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Heterogeneous microenvironment analysis to explore the potential regulatory role of endothelial-mesenchymal transition in idiopathic pulmonary fibrosis

BACKGROUND: Idiopathic pulmonary fibrosis (IPF) is a chronic and progressive interstitial lung disease mainly caused by excessive proliferation of fibroblasts and activation of myofibroblasts. The cellular microenvironment is mainly composed of different types of cellular components and extracellula...

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Autores principales: Xu, Yinning, Hu, Xiaoping, Zhang, Yiwen, Pan, Zongfu, Sun, Zhiyong, Huang, Zhongjie, Zheng, Shuilian, Pan, Hansheng, Zou, Xiaozhou, Huang, Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AME Publishing Company 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9096361/
https://www.ncbi.nlm.nih.gov/pubmed/35571445
http://dx.doi.org/10.21037/atm-22-1438
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author Xu, Yinning
Hu, Xiaoping
Zhang, Yiwen
Pan, Zongfu
Sun, Zhiyong
Huang, Zhongjie
Zheng, Shuilian
Pan, Hansheng
Zou, Xiaozhou
Huang, Ping
author_facet Xu, Yinning
Hu, Xiaoping
Zhang, Yiwen
Pan, Zongfu
Sun, Zhiyong
Huang, Zhongjie
Zheng, Shuilian
Pan, Hansheng
Zou, Xiaozhou
Huang, Ping
author_sort Xu, Yinning
collection PubMed
description BACKGROUND: Idiopathic pulmonary fibrosis (IPF) is a chronic and progressive interstitial lung disease mainly caused by excessive proliferation of fibroblasts and activation of myofibroblasts. The cellular microenvironment is mainly composed of different types of cellular components and extracellular matrix (ECM), whose changes directly affect cellular heterogeneity, resulting in immensely complex cellular interactions. However, microenvironment study is mainly focused on the pathological process of tumors, and the microenvironment changes during IPF development remain unclear. METHODS: The current study intends to employ IPF-related single-cell sequencing and gene expression profile data to analyze the scores of different cell clusters in the IPF microenvironment, and exploit the underlying interaction between cells to illustrate the fundamental mechanism causing IPF. RESULTS: Our analysis revealed that the amount of endothelial cells was obviously decreased, and the amount of fibroblasts and myofibroblasts was increased during the development of IPF, suggesting a possible endothelial-mesenchymal transition (EndMT) process. Furthermore, we found that the hub genes obtained through IPF-related gene expression profile analysis may play a regulative role in the number and function of endothelial cells and fibroblasts/myofibroblasts during IPF. CONCLUSIONS: Our research represents a valuable analysis of the cellular microenvironment, and provides a novel mechanistic insight into the pathobiology of not only EndMT in IPF, but also other traumatic fibrotic disease disorders.
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spelling pubmed-90963612022-05-13 Heterogeneous microenvironment analysis to explore the potential regulatory role of endothelial-mesenchymal transition in idiopathic pulmonary fibrosis Xu, Yinning Hu, Xiaoping Zhang, Yiwen Pan, Zongfu Sun, Zhiyong Huang, Zhongjie Zheng, Shuilian Pan, Hansheng Zou, Xiaozhou Huang, Ping Ann Transl Med Original Article BACKGROUND: Idiopathic pulmonary fibrosis (IPF) is a chronic and progressive interstitial lung disease mainly caused by excessive proliferation of fibroblasts and activation of myofibroblasts. The cellular microenvironment is mainly composed of different types of cellular components and extracellular matrix (ECM), whose changes directly affect cellular heterogeneity, resulting in immensely complex cellular interactions. However, microenvironment study is mainly focused on the pathological process of tumors, and the microenvironment changes during IPF development remain unclear. METHODS: The current study intends to employ IPF-related single-cell sequencing and gene expression profile data to analyze the scores of different cell clusters in the IPF microenvironment, and exploit the underlying interaction between cells to illustrate the fundamental mechanism causing IPF. RESULTS: Our analysis revealed that the amount of endothelial cells was obviously decreased, and the amount of fibroblasts and myofibroblasts was increased during the development of IPF, suggesting a possible endothelial-mesenchymal transition (EndMT) process. Furthermore, we found that the hub genes obtained through IPF-related gene expression profile analysis may play a regulative role in the number and function of endothelial cells and fibroblasts/myofibroblasts during IPF. CONCLUSIONS: Our research represents a valuable analysis of the cellular microenvironment, and provides a novel mechanistic insight into the pathobiology of not only EndMT in IPF, but also other traumatic fibrotic disease disorders. AME Publishing Company 2022-04 /pmc/articles/PMC9096361/ /pubmed/35571445 http://dx.doi.org/10.21037/atm-22-1438 Text en 2022 Annals of Translational Medicine. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Original Article
Xu, Yinning
Hu, Xiaoping
Zhang, Yiwen
Pan, Zongfu
Sun, Zhiyong
Huang, Zhongjie
Zheng, Shuilian
Pan, Hansheng
Zou, Xiaozhou
Huang, Ping
Heterogeneous microenvironment analysis to explore the potential regulatory role of endothelial-mesenchymal transition in idiopathic pulmonary fibrosis
title Heterogeneous microenvironment analysis to explore the potential regulatory role of endothelial-mesenchymal transition in idiopathic pulmonary fibrosis
title_full Heterogeneous microenvironment analysis to explore the potential regulatory role of endothelial-mesenchymal transition in idiopathic pulmonary fibrosis
title_fullStr Heterogeneous microenvironment analysis to explore the potential regulatory role of endothelial-mesenchymal transition in idiopathic pulmonary fibrosis
title_full_unstemmed Heterogeneous microenvironment analysis to explore the potential regulatory role of endothelial-mesenchymal transition in idiopathic pulmonary fibrosis
title_short Heterogeneous microenvironment analysis to explore the potential regulatory role of endothelial-mesenchymal transition in idiopathic pulmonary fibrosis
title_sort heterogeneous microenvironment analysis to explore the potential regulatory role of endothelial-mesenchymal transition in idiopathic pulmonary fibrosis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9096361/
https://www.ncbi.nlm.nih.gov/pubmed/35571445
http://dx.doi.org/10.21037/atm-22-1438
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