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Immunohistochemical identification and assessment of the location of leptin, visfatin and chemerin in the liver of men with different body mass index
BACKGROUND: Adipokines such as leptin, visfatin and chemerin play a pivotal role not only in the pathogenesis of excessive weight gain but also impact on hepatic metabolism. However, alterations in the production of these peptides in the liver of overweight individuals have not been fully elucidated...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9097377/ https://www.ncbi.nlm.nih.gov/pubmed/35549673 http://dx.doi.org/10.1186/s12876-022-02299-6 |
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author | Kasacka, I. Piotrowska, Ż. Domian, N. Lewandowska, A. Acewicz, M. |
author_facet | Kasacka, I. Piotrowska, Ż. Domian, N. Lewandowska, A. Acewicz, M. |
author_sort | Kasacka, I. |
collection | PubMed |
description | BACKGROUND: Adipokines such as leptin, visfatin and chemerin play a pivotal role not only in the pathogenesis of excessive weight gain but also impact on hepatic metabolism. However, alterations in the production of these peptides in the liver of overweight individuals have not been fully elucidated yet. The aim of the study was to evaluate changes in leptin, visfatin and chemerin biosynthesis in the liver of men with different BMI. METHODS: Fourteen adult men without symptoms from the digestive system were recruited. Research material consisted of liver samples. Study participants were divided into two groups: lean (BMI ≤ 25 kg/m(2)) and overweight subjects (BMI > 25 kg/m(2)). Paraffin liver sections were processed by immunohistochemistry for detection of leptin, visfatin and chemerin. Hepatic expression of leptin, visfatin and chemerin genes was determined by qRT-PCR method. RESULTS: Increased immunoreactivity for leptin and chemerin, and decreased immunoreaction for visfatin were observed in the liver of overweight men in comparison to lean subjects. Overweight subjects with hepatic steatosis displayed increased immunoreactivity for leptin and weaker immunoreaction against visfatin and chemerin in the liver, compared to individuals with normal organ structure. Expression of leptin and chemerin was enhanced in the liver of overweight individuals, with the highest expression observed in subjects with hepatic steatosis. Conversely, expression of visfatin in the male liver was decreased in overweight subjects and those with and liver steatosis. CONCLUSIONS: The present study proves that the expression of leptin, visfatin and chemerin in the male liver is altered in overweight individuals. Our report also indicates the potential importance of these peptides in hepatic steatosis associated with overweight. |
format | Online Article Text |
id | pubmed-9097377 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-90973772022-05-13 Immunohistochemical identification and assessment of the location of leptin, visfatin and chemerin in the liver of men with different body mass index Kasacka, I. Piotrowska, Ż. Domian, N. Lewandowska, A. Acewicz, M. BMC Gastroenterol Research BACKGROUND: Adipokines such as leptin, visfatin and chemerin play a pivotal role not only in the pathogenesis of excessive weight gain but also impact on hepatic metabolism. However, alterations in the production of these peptides in the liver of overweight individuals have not been fully elucidated yet. The aim of the study was to evaluate changes in leptin, visfatin and chemerin biosynthesis in the liver of men with different BMI. METHODS: Fourteen adult men without symptoms from the digestive system were recruited. Research material consisted of liver samples. Study participants were divided into two groups: lean (BMI ≤ 25 kg/m(2)) and overweight subjects (BMI > 25 kg/m(2)). Paraffin liver sections were processed by immunohistochemistry for detection of leptin, visfatin and chemerin. Hepatic expression of leptin, visfatin and chemerin genes was determined by qRT-PCR method. RESULTS: Increased immunoreactivity for leptin and chemerin, and decreased immunoreaction for visfatin were observed in the liver of overweight men in comparison to lean subjects. Overweight subjects with hepatic steatosis displayed increased immunoreactivity for leptin and weaker immunoreaction against visfatin and chemerin in the liver, compared to individuals with normal organ structure. Expression of leptin and chemerin was enhanced in the liver of overweight individuals, with the highest expression observed in subjects with hepatic steatosis. Conversely, expression of visfatin in the male liver was decreased in overweight subjects and those with and liver steatosis. CONCLUSIONS: The present study proves that the expression of leptin, visfatin and chemerin in the male liver is altered in overweight individuals. Our report also indicates the potential importance of these peptides in hepatic steatosis associated with overweight. BioMed Central 2022-05-12 /pmc/articles/PMC9097377/ /pubmed/35549673 http://dx.doi.org/10.1186/s12876-022-02299-6 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Kasacka, I. Piotrowska, Ż. Domian, N. Lewandowska, A. Acewicz, M. Immunohistochemical identification and assessment of the location of leptin, visfatin and chemerin in the liver of men with different body mass index |
title | Immunohistochemical identification and assessment of the location of leptin, visfatin and chemerin in the liver of men with different body mass index |
title_full | Immunohistochemical identification and assessment of the location of leptin, visfatin and chemerin in the liver of men with different body mass index |
title_fullStr | Immunohistochemical identification and assessment of the location of leptin, visfatin and chemerin in the liver of men with different body mass index |
title_full_unstemmed | Immunohistochemical identification and assessment of the location of leptin, visfatin and chemerin in the liver of men with different body mass index |
title_short | Immunohistochemical identification and assessment of the location of leptin, visfatin and chemerin in the liver of men with different body mass index |
title_sort | immunohistochemical identification and assessment of the location of leptin, visfatin and chemerin in the liver of men with different body mass index |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9097377/ https://www.ncbi.nlm.nih.gov/pubmed/35549673 http://dx.doi.org/10.1186/s12876-022-02299-6 |
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