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Expression profiles of iron transport molecules along the duodenum

Duodenal biopsies are considered a suitable source of enterocytes for studies of dietary iron absorption. However, the expression level of molecules involved in iron absorption may vary along the length of duodenum. We aimed to determine whether the expression of molecules involved in the absorption...

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Autores principales: Balusikova, Kamila, Dostalikova‐Cimburova, Marketa, Tacheci, Ilja, Kovar, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9097835/
https://www.ncbi.nlm.nih.gov/pubmed/35445529
http://dx.doi.org/10.1111/jcmm.17313
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author Balusikova, Kamila
Dostalikova‐Cimburova, Marketa
Tacheci, Ilja
Kovar, Jan
author_facet Balusikova, Kamila
Dostalikova‐Cimburova, Marketa
Tacheci, Ilja
Kovar, Jan
author_sort Balusikova, Kamila
collection PubMed
description Duodenal biopsies are considered a suitable source of enterocytes for studies of dietary iron absorption. However, the expression level of molecules involved in iron absorption may vary along the length of duodenum. We aimed to determine whether the expression of molecules involved in the absorption of heme and non‐heme iron differs depending on the location in the duodenum. Analysis was performed with samples of duodenal biopsies from 10 individuals with normal iron metabolism. Samples were collected at the following locations: (a) immediately post‐bulbar, (b) 1–2 cm below the papilla of Vater and (c) in the distal duodenum. The gene expression was analyzed at the mRNA and protein level using real‐time PCR and Western blot analysis. At the mRNA level, significantly different expression of HCP1, DMT1, ferroportin and Zip8 was found at individual positions of duodenum. Position‐dependent expression of other molecules, especially of FLVCR1, HMOX1 and HMOX2 was also detected but with no statistical significances. At the protein level, we observed statistically significantly decreasing expression of transporters HCP1, FLVCR1, DMT1, ferroportin, Zip14 and Zip8 with advancing positions of duodenum. Our results are consistent with a gradient of diminishing iron absorption along the duodenum for both heme and non‐heme iron.
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spelling pubmed-90978352022-05-18 Expression profiles of iron transport molecules along the duodenum Balusikova, Kamila Dostalikova‐Cimburova, Marketa Tacheci, Ilja Kovar, Jan J Cell Mol Med Original Articles Duodenal biopsies are considered a suitable source of enterocytes for studies of dietary iron absorption. However, the expression level of molecules involved in iron absorption may vary along the length of duodenum. We aimed to determine whether the expression of molecules involved in the absorption of heme and non‐heme iron differs depending on the location in the duodenum. Analysis was performed with samples of duodenal biopsies from 10 individuals with normal iron metabolism. Samples were collected at the following locations: (a) immediately post‐bulbar, (b) 1–2 cm below the papilla of Vater and (c) in the distal duodenum. The gene expression was analyzed at the mRNA and protein level using real‐time PCR and Western blot analysis. At the mRNA level, significantly different expression of HCP1, DMT1, ferroportin and Zip8 was found at individual positions of duodenum. Position‐dependent expression of other molecules, especially of FLVCR1, HMOX1 and HMOX2 was also detected but with no statistical significances. At the protein level, we observed statistically significantly decreasing expression of transporters HCP1, FLVCR1, DMT1, ferroportin, Zip14 and Zip8 with advancing positions of duodenum. Our results are consistent with a gradient of diminishing iron absorption along the duodenum for both heme and non‐heme iron. John Wiley and Sons Inc. 2022-04-21 2022-05 /pmc/articles/PMC9097835/ /pubmed/35445529 http://dx.doi.org/10.1111/jcmm.17313 Text en © 2022 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Balusikova, Kamila
Dostalikova‐Cimburova, Marketa
Tacheci, Ilja
Kovar, Jan
Expression profiles of iron transport molecules along the duodenum
title Expression profiles of iron transport molecules along the duodenum
title_full Expression profiles of iron transport molecules along the duodenum
title_fullStr Expression profiles of iron transport molecules along the duodenum
title_full_unstemmed Expression profiles of iron transport molecules along the duodenum
title_short Expression profiles of iron transport molecules along the duodenum
title_sort expression profiles of iron transport molecules along the duodenum
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9097835/
https://www.ncbi.nlm.nih.gov/pubmed/35445529
http://dx.doi.org/10.1111/jcmm.17313
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