Construction of a Dual Protease Column, Subzero (-30 °C) Chromatography System and Multi-channel Precision Temperature Controller for Hydrogen-Deuterium Exchange Mass Spectrometry

This tutorial provides mechanical drawings, electrical schematics, parts lists, stereolithography (STL) files for producing three-dimensional (3D)-printed parts, initial graphics exchange specification (IGS) files for automated machining, and instructions necessary for construction of a dual proteas...

Descripción completa

Detalles Bibliográficos
Autor principal: Hudgens, Jeffrey W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: [Gaithersburg, MD] : U.S. Dept. of Commerce, National Institute of Standards and Technology 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9097885/
https://www.ncbi.nlm.nih.gov/pubmed/35573859
http://dx.doi.org/10.6028/jres.125.025
_version_ 1784706263759716352
author Hudgens, Jeffrey W.
author_facet Hudgens, Jeffrey W.
author_sort Hudgens, Jeffrey W.
collection PubMed
description This tutorial provides mechanical drawings, electrical schematics, parts lists, stereolithography (STL) files for producing three-dimensional (3D)-printed parts, initial graphics exchange specification (IGS) files for automated machining, and instructions necessary for construction of a dual protease column, subzero, liquid chromatography system for hydrogen-deuterium exchange mass spectrometry (HDX-MS). Electro-mechanical schematics for construction of two multi-zone temperature controllers that regulate to ±0.05 oC are also included in this tutorial.
format Online
Article
Text
id pubmed-9097885
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher [Gaithersburg, MD] : U.S. Dept. of Commerce, National Institute of Standards and Technology
record_format MEDLINE/PubMed
spelling pubmed-90978852022-05-13 Construction of a Dual Protease Column, Subzero (-30 °C) Chromatography System and Multi-channel Precision Temperature Controller for Hydrogen-Deuterium Exchange Mass Spectrometry Hudgens, Jeffrey W. J Res Natl Inst Stand Technol Article This tutorial provides mechanical drawings, electrical schematics, parts lists, stereolithography (STL) files for producing three-dimensional (3D)-printed parts, initial graphics exchange specification (IGS) files for automated machining, and instructions necessary for construction of a dual protease column, subzero, liquid chromatography system for hydrogen-deuterium exchange mass spectrometry (HDX-MS). Electro-mechanical schematics for construction of two multi-zone temperature controllers that regulate to ±0.05 oC are also included in this tutorial. [Gaithersburg, MD] : U.S. Dept. of Commerce, National Institute of Standards and Technology 2020-08-12 /pmc/articles/PMC9097885/ /pubmed/35573859 http://dx.doi.org/10.6028/jres.125.025 Text en https://creativecommons.org/publicdomain/zero/1.0/The Journal of Research of the National Institute of Standards and Technology is a publication of the U.S. Government. The papers are in the public domain and are not subject to copyright in the United States. Articles from J Res may contain photographs or illustrations copyrighted by other commercial organizations or individuals that may not be used without obtaining prior approval from the holder of the copyright.
spellingShingle Article
Hudgens, Jeffrey W.
Construction of a Dual Protease Column, Subzero (-30 °C) Chromatography System and Multi-channel Precision Temperature Controller for Hydrogen-Deuterium Exchange Mass Spectrometry
title Construction of a Dual Protease Column, Subzero (-30 °C) Chromatography System and Multi-channel Precision Temperature Controller for Hydrogen-Deuterium Exchange Mass Spectrometry
title_full Construction of a Dual Protease Column, Subzero (-30 °C) Chromatography System and Multi-channel Precision Temperature Controller for Hydrogen-Deuterium Exchange Mass Spectrometry
title_fullStr Construction of a Dual Protease Column, Subzero (-30 °C) Chromatography System and Multi-channel Precision Temperature Controller for Hydrogen-Deuterium Exchange Mass Spectrometry
title_full_unstemmed Construction of a Dual Protease Column, Subzero (-30 °C) Chromatography System and Multi-channel Precision Temperature Controller for Hydrogen-Deuterium Exchange Mass Spectrometry
title_short Construction of a Dual Protease Column, Subzero (-30 °C) Chromatography System and Multi-channel Precision Temperature Controller for Hydrogen-Deuterium Exchange Mass Spectrometry
title_sort construction of a dual protease column, subzero (-30 °c) chromatography system and multi-channel precision temperature controller for hydrogen-deuterium exchange mass spectrometry
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9097885/
https://www.ncbi.nlm.nih.gov/pubmed/35573859
http://dx.doi.org/10.6028/jres.125.025
work_keys_str_mv AT hudgensjeffreyw constructionofadualproteasecolumnsubzero30cchromatographysystemandmultichannelprecisiontemperaturecontrollerforhydrogendeuteriumexchangemassspectrometry

Ejemplares similares