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The Biodiversity of Grapevine Bacterial Endophytes of Vitis amurensis Rupr.

In this paper, the composition profiles of bacterial endophytes in wild-growing Amur grape Vitis amurensis Rupr. grown in the south of the Russian Far East were analyzed using both a cultivation-dependent (sowing bacteria) and a cultivation-independent (next generation sequencing, NGS) approach. Bot...

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Autores principales: Aleynova, Olga A., Nityagovsky, Nikolay N., Dubrovina, Alexandra S., Kiselev, Konstantin V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9099740/
https://www.ncbi.nlm.nih.gov/pubmed/35567129
http://dx.doi.org/10.3390/plants11091128
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author Aleynova, Olga A.
Nityagovsky, Nikolay N.
Dubrovina, Alexandra S.
Kiselev, Konstantin V.
author_facet Aleynova, Olga A.
Nityagovsky, Nikolay N.
Dubrovina, Alexandra S.
Kiselev, Konstantin V.
author_sort Aleynova, Olga A.
collection PubMed
description In this paper, the composition profiles of bacterial endophytes in wild-growing Amur grape Vitis amurensis Rupr. grown in the south of the Russian Far East were analyzed using both a cultivation-dependent (sowing bacteria) and a cultivation-independent (next generation sequencing, NGS) approach. Both methods revealed the prevalent endophytes in V. amurensis were represented by Gammaproteobacteria—40.3–75.8%, Alphaproteobacteria—8.6–18.7%, Actinobacteria—9.2–15.4%, and Bacilli—6.1–6.6%. NGS also showed a large proportion of Bacteroidia (12.2%) and a small proportion of other classes (less than 5.7%). In general, NGS revealed a greater variety of classes and genera in the endophytic bacterial community due to a high number of reads (574,207) in comparison with the number of colonies (933) obtained after the cultivation-dependent method. A comparative analysis performed in this study showed that both wild grape V. amurensis from Russia and domesticated cultivars of V. vinifera from Germany and California (USA) exhibit the same basic composition of endophytic bacteria, while the percentages of major taxa and minor taxa showed some differences depending on the plant organ, grape individuals, environmental conditions, and sampling time. Furthermore, the obtained data revealed that lower temperatures and increased precipitation favored the number and diversity of endophytic bacteria in the wild Amur grape. Thus, this study firstly described and analyzed the biodiversity of endophytic bacteria in wild grapevine V. amurensis.
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spelling pubmed-90997402022-05-14 The Biodiversity of Grapevine Bacterial Endophytes of Vitis amurensis Rupr. Aleynova, Olga A. Nityagovsky, Nikolay N. Dubrovina, Alexandra S. Kiselev, Konstantin V. Plants (Basel) Article In this paper, the composition profiles of bacterial endophytes in wild-growing Amur grape Vitis amurensis Rupr. grown in the south of the Russian Far East were analyzed using both a cultivation-dependent (sowing bacteria) and a cultivation-independent (next generation sequencing, NGS) approach. Both methods revealed the prevalent endophytes in V. amurensis were represented by Gammaproteobacteria—40.3–75.8%, Alphaproteobacteria—8.6–18.7%, Actinobacteria—9.2–15.4%, and Bacilli—6.1–6.6%. NGS also showed a large proportion of Bacteroidia (12.2%) and a small proportion of other classes (less than 5.7%). In general, NGS revealed a greater variety of classes and genera in the endophytic bacterial community due to a high number of reads (574,207) in comparison with the number of colonies (933) obtained after the cultivation-dependent method. A comparative analysis performed in this study showed that both wild grape V. amurensis from Russia and domesticated cultivars of V. vinifera from Germany and California (USA) exhibit the same basic composition of endophytic bacteria, while the percentages of major taxa and minor taxa showed some differences depending on the plant organ, grape individuals, environmental conditions, and sampling time. Furthermore, the obtained data revealed that lower temperatures and increased precipitation favored the number and diversity of endophytic bacteria in the wild Amur grape. Thus, this study firstly described and analyzed the biodiversity of endophytic bacteria in wild grapevine V. amurensis. MDPI 2022-04-21 /pmc/articles/PMC9099740/ /pubmed/35567129 http://dx.doi.org/10.3390/plants11091128 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Aleynova, Olga A.
Nityagovsky, Nikolay N.
Dubrovina, Alexandra S.
Kiselev, Konstantin V.
The Biodiversity of Grapevine Bacterial Endophytes of Vitis amurensis Rupr.
title The Biodiversity of Grapevine Bacterial Endophytes of Vitis amurensis Rupr.
title_full The Biodiversity of Grapevine Bacterial Endophytes of Vitis amurensis Rupr.
title_fullStr The Biodiversity of Grapevine Bacterial Endophytes of Vitis amurensis Rupr.
title_full_unstemmed The Biodiversity of Grapevine Bacterial Endophytes of Vitis amurensis Rupr.
title_short The Biodiversity of Grapevine Bacterial Endophytes of Vitis amurensis Rupr.
title_sort biodiversity of grapevine bacterial endophytes of vitis amurensis rupr.
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9099740/
https://www.ncbi.nlm.nih.gov/pubmed/35567129
http://dx.doi.org/10.3390/plants11091128
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