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Spectroscopic photoacoustic microscopic imaging during single spatial scan using broadband excitation light pulses with wavelength-dependent time delay

In most multispectral optical-resolution photoacoustic microscopy (OR-PAM), spatial scanning is repeated for each excitation wavelength, which decreases throughput and causes motion artifacts during spectral processing. This study proposes a new spectroscopic OR-PAM technique to acquire information...

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Detalles Bibliográficos
Autores principales: Hirasawa, Takeshi, Tachi, Kazuyoshi, Miyashita, Manami, Okawa, Shinpei, Kushibiki, Toshihiro, Ishihara, Miya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9096666/
https://www.ncbi.nlm.nih.gov/pubmed/35574189
http://dx.doi.org/10.1016/j.pacs.2022.100364
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author Hirasawa, Takeshi
Tachi, Kazuyoshi
Miyashita, Manami
Okawa, Shinpei
Kushibiki, Toshihiro
Ishihara, Miya
author_facet Hirasawa, Takeshi
Tachi, Kazuyoshi
Miyashita, Manami
Okawa, Shinpei
Kushibiki, Toshihiro
Ishihara, Miya
author_sort Hirasawa, Takeshi
collection PubMed
description In most multispectral optical-resolution photoacoustic microscopy (OR-PAM), spatial scanning is repeated for each excitation wavelength, which decreases throughput and causes motion artifacts during spectral processing. This study proposes a new spectroscopic OR-PAM technique to acquire information on the photoacoustic signal intensity and excitation wavelength from single spatial scans. The technique involves irradiating an imaging target with two broadband optical pulses with and without wavelength-dependent time delays. The excitation wavelength of the sample is then calculated by measuring the time delay between the photoacoustic signals generated by the two optical pulses. This technique is validated by measuring the excitation wavelengths of dyes in tubes. Furthermore, we demonstrate the three-dimensional spectroscopic OR-PAM of cells stained with suitable dyes. Although the tradeoff between excitation efficiency and excitation bandwidth must be adjusted based on the application, combining the proposed technique with fast spatial scanning methods can significantly contribute to recent OR-PAM applications, such as monitoring quick biological events and microscale tracking of moving materials.
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spelling pubmed-90966662022-05-13 Spectroscopic photoacoustic microscopic imaging during single spatial scan using broadband excitation light pulses with wavelength-dependent time delay Hirasawa, Takeshi Tachi, Kazuyoshi Miyashita, Manami Okawa, Shinpei Kushibiki, Toshihiro Ishihara, Miya Photoacoustics Research Article In most multispectral optical-resolution photoacoustic microscopy (OR-PAM), spatial scanning is repeated for each excitation wavelength, which decreases throughput and causes motion artifacts during spectral processing. This study proposes a new spectroscopic OR-PAM technique to acquire information on the photoacoustic signal intensity and excitation wavelength from single spatial scans. The technique involves irradiating an imaging target with two broadband optical pulses with and without wavelength-dependent time delays. The excitation wavelength of the sample is then calculated by measuring the time delay between the photoacoustic signals generated by the two optical pulses. This technique is validated by measuring the excitation wavelengths of dyes in tubes. Furthermore, we demonstrate the three-dimensional spectroscopic OR-PAM of cells stained with suitable dyes. Although the tradeoff between excitation efficiency and excitation bandwidth must be adjusted based on the application, combining the proposed technique with fast spatial scanning methods can significantly contribute to recent OR-PAM applications, such as monitoring quick biological events and microscale tracking of moving materials. Elsevier 2022-05-07 /pmc/articles/PMC9096666/ /pubmed/35574189 http://dx.doi.org/10.1016/j.pacs.2022.100364 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Hirasawa, Takeshi
Tachi, Kazuyoshi
Miyashita, Manami
Okawa, Shinpei
Kushibiki, Toshihiro
Ishihara, Miya
Spectroscopic photoacoustic microscopic imaging during single spatial scan using broadband excitation light pulses with wavelength-dependent time delay
title Spectroscopic photoacoustic microscopic imaging during single spatial scan using broadband excitation light pulses with wavelength-dependent time delay
title_full Spectroscopic photoacoustic microscopic imaging during single spatial scan using broadband excitation light pulses with wavelength-dependent time delay
title_fullStr Spectroscopic photoacoustic microscopic imaging during single spatial scan using broadband excitation light pulses with wavelength-dependent time delay
title_full_unstemmed Spectroscopic photoacoustic microscopic imaging during single spatial scan using broadband excitation light pulses with wavelength-dependent time delay
title_short Spectroscopic photoacoustic microscopic imaging during single spatial scan using broadband excitation light pulses with wavelength-dependent time delay
title_sort spectroscopic photoacoustic microscopic imaging during single spatial scan using broadband excitation light pulses with wavelength-dependent time delay
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9096666/
https://www.ncbi.nlm.nih.gov/pubmed/35574189
http://dx.doi.org/10.1016/j.pacs.2022.100364
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