Genome editing in animals with minimal PAM CRISPR-Cas9 enzymes

The requirement for Cas nucleases to recognize a specific PAM is a major restriction for genome editing. SpCas9 variants SpG and SpRY, recognizing NGN and NRN PAMs, respectively, have contributed to increase the number of editable genomic sites in cell cultures and plants. However, their use has not...

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Detalles Bibliográficos
Autores principales: Vicencio, Jeremy, Sánchez-Bolaños, Carlos, Moreno-Sánchez, Ismael, Brena, David, Vejnar, Charles E., Kukhtar, Dmytro, Ruiz-López, Miguel, Cots-Ponjoan, Mariona, Rubio, Alejandro, Melero, Natalia Rodrigo, Crespo-Cuadrado, Jesús, Carolis, Carlo, Pérez-Pulido, Antonio J., Giráldez, Antonio J., Kleinstiver, Benjamin P., Cerón, Julián, Moreno-Mateos, Miguel A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9098488/
https://www.ncbi.nlm.nih.gov/pubmed/35552388
http://dx.doi.org/10.1038/s41467-022-30228-4

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