Cargando…

Exogenous gonadotropin-releasing hormone counteracts the adverse effect of scrotal insulation on testicular functions in bucks

This study determined the effects of scrotal insulation on testicular functions in bucks and evaluated the impact of exogenous gonadotropin-releasing hormone (GnRH) administration before scrotal insulation on sperm production and testicular vascular dynamics. Twelve bucks were randomly divided into...

Descripción completa

Detalles Bibliográficos
Autores principales: Yousef, Mohamed S., Megahed, Gaber A., Abozed, Gamal F., Hayder, Mohamed, Abd-Elhafeez, Hanan H., Rawy, Mohamed S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9098548/
https://www.ncbi.nlm.nih.gov/pubmed/35551262
http://dx.doi.org/10.1038/s41598-022-11884-4
_version_ 1784706409611395072
author Yousef, Mohamed S.
Megahed, Gaber A.
Abozed, Gamal F.
Hayder, Mohamed
Abd-Elhafeez, Hanan H.
Rawy, Mohamed S.
author_facet Yousef, Mohamed S.
Megahed, Gaber A.
Abozed, Gamal F.
Hayder, Mohamed
Abd-Elhafeez, Hanan H.
Rawy, Mohamed S.
author_sort Yousef, Mohamed S.
collection PubMed
description This study determined the effects of scrotal insulation on testicular functions in bucks and evaluated the impact of exogenous gonadotropin-releasing hormone (GnRH) administration before scrotal insulation on sperm production and testicular vascular dynamics. Twelve bucks were randomly divided into three groups: scrotal-insulated animals without GnRH treatment (INS), scrotal-insulated animals treated previously with GnRH (GnRH + INS), and animals without insulation as controls (CON). Doppler ultrasonography was used to evaluate testicular vascular changes, and semen samples were collected to assess seminal parameters. Testicular samples were collected from slaughtered bucks at the end of the experiment for histological investigations and immunohistochemical analysis for caspase 3 (apoptotic marker), and a vascular endothelial growth factor (VEGF; hypoxic marker) evaluation. Sperm motility drastically decreased (33%) in the INS group on day 8 compared with those in the GnRH + INS and CON groups (58% and 85%, respectively). Testicular blood flow significantly decreased for 3 and 2 weeks in the INS and GnRH + INS groups, respectively. The pulsatility index (PI) reached pretreatment values at 5 and 4 weeks after insulation in the INS and GnRH + INS groups, respectively. The resistance index (RI) values increased in both insulated groups for the first 2 weeks and decreased to control values 4 weeks after insulation. However, the maximum velocity (VP) started to increase reaching pretreatment values by the 5th and 3rd weeks after insulation in the INS and GnRH + INS groups, respectively. Histological investigations showed a marked reduction in lipid inclusions in Sertoli cells in the GnRH + INS group compared with those in the INS group. The distributions of both caspase 3 and VEGF decreased in the GnRH + INS group compared with those in the INS group. This study showed that the administration of a single dose of GnRH delayed the negative effects of scrotal insulation on different seminal traits and revealed the pivotal role of GnRH in compensating testicular insulation in bucks.
format Online
Article
Text
id pubmed-9098548
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-90985482022-05-14 Exogenous gonadotropin-releasing hormone counteracts the adverse effect of scrotal insulation on testicular functions in bucks Yousef, Mohamed S. Megahed, Gaber A. Abozed, Gamal F. Hayder, Mohamed Abd-Elhafeez, Hanan H. Rawy, Mohamed S. Sci Rep Article This study determined the effects of scrotal insulation on testicular functions in bucks and evaluated the impact of exogenous gonadotropin-releasing hormone (GnRH) administration before scrotal insulation on sperm production and testicular vascular dynamics. Twelve bucks were randomly divided into three groups: scrotal-insulated animals without GnRH treatment (INS), scrotal-insulated animals treated previously with GnRH (GnRH + INS), and animals without insulation as controls (CON). Doppler ultrasonography was used to evaluate testicular vascular changes, and semen samples were collected to assess seminal parameters. Testicular samples were collected from slaughtered bucks at the end of the experiment for histological investigations and immunohistochemical analysis for caspase 3 (apoptotic marker), and a vascular endothelial growth factor (VEGF; hypoxic marker) evaluation. Sperm motility drastically decreased (33%) in the INS group on day 8 compared with those in the GnRH + INS and CON groups (58% and 85%, respectively). Testicular blood flow significantly decreased for 3 and 2 weeks in the INS and GnRH + INS groups, respectively. The pulsatility index (PI) reached pretreatment values at 5 and 4 weeks after insulation in the INS and GnRH + INS groups, respectively. The resistance index (RI) values increased in both insulated groups for the first 2 weeks and decreased to control values 4 weeks after insulation. However, the maximum velocity (VP) started to increase reaching pretreatment values by the 5th and 3rd weeks after insulation in the INS and GnRH + INS groups, respectively. Histological investigations showed a marked reduction in lipid inclusions in Sertoli cells in the GnRH + INS group compared with those in the INS group. The distributions of both caspase 3 and VEGF decreased in the GnRH + INS group compared with those in the INS group. This study showed that the administration of a single dose of GnRH delayed the negative effects of scrotal insulation on different seminal traits and revealed the pivotal role of GnRH in compensating testicular insulation in bucks. Nature Publishing Group UK 2022-05-12 /pmc/articles/PMC9098548/ /pubmed/35551262 http://dx.doi.org/10.1038/s41598-022-11884-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Yousef, Mohamed S.
Megahed, Gaber A.
Abozed, Gamal F.
Hayder, Mohamed
Abd-Elhafeez, Hanan H.
Rawy, Mohamed S.
Exogenous gonadotropin-releasing hormone counteracts the adverse effect of scrotal insulation on testicular functions in bucks
title Exogenous gonadotropin-releasing hormone counteracts the adverse effect of scrotal insulation on testicular functions in bucks
title_full Exogenous gonadotropin-releasing hormone counteracts the adverse effect of scrotal insulation on testicular functions in bucks
title_fullStr Exogenous gonadotropin-releasing hormone counteracts the adverse effect of scrotal insulation on testicular functions in bucks
title_full_unstemmed Exogenous gonadotropin-releasing hormone counteracts the adverse effect of scrotal insulation on testicular functions in bucks
title_short Exogenous gonadotropin-releasing hormone counteracts the adverse effect of scrotal insulation on testicular functions in bucks
title_sort exogenous gonadotropin-releasing hormone counteracts the adverse effect of scrotal insulation on testicular functions in bucks
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9098548/
https://www.ncbi.nlm.nih.gov/pubmed/35551262
http://dx.doi.org/10.1038/s41598-022-11884-4
work_keys_str_mv AT yousefmohameds exogenousgonadotropinreleasinghormonecounteractstheadverseeffectofscrotalinsulationontesticularfunctionsinbucks
AT megahedgabera exogenousgonadotropinreleasinghormonecounteractstheadverseeffectofscrotalinsulationontesticularfunctionsinbucks
AT abozedgamalf exogenousgonadotropinreleasinghormonecounteractstheadverseeffectofscrotalinsulationontesticularfunctionsinbucks
AT haydermohamed exogenousgonadotropinreleasinghormonecounteractstheadverseeffectofscrotalinsulationontesticularfunctionsinbucks
AT abdelhafeezhananh exogenousgonadotropinreleasinghormonecounteractstheadverseeffectofscrotalinsulationontesticularfunctionsinbucks
AT rawymohameds exogenousgonadotropinreleasinghormonecounteractstheadverseeffectofscrotalinsulationontesticularfunctionsinbucks