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Chrysanthemum × grandiflora leaf and root transcript profiling in response to salinity stress

As high soil salinity threatens the growth and development of plants, understanding the mechanism of plants’ salt tolerance is critical. The Chrysanthemum × grandiflora is a newly developed species with a strong salt resistance that possesses multiple genes controlling its quantitative salt resistan...

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Autores principales: Liu, He, Liu, Yu, Xu, Ning, Sun, Ying, Li, Qiang, Yue, Liran, Zhou, Yunwei, He, Miao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9097105/
https://www.ncbi.nlm.nih.gov/pubmed/35549680
http://dx.doi.org/10.1186/s12870-022-03612-x
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author Liu, He
Liu, Yu
Xu, Ning
Sun, Ying
Li, Qiang
Yue, Liran
Zhou, Yunwei
He, Miao
author_facet Liu, He
Liu, Yu
Xu, Ning
Sun, Ying
Li, Qiang
Yue, Liran
Zhou, Yunwei
He, Miao
author_sort Liu, He
collection PubMed
description As high soil salinity threatens the growth and development of plants, understanding the mechanism of plants’ salt tolerance is critical. The Chrysanthemum × grandiflora is a newly developed species with a strong salt resistance that possesses multiple genes controlling its quantitative salt resistance. Because of this multigene control, we chose to investigate the plant stress genes overall responses at the transcriptome level. C. grandiflora were treated with a 200 mM NaCl solution for 12 h to study its effect on the roots and leaves via Illumina RNA sequencing. PAL, CYP73A, and 4CL in the phenylpropanoid biosynthesis pathway were upregulated in roots and leaves. In the salicylic acid signal transduction pathway, TGA7 was upregulated in the roots and leaves, while in the jasmonic acid signal transduction pathway, TIFY9 was upregulated in the roots and leaves. In the ion transporter gene, we identified HKT1 that showed identical expression patterns in the roots and leaves. The impact of NaCl imposition for 12 h was largely due to osmotic effect of salinity on C. grandiflora, and most likely the transcript abundance changes in this study were due to the osmotic effect. In order to verify the accuracy of the Illumina sequencing data, we selected 16 DEGs for transcription polymerase chain reaction (qRT-PCR) analysis. qRT-PCR and transcriptome sequencing analysis revealed that the transcriptome sequencing results were reliable. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-022-03612-x.
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spelling pubmed-90971052022-05-13 Chrysanthemum × grandiflora leaf and root transcript profiling in response to salinity stress Liu, He Liu, Yu Xu, Ning Sun, Ying Li, Qiang Yue, Liran Zhou, Yunwei He, Miao BMC Plant Biol Research As high soil salinity threatens the growth and development of plants, understanding the mechanism of plants’ salt tolerance is critical. The Chrysanthemum × grandiflora is a newly developed species with a strong salt resistance that possesses multiple genes controlling its quantitative salt resistance. Because of this multigene control, we chose to investigate the plant stress genes overall responses at the transcriptome level. C. grandiflora were treated with a 200 mM NaCl solution for 12 h to study its effect on the roots and leaves via Illumina RNA sequencing. PAL, CYP73A, and 4CL in the phenylpropanoid biosynthesis pathway were upregulated in roots and leaves. In the salicylic acid signal transduction pathway, TGA7 was upregulated in the roots and leaves, while in the jasmonic acid signal transduction pathway, TIFY9 was upregulated in the roots and leaves. In the ion transporter gene, we identified HKT1 that showed identical expression patterns in the roots and leaves. The impact of NaCl imposition for 12 h was largely due to osmotic effect of salinity on C. grandiflora, and most likely the transcript abundance changes in this study were due to the osmotic effect. In order to verify the accuracy of the Illumina sequencing data, we selected 16 DEGs for transcription polymerase chain reaction (qRT-PCR) analysis. qRT-PCR and transcriptome sequencing analysis revealed that the transcriptome sequencing results were reliable. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-022-03612-x. BioMed Central 2022-05-12 /pmc/articles/PMC9097105/ /pubmed/35549680 http://dx.doi.org/10.1186/s12870-022-03612-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Liu, He
Liu, Yu
Xu, Ning
Sun, Ying
Li, Qiang
Yue, Liran
Zhou, Yunwei
He, Miao
Chrysanthemum × grandiflora leaf and root transcript profiling in response to salinity stress
title Chrysanthemum × grandiflora leaf and root transcript profiling in response to salinity stress
title_full Chrysanthemum × grandiflora leaf and root transcript profiling in response to salinity stress
title_fullStr Chrysanthemum × grandiflora leaf and root transcript profiling in response to salinity stress
title_full_unstemmed Chrysanthemum × grandiflora leaf and root transcript profiling in response to salinity stress
title_short Chrysanthemum × grandiflora leaf and root transcript profiling in response to salinity stress
title_sort chrysanthemum × grandiflora leaf and root transcript profiling in response to salinity stress
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9097105/
https://www.ncbi.nlm.nih.gov/pubmed/35549680
http://dx.doi.org/10.1186/s12870-022-03612-x
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