Cargando…
Chrysanthemum × grandiflora leaf and root transcript profiling in response to salinity stress
As high soil salinity threatens the growth and development of plants, understanding the mechanism of plants’ salt tolerance is critical. The Chrysanthemum × grandiflora is a newly developed species with a strong salt resistance that possesses multiple genes controlling its quantitative salt resistan...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9097105/ https://www.ncbi.nlm.nih.gov/pubmed/35549680 http://dx.doi.org/10.1186/s12870-022-03612-x |
_version_ | 1784706110496702464 |
---|---|
author | Liu, He Liu, Yu Xu, Ning Sun, Ying Li, Qiang Yue, Liran Zhou, Yunwei He, Miao |
author_facet | Liu, He Liu, Yu Xu, Ning Sun, Ying Li, Qiang Yue, Liran Zhou, Yunwei He, Miao |
author_sort | Liu, He |
collection | PubMed |
description | As high soil salinity threatens the growth and development of plants, understanding the mechanism of plants’ salt tolerance is critical. The Chrysanthemum × grandiflora is a newly developed species with a strong salt resistance that possesses multiple genes controlling its quantitative salt resistance. Because of this multigene control, we chose to investigate the plant stress genes overall responses at the transcriptome level. C. grandiflora were treated with a 200 mM NaCl solution for 12 h to study its effect on the roots and leaves via Illumina RNA sequencing. PAL, CYP73A, and 4CL in the phenylpropanoid biosynthesis pathway were upregulated in roots and leaves. In the salicylic acid signal transduction pathway, TGA7 was upregulated in the roots and leaves, while in the jasmonic acid signal transduction pathway, TIFY9 was upregulated in the roots and leaves. In the ion transporter gene, we identified HKT1 that showed identical expression patterns in the roots and leaves. The impact of NaCl imposition for 12 h was largely due to osmotic effect of salinity on C. grandiflora, and most likely the transcript abundance changes in this study were due to the osmotic effect. In order to verify the accuracy of the Illumina sequencing data, we selected 16 DEGs for transcription polymerase chain reaction (qRT-PCR) analysis. qRT-PCR and transcriptome sequencing analysis revealed that the transcriptome sequencing results were reliable. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-022-03612-x. |
format | Online Article Text |
id | pubmed-9097105 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-90971052022-05-13 Chrysanthemum × grandiflora leaf and root transcript profiling in response to salinity stress Liu, He Liu, Yu Xu, Ning Sun, Ying Li, Qiang Yue, Liran Zhou, Yunwei He, Miao BMC Plant Biol Research As high soil salinity threatens the growth and development of plants, understanding the mechanism of plants’ salt tolerance is critical. The Chrysanthemum × grandiflora is a newly developed species with a strong salt resistance that possesses multiple genes controlling its quantitative salt resistance. Because of this multigene control, we chose to investigate the plant stress genes overall responses at the transcriptome level. C. grandiflora were treated with a 200 mM NaCl solution for 12 h to study its effect on the roots and leaves via Illumina RNA sequencing. PAL, CYP73A, and 4CL in the phenylpropanoid biosynthesis pathway were upregulated in roots and leaves. In the salicylic acid signal transduction pathway, TGA7 was upregulated in the roots and leaves, while in the jasmonic acid signal transduction pathway, TIFY9 was upregulated in the roots and leaves. In the ion transporter gene, we identified HKT1 that showed identical expression patterns in the roots and leaves. The impact of NaCl imposition for 12 h was largely due to osmotic effect of salinity on C. grandiflora, and most likely the transcript abundance changes in this study were due to the osmotic effect. In order to verify the accuracy of the Illumina sequencing data, we selected 16 DEGs for transcription polymerase chain reaction (qRT-PCR) analysis. qRT-PCR and transcriptome sequencing analysis revealed that the transcriptome sequencing results were reliable. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-022-03612-x. BioMed Central 2022-05-12 /pmc/articles/PMC9097105/ /pubmed/35549680 http://dx.doi.org/10.1186/s12870-022-03612-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Liu, He Liu, Yu Xu, Ning Sun, Ying Li, Qiang Yue, Liran Zhou, Yunwei He, Miao Chrysanthemum × grandiflora leaf and root transcript profiling in response to salinity stress |
title | Chrysanthemum × grandiflora leaf and root transcript profiling in response to salinity stress |
title_full | Chrysanthemum × grandiflora leaf and root transcript profiling in response to salinity stress |
title_fullStr | Chrysanthemum × grandiflora leaf and root transcript profiling in response to salinity stress |
title_full_unstemmed | Chrysanthemum × grandiflora leaf and root transcript profiling in response to salinity stress |
title_short | Chrysanthemum × grandiflora leaf and root transcript profiling in response to salinity stress |
title_sort | chrysanthemum × grandiflora leaf and root transcript profiling in response to salinity stress |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9097105/ https://www.ncbi.nlm.nih.gov/pubmed/35549680 http://dx.doi.org/10.1186/s12870-022-03612-x |
work_keys_str_mv | AT liuhe chrysanthemumgrandifloraleafandroottranscriptprofilinginresponsetosalinitystress AT liuyu chrysanthemumgrandifloraleafandroottranscriptprofilinginresponsetosalinitystress AT xuning chrysanthemumgrandifloraleafandroottranscriptprofilinginresponsetosalinitystress AT sunying chrysanthemumgrandifloraleafandroottranscriptprofilinginresponsetosalinitystress AT liqiang chrysanthemumgrandifloraleafandroottranscriptprofilinginresponsetosalinitystress AT yueliran chrysanthemumgrandifloraleafandroottranscriptprofilinginresponsetosalinitystress AT zhouyunwei chrysanthemumgrandifloraleafandroottranscriptprofilinginresponsetosalinitystress AT hemiao chrysanthemumgrandifloraleafandroottranscriptprofilinginresponsetosalinitystress |