Cargando…
Circulating MicroRNA-505 May Serve as a Prognostic Biomarker for Hypertension-Associated Endothelial Dysfunction and Inflammation
Our previous study has reported that the plasma microRNA-505 (miR-505) is elevated in hypertensive patients. However, the pathophysiological significance of miR-505 in hypertension remains to be elucidated. Hypertension is not only a vascular disorder, but also an inflammatory condition. The current...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9099007/ https://www.ncbi.nlm.nih.gov/pubmed/35571179 http://dx.doi.org/10.3389/fcvm.2022.834121 |
_version_ | 1784706507824168960 |
---|---|
author | Yang, Qinbo Wang, Peiwei Cai, Yiqing Cui, Yimeng Cui, Jingang Du, Xiaoye Chen, Yu Zhang, Teng |
author_facet | Yang, Qinbo Wang, Peiwei Cai, Yiqing Cui, Yimeng Cui, Jingang Du, Xiaoye Chen, Yu Zhang, Teng |
author_sort | Yang, Qinbo |
collection | PubMed |
description | Our previous study has reported that the plasma microRNA-505 (miR-505) is elevated in hypertensive patients. However, the pathophysiological significance of miR-505 in hypertension remains to be elucidated. Hypertension is not only a vascular disorder, but also an inflammatory condition. The current study therefore aims to further investigate the pathophysiological implications of miR-505 in hypertension-associated vascular and inflammatory changes. In vivo experiments reveal that the plasma level of miR-505 is elevated in spontaneously hypertensive rats and angiotensin II-infused mice. In addition, miR-505 agomir treatment results in elevated blood pressure, endothelial dysfunction, increased vascular expression of inflammatory genes and renal inflammatory injuries as well as pre-activation of PBMCs in mice. In vitro experiments further demonstrate that miR-505 agomir increases the expression of IL1B and TNFA, whereas miR-505 antagomir attenuates TNF-α-induced upregulation of IL1B and TNFA in endothelial cells, HUVECs. In addition, miR-505 modulates the levels of endothelial activation markers VCAM1 and E-selectin in HUVECs as well as the adhesion of THP-1 monocytes to HUVECs. Lastly, the plasma level of miR-505 is positively correlated with systolic blood pressure and the level of C-reactive protein in human subjects. Our work links for the first time miR-505 to endothelial dysfunction and inflammation under hypertensive conditions, supporting the translational value of miR-505 in prognosticating hypertension-associated endothelial impairment and inflammatory injuries in target organs such as the vessels and kidneys. |
format | Online Article Text |
id | pubmed-9099007 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-90990072022-05-14 Circulating MicroRNA-505 May Serve as a Prognostic Biomarker for Hypertension-Associated Endothelial Dysfunction and Inflammation Yang, Qinbo Wang, Peiwei Cai, Yiqing Cui, Yimeng Cui, Jingang Du, Xiaoye Chen, Yu Zhang, Teng Front Cardiovasc Med Cardiovascular Medicine Our previous study has reported that the plasma microRNA-505 (miR-505) is elevated in hypertensive patients. However, the pathophysiological significance of miR-505 in hypertension remains to be elucidated. Hypertension is not only a vascular disorder, but also an inflammatory condition. The current study therefore aims to further investigate the pathophysiological implications of miR-505 in hypertension-associated vascular and inflammatory changes. In vivo experiments reveal that the plasma level of miR-505 is elevated in spontaneously hypertensive rats and angiotensin II-infused mice. In addition, miR-505 agomir treatment results in elevated blood pressure, endothelial dysfunction, increased vascular expression of inflammatory genes and renal inflammatory injuries as well as pre-activation of PBMCs in mice. In vitro experiments further demonstrate that miR-505 agomir increases the expression of IL1B and TNFA, whereas miR-505 antagomir attenuates TNF-α-induced upregulation of IL1B and TNFA in endothelial cells, HUVECs. In addition, miR-505 modulates the levels of endothelial activation markers VCAM1 and E-selectin in HUVECs as well as the adhesion of THP-1 monocytes to HUVECs. Lastly, the plasma level of miR-505 is positively correlated with systolic blood pressure and the level of C-reactive protein in human subjects. Our work links for the first time miR-505 to endothelial dysfunction and inflammation under hypertensive conditions, supporting the translational value of miR-505 in prognosticating hypertension-associated endothelial impairment and inflammatory injuries in target organs such as the vessels and kidneys. Frontiers Media S.A. 2022-04-29 /pmc/articles/PMC9099007/ /pubmed/35571179 http://dx.doi.org/10.3389/fcvm.2022.834121 Text en Copyright © 2022 Yang, Wang, Cai, Cui, Cui, Du, Chen and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cardiovascular Medicine Yang, Qinbo Wang, Peiwei Cai, Yiqing Cui, Yimeng Cui, Jingang Du, Xiaoye Chen, Yu Zhang, Teng Circulating MicroRNA-505 May Serve as a Prognostic Biomarker for Hypertension-Associated Endothelial Dysfunction and Inflammation |
title | Circulating MicroRNA-505 May Serve as a Prognostic Biomarker for Hypertension-Associated Endothelial Dysfunction and Inflammation |
title_full | Circulating MicroRNA-505 May Serve as a Prognostic Biomarker for Hypertension-Associated Endothelial Dysfunction and Inflammation |
title_fullStr | Circulating MicroRNA-505 May Serve as a Prognostic Biomarker for Hypertension-Associated Endothelial Dysfunction and Inflammation |
title_full_unstemmed | Circulating MicroRNA-505 May Serve as a Prognostic Biomarker for Hypertension-Associated Endothelial Dysfunction and Inflammation |
title_short | Circulating MicroRNA-505 May Serve as a Prognostic Biomarker for Hypertension-Associated Endothelial Dysfunction and Inflammation |
title_sort | circulating microrna-505 may serve as a prognostic biomarker for hypertension-associated endothelial dysfunction and inflammation |
topic | Cardiovascular Medicine |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9099007/ https://www.ncbi.nlm.nih.gov/pubmed/35571179 http://dx.doi.org/10.3389/fcvm.2022.834121 |
work_keys_str_mv | AT yangqinbo circulatingmicrorna505mayserveasaprognosticbiomarkerforhypertensionassociatedendothelialdysfunctionandinflammation AT wangpeiwei circulatingmicrorna505mayserveasaprognosticbiomarkerforhypertensionassociatedendothelialdysfunctionandinflammation AT caiyiqing circulatingmicrorna505mayserveasaprognosticbiomarkerforhypertensionassociatedendothelialdysfunctionandinflammation AT cuiyimeng circulatingmicrorna505mayserveasaprognosticbiomarkerforhypertensionassociatedendothelialdysfunctionandinflammation AT cuijingang circulatingmicrorna505mayserveasaprognosticbiomarkerforhypertensionassociatedendothelialdysfunctionandinflammation AT duxiaoye circulatingmicrorna505mayserveasaprognosticbiomarkerforhypertensionassociatedendothelialdysfunctionandinflammation AT chenyu circulatingmicrorna505mayserveasaprognosticbiomarkerforhypertensionassociatedendothelialdysfunctionandinflammation AT zhangteng circulatingmicrorna505mayserveasaprognosticbiomarkerforhypertensionassociatedendothelialdysfunctionandinflammation |