Analysis of the interplay between hepatitis B virus-positive hepatocytes and Kupffer cells ex vivo using mice as a model

Kupffer cells play critical roles in both hepatitis B virus (HBV) persistence and clearance. Here, we provide a protocol for studying the interplay between Kupffer cells and HBV-positive hepatocytes ex vivo using mice as a model. This protocol includes hydrodynamic injection of HBV DNA into mouse he...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Yumei, Lee, Jiyoung, Helou, Doumet Georges, Akbari, Omid, Ou, Jing-hsiung James
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9097500/
https://www.ncbi.nlm.nih.gov/pubmed/35573478
http://dx.doi.org/10.1016/j.xpro.2022.101364
_version_ 1784706189855031296
author Li, Yumei
Lee, Jiyoung
Helou, Doumet Georges
Akbari, Omid
Ou, Jing-hsiung James
author_facet Li, Yumei
Lee, Jiyoung
Helou, Doumet Georges
Akbari, Omid
Ou, Jing-hsiung James
author_sort Li, Yumei
collection PubMed
description Kupffer cells play critical roles in both hepatitis B virus (HBV) persistence and clearance. Here, we provide a protocol for studying the interplay between Kupffer cells and HBV-positive hepatocytes ex vivo using mice as a model. This protocol includes hydrodynamic injection of HBV DNA into mouse hepatocytes, liver perfusion for isolating hepatocytes and Kupffer cells, and Seahorse metabolic analysis of Kupffer cells. This protocol allows the detailed analysis of how HBV-positive hepatocytes and Kupffer cells impact each other ex vivo. For complete details on the use and execution of this protocol, please refer to Li et al. (2022).
format Online
Article
Text
id pubmed-9097500
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-90975002022-05-13 Analysis of the interplay between hepatitis B virus-positive hepatocytes and Kupffer cells ex vivo using mice as a model Li, Yumei Lee, Jiyoung Helou, Doumet Georges Akbari, Omid Ou, Jing-hsiung James STAR Protoc Protocol Kupffer cells play critical roles in both hepatitis B virus (HBV) persistence and clearance. Here, we provide a protocol for studying the interplay between Kupffer cells and HBV-positive hepatocytes ex vivo using mice as a model. This protocol includes hydrodynamic injection of HBV DNA into mouse hepatocytes, liver perfusion for isolating hepatocytes and Kupffer cells, and Seahorse metabolic analysis of Kupffer cells. This protocol allows the detailed analysis of how HBV-positive hepatocytes and Kupffer cells impact each other ex vivo. For complete details on the use and execution of this protocol, please refer to Li et al. (2022). Elsevier 2022-05-06 /pmc/articles/PMC9097500/ /pubmed/35573478 http://dx.doi.org/10.1016/j.xpro.2022.101364 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Li, Yumei
Lee, Jiyoung
Helou, Doumet Georges
Akbari, Omid
Ou, Jing-hsiung James
Analysis of the interplay between hepatitis B virus-positive hepatocytes and Kupffer cells ex vivo using mice as a model
title Analysis of the interplay between hepatitis B virus-positive hepatocytes and Kupffer cells ex vivo using mice as a model
title_full Analysis of the interplay between hepatitis B virus-positive hepatocytes and Kupffer cells ex vivo using mice as a model
title_fullStr Analysis of the interplay between hepatitis B virus-positive hepatocytes and Kupffer cells ex vivo using mice as a model
title_full_unstemmed Analysis of the interplay between hepatitis B virus-positive hepatocytes and Kupffer cells ex vivo using mice as a model
title_short Analysis of the interplay between hepatitis B virus-positive hepatocytes and Kupffer cells ex vivo using mice as a model
title_sort analysis of the interplay between hepatitis b virus-positive hepatocytes and kupffer cells ex vivo using mice as a model
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9097500/
https://www.ncbi.nlm.nih.gov/pubmed/35573478
http://dx.doi.org/10.1016/j.xpro.2022.101364
work_keys_str_mv AT liyumei analysisoftheinterplaybetweenhepatitisbviruspositivehepatocytesandkupffercellsexvivousingmiceasamodel
AT leejiyoung analysisoftheinterplaybetweenhepatitisbviruspositivehepatocytesandkupffercellsexvivousingmiceasamodel
AT heloudoumetgeorges analysisoftheinterplaybetweenhepatitisbviruspositivehepatocytesandkupffercellsexvivousingmiceasamodel
AT akbariomid analysisoftheinterplaybetweenhepatitisbviruspositivehepatocytesandkupffercellsexvivousingmiceasamodel
AT oujinghsiungjames analysisoftheinterplaybetweenhepatitisbviruspositivehepatocytesandkupffercellsexvivousingmiceasamodel

Ejemplares similares