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XNAs: A Troubleshooter for Nucleic Acid Sensing
[Image: see text] The strategies for nucleic acid sensing based on nucleic acid hybridization between the target sequence and the capture probe sequence are considered to be largely successful as far as detection of a specific target of known sequence is concerned. However, when compared with other...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9096816/ https://www.ncbi.nlm.nih.gov/pubmed/35571783 http://dx.doi.org/10.1021/acsomega.2c00581 |
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author | Mana, Tanushree Bhattacharya, Budhaditya Lahiri, Hiya Mukhopadhyay, Rupa |
author_facet | Mana, Tanushree Bhattacharya, Budhaditya Lahiri, Hiya Mukhopadhyay, Rupa |
author_sort | Mana, Tanushree |
collection | PubMed |
description | [Image: see text] The strategies for nucleic acid sensing based on nucleic acid hybridization between the target sequence and the capture probe sequence are considered to be largely successful as far as detection of a specific target of known sequence is concerned. However, when compared with other complementary methods, like direct sequencing, a number of results are still found to be either “false positives” or “false negatives”. This suggests that modifications in these strategies are necessary to make them more accurate. In this minireview, we propose that one way toward improvement could be replacement of the DNA capture probes with the xeno nucleic acid or XNA capture probes. This is because the XNAs, especially the locked nucleic acid, the peptide nucleic acid, and the morpholino, have shown better single nucleobase mismatch discrimination capacity than the DNA capture probes, indicating their capacity for more precise detection of nucleic acid sequences, which is beneficial for detection of gene stretches having point mutations. Keeping the current trend in mind, this minireview will include the recent developments in nanoscale, fluorescent label-free applications, and present the cases where the XNA probes show clear advantages over the DNA probes. |
format | Online Article Text |
id | pubmed-9096816 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-90968162022-05-13 XNAs: A Troubleshooter for Nucleic Acid Sensing Mana, Tanushree Bhattacharya, Budhaditya Lahiri, Hiya Mukhopadhyay, Rupa ACS Omega [Image: see text] The strategies for nucleic acid sensing based on nucleic acid hybridization between the target sequence and the capture probe sequence are considered to be largely successful as far as detection of a specific target of known sequence is concerned. However, when compared with other complementary methods, like direct sequencing, a number of results are still found to be either “false positives” or “false negatives”. This suggests that modifications in these strategies are necessary to make them more accurate. In this minireview, we propose that one way toward improvement could be replacement of the DNA capture probes with the xeno nucleic acid or XNA capture probes. This is because the XNAs, especially the locked nucleic acid, the peptide nucleic acid, and the morpholino, have shown better single nucleobase mismatch discrimination capacity than the DNA capture probes, indicating their capacity for more precise detection of nucleic acid sequences, which is beneficial for detection of gene stretches having point mutations. Keeping the current trend in mind, this minireview will include the recent developments in nanoscale, fluorescent label-free applications, and present the cases where the XNA probes show clear advantages over the DNA probes. American Chemical Society 2022-04-28 /pmc/articles/PMC9096816/ /pubmed/35571783 http://dx.doi.org/10.1021/acsomega.2c00581 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Mana, Tanushree Bhattacharya, Budhaditya Lahiri, Hiya Mukhopadhyay, Rupa XNAs: A Troubleshooter for Nucleic Acid Sensing |
title | XNAs: A Troubleshooter for Nucleic Acid Sensing |
title_full | XNAs: A Troubleshooter for Nucleic Acid Sensing |
title_fullStr | XNAs: A Troubleshooter for Nucleic Acid Sensing |
title_full_unstemmed | XNAs: A Troubleshooter for Nucleic Acid Sensing |
title_short | XNAs: A Troubleshooter for Nucleic Acid Sensing |
title_sort | xnas: a troubleshooter for nucleic acid sensing |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9096816/ https://www.ncbi.nlm.nih.gov/pubmed/35571783 http://dx.doi.org/10.1021/acsomega.2c00581 |
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