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Microbiomes and Planctomycete diversity in large-scale aquaria habitats

In commercial large-scale aquaria, controlling levels of nitrogenous compounds is essential for macrofauna health. Naturally occurring bacteria are capable of transforming toxic nitrogen species into their more benign counterparts and play important roles in maintaining aquaria health. Nitrification...

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Autores principales: Elbon, Claire E., LeCleir, Gary R., Tuttle, Matthew J., Jurgensen, Sophie K., Demas, Thomas G., Keller, Christian J., Stewart, Tina, Buchan, Alison
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9098025/
https://www.ncbi.nlm.nih.gov/pubmed/35551553
http://dx.doi.org/10.1371/journal.pone.0267881
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author Elbon, Claire E.
LeCleir, Gary R.
Tuttle, Matthew J.
Jurgensen, Sophie K.
Demas, Thomas G.
Keller, Christian J.
Stewart, Tina
Buchan, Alison
author_facet Elbon, Claire E.
LeCleir, Gary R.
Tuttle, Matthew J.
Jurgensen, Sophie K.
Demas, Thomas G.
Keller, Christian J.
Stewart, Tina
Buchan, Alison
author_sort Elbon, Claire E.
collection PubMed
description In commercial large-scale aquaria, controlling levels of nitrogenous compounds is essential for macrofauna health. Naturally occurring bacteria are capable of transforming toxic nitrogen species into their more benign counterparts and play important roles in maintaining aquaria health. Nitrification, the microbially-mediated transformation of ammonium and nitrite to nitrate, is a common and encouraged process for management of both commercial and home aquaria. A potentially competing microbial process that transforms ammonium and nitrite to dinitrogen gas (anaerobic ammonium oxidation [anammox]) is mediated by some bacteria within the phylum Planctomycetes. Anammox has been harnessed for nitrogen removal during wastewater treatment, as the nitrogenous end product is released into the atmosphere rather than in aqueous discharge. Whether anammox bacteria could be similarly utilized in commercial aquaria is an open question. As a first step in assessing the viability of this practice, we (i) characterized microbial communities from water and sand filtration systems for four habitats at the Tennessee Aquarium and (ii) examined the abundance and anammox potential of Planctomycetes using culture-independent approaches. 16S rRNA gene amplicon sequencing revealed distinct, yet stable, microbial communities and the presence of Planctomycetes (~1–15% of library reads) in all sampled habitats. Preliminary metagenomic analyses identified the genetic potential for multiple complete nitrogen metabolism pathways. However, no known genes diagnostic for the anammox reaction were found in this survey. To better understand the diversity of this group of bacteria in these systems, a targeted Planctomycete-specific 16S rRNA gene-based PCR approach was used. This effort recovered amplicons that share <95% 16S rRNA gene sequence identity to previously characterized Planctomycetes, suggesting novel strains within this phylum reside within aquaria.
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spelling pubmed-90980252022-05-13 Microbiomes and Planctomycete diversity in large-scale aquaria habitats Elbon, Claire E. LeCleir, Gary R. Tuttle, Matthew J. Jurgensen, Sophie K. Demas, Thomas G. Keller, Christian J. Stewart, Tina Buchan, Alison PLoS One Research Article In commercial large-scale aquaria, controlling levels of nitrogenous compounds is essential for macrofauna health. Naturally occurring bacteria are capable of transforming toxic nitrogen species into their more benign counterparts and play important roles in maintaining aquaria health. Nitrification, the microbially-mediated transformation of ammonium and nitrite to nitrate, is a common and encouraged process for management of both commercial and home aquaria. A potentially competing microbial process that transforms ammonium and nitrite to dinitrogen gas (anaerobic ammonium oxidation [anammox]) is mediated by some bacteria within the phylum Planctomycetes. Anammox has been harnessed for nitrogen removal during wastewater treatment, as the nitrogenous end product is released into the atmosphere rather than in aqueous discharge. Whether anammox bacteria could be similarly utilized in commercial aquaria is an open question. As a first step in assessing the viability of this practice, we (i) characterized microbial communities from water and sand filtration systems for four habitats at the Tennessee Aquarium and (ii) examined the abundance and anammox potential of Planctomycetes using culture-independent approaches. 16S rRNA gene amplicon sequencing revealed distinct, yet stable, microbial communities and the presence of Planctomycetes (~1–15% of library reads) in all sampled habitats. Preliminary metagenomic analyses identified the genetic potential for multiple complete nitrogen metabolism pathways. However, no known genes diagnostic for the anammox reaction were found in this survey. To better understand the diversity of this group of bacteria in these systems, a targeted Planctomycete-specific 16S rRNA gene-based PCR approach was used. This effort recovered amplicons that share <95% 16S rRNA gene sequence identity to previously characterized Planctomycetes, suggesting novel strains within this phylum reside within aquaria. Public Library of Science 2022-05-12 /pmc/articles/PMC9098025/ /pubmed/35551553 http://dx.doi.org/10.1371/journal.pone.0267881 Text en © 2022 Elbon et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Elbon, Claire E.
LeCleir, Gary R.
Tuttle, Matthew J.
Jurgensen, Sophie K.
Demas, Thomas G.
Keller, Christian J.
Stewart, Tina
Buchan, Alison
Microbiomes and Planctomycete diversity in large-scale aquaria habitats
title Microbiomes and Planctomycete diversity in large-scale aquaria habitats
title_full Microbiomes and Planctomycete diversity in large-scale aquaria habitats
title_fullStr Microbiomes and Planctomycete diversity in large-scale aquaria habitats
title_full_unstemmed Microbiomes and Planctomycete diversity in large-scale aquaria habitats
title_short Microbiomes and Planctomycete diversity in large-scale aquaria habitats
title_sort microbiomes and planctomycete diversity in large-scale aquaria habitats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9098025/
https://www.ncbi.nlm.nih.gov/pubmed/35551553
http://dx.doi.org/10.1371/journal.pone.0267881
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