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Exploration of the Molecular Mechanisms of Hyssopus cuspidatus Boriss Treatment of Asthma in an mRNA-miRNA Network via Bioinformatics Analysis

Hyssopus cuspidatus Boriss (H. cuspidatus) is a traditional Chinese medicine commonly used in the treatment of asthma. In the present study, we applied bioinformatics techniques for mRNA-miRNA profiling to elucidate the potential mechanisms of H. cuspidatus in asthma treatment. Bioactive compounds f...

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Autores principales: Cai, Zhongdi, Liu, Mimin, Yuan, Fengjuan, Zeng, Li, Zhao, Kaiyue, Sun, Ting, Li, Zhuorong, Liu, Rui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9098316/
https://www.ncbi.nlm.nih.gov/pubmed/35572723
http://dx.doi.org/10.1155/2022/7111901
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author Cai, Zhongdi
Liu, Mimin
Yuan, Fengjuan
Zeng, Li
Zhao, Kaiyue
Sun, Ting
Li, Zhuorong
Liu, Rui
author_facet Cai, Zhongdi
Liu, Mimin
Yuan, Fengjuan
Zeng, Li
Zhao, Kaiyue
Sun, Ting
Li, Zhuorong
Liu, Rui
author_sort Cai, Zhongdi
collection PubMed
description Hyssopus cuspidatus Boriss (H. cuspidatus) is a traditional Chinese medicine commonly used in the treatment of asthma. In the present study, we applied bioinformatics techniques for mRNA-miRNA profiling to elucidate the potential mechanisms of H. cuspidatus in asthma treatment. Bioactive compounds from H. cuspidatus, potential therapeutic targets of H. cuspidatus, and asthma-related targets were identified from the literature and databases. The intersection of H. cuspidatus-related targets and asthma-related targets was identified using the STRING platform. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed using the Metascape platform. Networks were constructed from these nodes using Cytoscape. The results showed that 23 active compounds were identified in H. cuspidatus, sharing 122 common asthma-related targets. Moreover, 43 miRNAs regulating 19 key targets involved in the antiasthmatic effects of H. cuspidatus were identified. Further analysis of biological pathways, active compound-key target-pathway network, and active compound-key target-miRNA network indicated that the antiasthmatic effects of H. cuspidatus mainly occurred through caffeic acid, methyl rosmarinate, luteolin, esculetin, and 8-hydroxycirsimaritin. These compounds interacted with multiple miRNAs, including miR-99a, miR-498, miR-33b, and miR-18a, regulating multiple genes, including JAK, STAT3, EGFR, LYN, and IL-6, in multiple pathways, including those involved in the regulation of JAK-STAT signaling, EGFR tyrosine kinase inhibitor resistance, PI3K-Akt signaling, and inflammation. In summary, we have elucidated the potential mechanisms of H. cuspidatus treatment of asthma from a systemic and holistic perspective through analysis of compound-mRNA-miRNA interaction. Our study should provide new insights for further research on H. cuspidatus treatment of asthma.
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spelling pubmed-90983162022-05-13 Exploration of the Molecular Mechanisms of Hyssopus cuspidatus Boriss Treatment of Asthma in an mRNA-miRNA Network via Bioinformatics Analysis Cai, Zhongdi Liu, Mimin Yuan, Fengjuan Zeng, Li Zhao, Kaiyue Sun, Ting Li, Zhuorong Liu, Rui Biomed Res Int Research Article Hyssopus cuspidatus Boriss (H. cuspidatus) is a traditional Chinese medicine commonly used in the treatment of asthma. In the present study, we applied bioinformatics techniques for mRNA-miRNA profiling to elucidate the potential mechanisms of H. cuspidatus in asthma treatment. Bioactive compounds from H. cuspidatus, potential therapeutic targets of H. cuspidatus, and asthma-related targets were identified from the literature and databases. The intersection of H. cuspidatus-related targets and asthma-related targets was identified using the STRING platform. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed using the Metascape platform. Networks were constructed from these nodes using Cytoscape. The results showed that 23 active compounds were identified in H. cuspidatus, sharing 122 common asthma-related targets. Moreover, 43 miRNAs regulating 19 key targets involved in the antiasthmatic effects of H. cuspidatus were identified. Further analysis of biological pathways, active compound-key target-pathway network, and active compound-key target-miRNA network indicated that the antiasthmatic effects of H. cuspidatus mainly occurred through caffeic acid, methyl rosmarinate, luteolin, esculetin, and 8-hydroxycirsimaritin. These compounds interacted with multiple miRNAs, including miR-99a, miR-498, miR-33b, and miR-18a, regulating multiple genes, including JAK, STAT3, EGFR, LYN, and IL-6, in multiple pathways, including those involved in the regulation of JAK-STAT signaling, EGFR tyrosine kinase inhibitor resistance, PI3K-Akt signaling, and inflammation. In summary, we have elucidated the potential mechanisms of H. cuspidatus treatment of asthma from a systemic and holistic perspective through analysis of compound-mRNA-miRNA interaction. Our study should provide new insights for further research on H. cuspidatus treatment of asthma. Hindawi 2022-05-05 /pmc/articles/PMC9098316/ /pubmed/35572723 http://dx.doi.org/10.1155/2022/7111901 Text en Copyright © 2022 Zhongdi Cai et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Cai, Zhongdi
Liu, Mimin
Yuan, Fengjuan
Zeng, Li
Zhao, Kaiyue
Sun, Ting
Li, Zhuorong
Liu, Rui
Exploration of the Molecular Mechanisms of Hyssopus cuspidatus Boriss Treatment of Asthma in an mRNA-miRNA Network via Bioinformatics Analysis
title Exploration of the Molecular Mechanisms of Hyssopus cuspidatus Boriss Treatment of Asthma in an mRNA-miRNA Network via Bioinformatics Analysis
title_full Exploration of the Molecular Mechanisms of Hyssopus cuspidatus Boriss Treatment of Asthma in an mRNA-miRNA Network via Bioinformatics Analysis
title_fullStr Exploration of the Molecular Mechanisms of Hyssopus cuspidatus Boriss Treatment of Asthma in an mRNA-miRNA Network via Bioinformatics Analysis
title_full_unstemmed Exploration of the Molecular Mechanisms of Hyssopus cuspidatus Boriss Treatment of Asthma in an mRNA-miRNA Network via Bioinformatics Analysis
title_short Exploration of the Molecular Mechanisms of Hyssopus cuspidatus Boriss Treatment of Asthma in an mRNA-miRNA Network via Bioinformatics Analysis
title_sort exploration of the molecular mechanisms of hyssopus cuspidatus boriss treatment of asthma in an mrna-mirna network via bioinformatics analysis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9098316/
https://www.ncbi.nlm.nih.gov/pubmed/35572723
http://dx.doi.org/10.1155/2022/7111901
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