Introduction of loxP sites by electroporation in the mouse genome; a simple approach for conditional allele generation in complex targeting loci

BACKGROUND: The discovery of the CRISPR-Cas9 system and its applicability in mammalian embryos has revolutionized the way we generate genetically engineered animal models. To date, models harbouring conditional alleles (i.e. two loxP sites flanking an exon or a critical DNA sequence of interest) are...

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Detalles Bibliográficos
Autores principales: Bernas, Guillaume, Ouellet, Mariette, Barrios, Andréa, Jamann, Hélène, Larochelle, Catherine, Lévy, Émile, Schmouth, Jean-François
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9097428/
https://www.ncbi.nlm.nih.gov/pubmed/35549895
http://dx.doi.org/10.1186/s12896-022-00744-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9097428/
https://www.ncbi.nlm.nih.gov/pubmed/35549895
http://dx.doi.org/10.1186/s12896-022-00744-8

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