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Propionic Acid, Induced in Gut by an Inulin Diet, Suppresses Inflammation and Ameliorates Liver Ischemia and Reperfusion Injury in Mice

Liver ischemia and reperfusion injury (IRI) is one of the obstacles in liver surgery such as liver resection and transplantation. In this study, we investigated the preventive effect on mouse liver IRI by feeding mice with inulin, which is a heterogeneous blend of indigestible fructose polymer. Mice...

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Autores principales: Kawasoe, Junya, Uchida, Yoichiro, Kawamoto, Hiroshi, Miyauchi, Tomoyuki, Watanabe, Takeshi, Saga, Kenichi, Tanaka, Kosuke, Ueda, Shugo, Terajima, Hiroaki, Taura, Kojiro, Hatano, Etsuro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9097600/
https://www.ncbi.nlm.nih.gov/pubmed/35572528
http://dx.doi.org/10.3389/fimmu.2022.862503
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author Kawasoe, Junya
Uchida, Yoichiro
Kawamoto, Hiroshi
Miyauchi, Tomoyuki
Watanabe, Takeshi
Saga, Kenichi
Tanaka, Kosuke
Ueda, Shugo
Terajima, Hiroaki
Taura, Kojiro
Hatano, Etsuro
author_facet Kawasoe, Junya
Uchida, Yoichiro
Kawamoto, Hiroshi
Miyauchi, Tomoyuki
Watanabe, Takeshi
Saga, Kenichi
Tanaka, Kosuke
Ueda, Shugo
Terajima, Hiroaki
Taura, Kojiro
Hatano, Etsuro
author_sort Kawasoe, Junya
collection PubMed
description Liver ischemia and reperfusion injury (IRI) is one of the obstacles in liver surgery such as liver resection and transplantation. In this study, we investigated the preventive effect on mouse liver IRI by feeding mice with inulin, which is a heterogeneous blend of indigestible fructose polymer. Mice were fed either a control ordinary diet (CD) or an inulin diet (ID) containing 5% inulin in the CD, for 14 days before the ischemia and reperfusion (IR) maneuver. IR induced-liver damages were significantly ameliorated in the ID group, compared with those in the CD group. Feeding mice with an ID, but not a CD, elevated levels of Bacteroidetes among gut microbiota, and especially increased Bacteroides acidifaciens in mouse feces, which resulted in significant elevation of short-chain fatty acids (SCFAs) in the portal vein of mice. Among SCFAs, propionic acid (PA) was most significantly increased. The microbial gene functions related to PA biosynthesis were much higher in the fecal microbiome of the ID group compared to the CD. However, the action of PA on liver IRI has not been yet clarified. Direct intraperitoneal administration of PA alone prior to the ischemia strongly suppressed liver cell damages as well as inflammatory responses caused by liver IR. Furthermore, PA suppressed the secretion of inflammatory cytokines from peritoneal macrophages stimulated in vitro through TLR-4 with high-mobility group box 1 protein (HMGB-1), known to be released from apoptotic liver cells during the IR insult. The present study shows that PA may play a key role in the inulin-induced amelioration of mouse liver IRI.
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spelling pubmed-90976002022-05-13 Propionic Acid, Induced in Gut by an Inulin Diet, Suppresses Inflammation and Ameliorates Liver Ischemia and Reperfusion Injury in Mice Kawasoe, Junya Uchida, Yoichiro Kawamoto, Hiroshi Miyauchi, Tomoyuki Watanabe, Takeshi Saga, Kenichi Tanaka, Kosuke Ueda, Shugo Terajima, Hiroaki Taura, Kojiro Hatano, Etsuro Front Immunol Immunology Liver ischemia and reperfusion injury (IRI) is one of the obstacles in liver surgery such as liver resection and transplantation. In this study, we investigated the preventive effect on mouse liver IRI by feeding mice with inulin, which is a heterogeneous blend of indigestible fructose polymer. Mice were fed either a control ordinary diet (CD) or an inulin diet (ID) containing 5% inulin in the CD, for 14 days before the ischemia and reperfusion (IR) maneuver. IR induced-liver damages were significantly ameliorated in the ID group, compared with those in the CD group. Feeding mice with an ID, but not a CD, elevated levels of Bacteroidetes among gut microbiota, and especially increased Bacteroides acidifaciens in mouse feces, which resulted in significant elevation of short-chain fatty acids (SCFAs) in the portal vein of mice. Among SCFAs, propionic acid (PA) was most significantly increased. The microbial gene functions related to PA biosynthesis were much higher in the fecal microbiome of the ID group compared to the CD. However, the action of PA on liver IRI has not been yet clarified. Direct intraperitoneal administration of PA alone prior to the ischemia strongly suppressed liver cell damages as well as inflammatory responses caused by liver IR. Furthermore, PA suppressed the secretion of inflammatory cytokines from peritoneal macrophages stimulated in vitro through TLR-4 with high-mobility group box 1 protein (HMGB-1), known to be released from apoptotic liver cells during the IR insult. The present study shows that PA may play a key role in the inulin-induced amelioration of mouse liver IRI. Frontiers Media S.A. 2022-04-22 /pmc/articles/PMC9097600/ /pubmed/35572528 http://dx.doi.org/10.3389/fimmu.2022.862503 Text en Copyright © 2022 Kawasoe, Uchida, Kawamoto, Miyauchi, Watanabe, Saga, Tanaka, Ueda, Terajima, Taura and Hatano https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Kawasoe, Junya
Uchida, Yoichiro
Kawamoto, Hiroshi
Miyauchi, Tomoyuki
Watanabe, Takeshi
Saga, Kenichi
Tanaka, Kosuke
Ueda, Shugo
Terajima, Hiroaki
Taura, Kojiro
Hatano, Etsuro
Propionic Acid, Induced in Gut by an Inulin Diet, Suppresses Inflammation and Ameliorates Liver Ischemia and Reperfusion Injury in Mice
title Propionic Acid, Induced in Gut by an Inulin Diet, Suppresses Inflammation and Ameliorates Liver Ischemia and Reperfusion Injury in Mice
title_full Propionic Acid, Induced in Gut by an Inulin Diet, Suppresses Inflammation and Ameliorates Liver Ischemia and Reperfusion Injury in Mice
title_fullStr Propionic Acid, Induced in Gut by an Inulin Diet, Suppresses Inflammation and Ameliorates Liver Ischemia and Reperfusion Injury in Mice
title_full_unstemmed Propionic Acid, Induced in Gut by an Inulin Diet, Suppresses Inflammation and Ameliorates Liver Ischemia and Reperfusion Injury in Mice
title_short Propionic Acid, Induced in Gut by an Inulin Diet, Suppresses Inflammation and Ameliorates Liver Ischemia and Reperfusion Injury in Mice
title_sort propionic acid, induced in gut by an inulin diet, suppresses inflammation and ameliorates liver ischemia and reperfusion injury in mice
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9097600/
https://www.ncbi.nlm.nih.gov/pubmed/35572528
http://dx.doi.org/10.3389/fimmu.2022.862503
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