunlun
Li, Baojun
Liu, Xinli
Zhao, Lin
The Metabolites of Lactobacillus fermentum F-B9-1 Relieved Dextran Sulfate Sodium-Induced Experimental Ulcerative Colitis in Mice
title The Metabolites of Lactobacillus fermentum F-B9-1 Relieved Dextran Sulfate Sodium-Induced Experimental Ulcerative Colitis in Mice
title_full The Metabolites of Lactobacillus fermentum F-B9-1 Relieved Dextran Sulfate Sodium-Induced Experimental Ulcerative Colitis in Mice
title_fullStr The Metabolites of Lactobacillus fermentum F-B9-1 Relieved Dextran Sulfate Sodium-Induced Experimental Ulcerative Colitis in Mice
title_full_unstemmed The Metabolites of Lactobacillus fermentum F-B9-1 Relieved Dextran Sulfate Sodium-Induced Experimental Ulcerative Colitis in Mice
title_short The Metabolites of Lactobacillus fermentum F-B9-1 Relieved Dextran Sulfate Sodium-Induced Experimental Ulcerative Colitis in Mice
title_sort metabolites of lactobacillus fermentum f-b9-1 relieved dextran sulfate sodium-induced experimental ulcerative colitis in mice
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9096258/
https://www.ncbi.nlm.nih.gov/pubmed/35572623
http://dx.doi.org/10.3389/fmicb.2022.865925
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