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Microbiomes and Planctomycete diversity in large-scale aquaria habitats
In commercial large-scale aquaria, controlling levels of nitrogenous compounds is essential for macrofauna health. Naturally occurring bacteria are capable of transforming toxic nitrogen species into their more benign counterparts and play important roles in maintaining aquaria health. Nitrification...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9098025/ https://www.ncbi.nlm.nih.gov/pubmed/35551553 http://dx.doi.org/10.1371/journal.pone.0267881 |
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author | Elbon, Claire E. LeCleir, Gary R. Tuttle, Matthew J. Jurgensen, Sophie K. Demas, Thomas G. Keller, Christian J. Stewart, Tina Buchan, Alison |
author_facet | Elbon, Claire E. LeCleir, Gary R. Tuttle, Matthew J. Jurgensen, Sophie K. Demas, Thomas G. Keller, Christian J. Stewart, Tina Buchan, Alison |
author_sort | Elbon, Claire E. |
collection | PubMed |
description | In commercial large-scale aquaria, controlling levels of nitrogenous compounds is essential for macrofauna health. Naturally occurring bacteria are capable of transforming toxic nitrogen species into their more benign counterparts and play important roles in maintaining aquaria health. Nitrification, the microbially-mediated transformation of ammonium and nitrite to nitrate, is a common and encouraged process for management of both commercial and home aquaria. A potentially competing microbial process that transforms ammonium and nitrite to dinitrogen gas (anaerobic ammonium oxidation [anammox]) is mediated by some bacteria within the phylum Planctomycetes. Anammox has been harnessed for nitrogen removal during wastewater treatment, as the nitrogenous end product is released into the atmosphere rather than in aqueous discharge. Whether anammox bacteria could be similarly utilized in commercial aquaria is an open question. As a first step in assessing the viability of this practice, we (i) characterized microbial communities from water and sand filtration systems for four habitats at the Tennessee Aquarium and (ii) examined the abundance and anammox potential of Planctomycetes using culture-independent approaches. 16S rRNA gene amplicon sequencing revealed distinct, yet stable, microbial communities and the presence of Planctomycetes (~1–15% of library reads) in all sampled habitats. Preliminary metagenomic analyses identified the genetic potential for multiple complete nitrogen metabolism pathways. However, no known genes diagnostic for the anammox reaction were found in this survey. To better understand the diversity of this group of bacteria in these systems, a targeted Planctomycete-specific 16S rRNA gene-based PCR approach was used. This effort recovered amplicons that share <95% 16S rRNA gene sequence identity to previously characterized Planctomycetes, suggesting novel strains within this phylum reside within aquaria. |
format | Online Article Text |
id | pubmed-9098025 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-90980252022-05-13 Microbiomes and Planctomycete diversity in large-scale aquaria habitats Elbon, Claire E. LeCleir, Gary R. Tuttle, Matthew J. Jurgensen, Sophie K. Demas, Thomas G. Keller, Christian J. Stewart, Tina Buchan, Alison PLoS One Research Article In commercial large-scale aquaria, controlling levels of nitrogenous compounds is essential for macrofauna health. Naturally occurring bacteria are capable of transforming toxic nitrogen species into their more benign counterparts and play important roles in maintaining aquaria health. Nitrification, the microbially-mediated transformation of ammonium and nitrite to nitrate, is a common and encouraged process for management of both commercial and home aquaria. A potentially competing microbial process that transforms ammonium and nitrite to dinitrogen gas (anaerobic ammonium oxidation [anammox]) is mediated by some bacteria within the phylum Planctomycetes. Anammox has been harnessed for nitrogen removal during wastewater treatment, as the nitrogenous end product is released into the atmosphere rather than in aqueous discharge. Whether anammox bacteria could be similarly utilized in commercial aquaria is an open question. As a first step in assessing the viability of this practice, we (i) characterized microbial communities from water and sand filtration systems for four habitats at the Tennessee Aquarium and (ii) examined the abundance and anammox potential of Planctomycetes using culture-independent approaches. 16S rRNA gene amplicon sequencing revealed distinct, yet stable, microbial communities and the presence of Planctomycetes (~1–15% of library reads) in all sampled habitats. Preliminary metagenomic analyses identified the genetic potential for multiple complete nitrogen metabolism pathways. However, no known genes diagnostic for the anammox reaction were found in this survey. To better understand the diversity of this group of bacteria in these systems, a targeted Planctomycete-specific 16S rRNA gene-based PCR approach was used. This effort recovered amplicons that share <95% 16S rRNA gene sequence identity to previously characterized Planctomycetes, suggesting novel strains within this phylum reside within aquaria. Public Library of Science 2022-05-12 /pmc/articles/PMC9098025/ /pubmed/35551553 http://dx.doi.org/10.1371/journal.pone.0267881 Text en © 2022 Elbon et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Elbon, Claire E. LeCleir, Gary R. Tuttle, Matthew J. Jurgensen, Sophie K. Demas, Thomas G. Keller, Christian J. Stewart, Tina Buchan, Alison Microbiomes and Planctomycete diversity in large-scale aquaria habitats |
title | Microbiomes and Planctomycete diversity in large-scale aquaria habitats |
title_full | Microbiomes and Planctomycete diversity in large-scale aquaria habitats |
title_fullStr | Microbiomes and Planctomycete diversity in large-scale aquaria habitats |
title_full_unstemmed | Microbiomes and Planctomycete diversity in large-scale aquaria habitats |
title_short | Microbiomes and Planctomycete diversity in large-scale aquaria habitats |
title_sort | microbiomes and planctomycete diversity in large-scale aquaria habitats |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9098025/ https://www.ncbi.nlm.nih.gov/pubmed/35551553 http://dx.doi.org/10.1371/journal.pone.0267881 |
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