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An endpoint visualization loop-mediated isothermal amplification (LAMP) for detecting bubaline theileriosis

BACKGROUND: Tropical theileriosis is a significant disease affecting the health and production levels of buffaloes in India. It is caused by an apicomplexan—Theileria annulata. The timely and accurate detection of infection is vital for implementing a mass vaccination or control programme in a given...

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Autores principales: Kumar, Sanjeev, Paliwal, Sanjhi, Sudan, Vikrant, Shanker, Daya, Singh, Shanker Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9099057/
https://www.ncbi.nlm.nih.gov/pubmed/35582477
http://dx.doi.org/10.1186/s43088-022-00251-x
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author Kumar, Sanjeev
Paliwal, Sanjhi
Sudan, Vikrant
Shanker, Daya
Singh, Shanker Kumar
author_facet Kumar, Sanjeev
Paliwal, Sanjhi
Sudan, Vikrant
Shanker, Daya
Singh, Shanker Kumar
author_sort Kumar, Sanjeev
collection PubMed
description BACKGROUND: Tropical theileriosis is a significant disease affecting the health and production levels of buffaloes in India. It is caused by an apicomplexan—Theileria annulata. The timely and accurate detection of infection is vital for implementing a mass vaccination or control programme in a given area under outbreak. Most of the literature concerned with diagnosis of theileriosis revolves around cattle, and practically, there are very limited assays available for detecting bubaline theileriosis. Loop-mediated isothermal amplification (LAMP) assay certainly amplifies the targeted deoxyribosenucleic acid (DNA) with a comparatively higher efficacy, rapidity and sensitivity. Alongside, minimal use of sophisticated instruments in performing LAMP assay is certainly an add on. The present study describes the application of LAMP assay in diagnosing tropical theileriosis in buffaloes alongside, its comparison with polymerase chain reaction (PCR) and blood microscopical examination. RESULTS: No cross-reaction was seen with DNA of other haemoprotozoan. LAMP was compared with blood microscopy and PCR. LAMP detected infection in 27 out of 100 buffaloes, while blood microscopy and PCR detected disease in 16 and 24 buffaloes, respectively. CONCLUSION: The sensitivity, specificity and kappa value prediction of LAMP were found to be much higher than the PCR and blood microscopy. The present communication reports the first use of LAMP in detecting theileriosis in buffaloes in the world.
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spelling pubmed-90990572022-05-13 An endpoint visualization loop-mediated isothermal amplification (LAMP) for detecting bubaline theileriosis Kumar, Sanjeev Paliwal, Sanjhi Sudan, Vikrant Shanker, Daya Singh, Shanker Kumar Beni Suef Univ J Basic Appl Sci Research BACKGROUND: Tropical theileriosis is a significant disease affecting the health and production levels of buffaloes in India. It is caused by an apicomplexan—Theileria annulata. The timely and accurate detection of infection is vital for implementing a mass vaccination or control programme in a given area under outbreak. Most of the literature concerned with diagnosis of theileriosis revolves around cattle, and practically, there are very limited assays available for detecting bubaline theileriosis. Loop-mediated isothermal amplification (LAMP) assay certainly amplifies the targeted deoxyribosenucleic acid (DNA) with a comparatively higher efficacy, rapidity and sensitivity. Alongside, minimal use of sophisticated instruments in performing LAMP assay is certainly an add on. The present study describes the application of LAMP assay in diagnosing tropical theileriosis in buffaloes alongside, its comparison with polymerase chain reaction (PCR) and blood microscopical examination. RESULTS: No cross-reaction was seen with DNA of other haemoprotozoan. LAMP was compared with blood microscopy and PCR. LAMP detected infection in 27 out of 100 buffaloes, while blood microscopy and PCR detected disease in 16 and 24 buffaloes, respectively. CONCLUSION: The sensitivity, specificity and kappa value prediction of LAMP were found to be much higher than the PCR and blood microscopy. The present communication reports the first use of LAMP in detecting theileriosis in buffaloes in the world. Springer Berlin Heidelberg 2022-05-13 2022 /pmc/articles/PMC9099057/ /pubmed/35582477 http://dx.doi.org/10.1186/s43088-022-00251-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research
Kumar, Sanjeev
Paliwal, Sanjhi
Sudan, Vikrant
Shanker, Daya
Singh, Shanker Kumar
An endpoint visualization loop-mediated isothermal amplification (LAMP) for detecting bubaline theileriosis
title An endpoint visualization loop-mediated isothermal amplification (LAMP) for detecting bubaline theileriosis
title_full An endpoint visualization loop-mediated isothermal amplification (LAMP) for detecting bubaline theileriosis
title_fullStr An endpoint visualization loop-mediated isothermal amplification (LAMP) for detecting bubaline theileriosis
title_full_unstemmed An endpoint visualization loop-mediated isothermal amplification (LAMP) for detecting bubaline theileriosis
title_short An endpoint visualization loop-mediated isothermal amplification (LAMP) for detecting bubaline theileriosis
title_sort endpoint visualization loop-mediated isothermal amplification (lamp) for detecting bubaline theileriosis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9099057/
https://www.ncbi.nlm.nih.gov/pubmed/35582477
http://dx.doi.org/10.1186/s43088-022-00251-x
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