Cargando…
Isolation of Potato Endophytes and Screening of Chaetomium globosum Antimicrobial Genes
Antimicrobial peptides (AMPs) have natural antibacterial activities that pathogens find difficult to overcome. As a result of this occurrence, AMPs can act as an important substitute against the microbial resistance. In this study, we used plate confrontation tests to screen out 20 potential endophy...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9099842/ https://www.ncbi.nlm.nih.gov/pubmed/35563004 http://dx.doi.org/10.3390/ijms23094611 |
_version_ | 1784706706303877120 |
---|---|
author | Zhang, Jiaxin Islam, Md. Samiul Wang, Jieyu Zhao, Yang Dong, Wubei |
author_facet | Zhang, Jiaxin Islam, Md. Samiul Wang, Jieyu Zhao, Yang Dong, Wubei |
author_sort | Zhang, Jiaxin |
collection | PubMed |
description | Antimicrobial peptides (AMPs) have natural antibacterial activities that pathogens find difficult to overcome. As a result of this occurrence, AMPs can act as an important substitute against the microbial resistance. In this study, we used plate confrontation tests to screen out 20 potential endophytes from potato tubers. Among them, endophyte F5 was found to significantly inhibit the growth of five different pathogenic fungi. Following that, phylogenetic analysis revealed that the internal transcribed spacer (ITS) sequences were 99% identical to Chaetomium globosum corresponding sequences. Thereafter, the Bacillus subtilis expression system was used to create a C. globosum cDNA library in order to isolate the resistance genes. Using this approach, the resistance gene screening technology in the indicator bacteria built-in library was used to identify two antimicrobial peptides, CgR2150 and CgR3101, with broad-spectrum antibacterial activities. Furthermore, the results showed that CgR2150 and CgR3101 have excellent UV, thermal, and enzyme stabilities. Also, these two peptides can significantly inhibit the growth of various bacteria (Xanthomonas oryzae pv. oryzae, Xanthomonas oryzae pv. oryzicola, Clavibacter michiganensis, and Clavibacter fangii) and fungi (Fusarium graminearum, Rhizoctonia solani, and Botrytis cinerea). Scanning electron microscopy (SEM) observations revealed that CgR2150 and CgR3101 peptides act against bacteria by disrupting bacterial cell membranes. Moreover, hemolytic activity assay showed that neither of the two peptides exhibited significant hemolytic activity. To conclude, the antimicrobial peptides CgR2150 and CgR3101 are promising in the development of a new antibacterial agent and for application in plant production. |
format | Online Article Text |
id | pubmed-9099842 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-90998422022-05-14 Isolation of Potato Endophytes and Screening of Chaetomium globosum Antimicrobial Genes Zhang, Jiaxin Islam, Md. Samiul Wang, Jieyu Zhao, Yang Dong, Wubei Int J Mol Sci Article Antimicrobial peptides (AMPs) have natural antibacterial activities that pathogens find difficult to overcome. As a result of this occurrence, AMPs can act as an important substitute against the microbial resistance. In this study, we used plate confrontation tests to screen out 20 potential endophytes from potato tubers. Among them, endophyte F5 was found to significantly inhibit the growth of five different pathogenic fungi. Following that, phylogenetic analysis revealed that the internal transcribed spacer (ITS) sequences were 99% identical to Chaetomium globosum corresponding sequences. Thereafter, the Bacillus subtilis expression system was used to create a C. globosum cDNA library in order to isolate the resistance genes. Using this approach, the resistance gene screening technology in the indicator bacteria built-in library was used to identify two antimicrobial peptides, CgR2150 and CgR3101, with broad-spectrum antibacterial activities. Furthermore, the results showed that CgR2150 and CgR3101 have excellent UV, thermal, and enzyme stabilities. Also, these two peptides can significantly inhibit the growth of various bacteria (Xanthomonas oryzae pv. oryzae, Xanthomonas oryzae pv. oryzicola, Clavibacter michiganensis, and Clavibacter fangii) and fungi (Fusarium graminearum, Rhizoctonia solani, and Botrytis cinerea). Scanning electron microscopy (SEM) observations revealed that CgR2150 and CgR3101 peptides act against bacteria by disrupting bacterial cell membranes. Moreover, hemolytic activity assay showed that neither of the two peptides exhibited significant hemolytic activity. To conclude, the antimicrobial peptides CgR2150 and CgR3101 are promising in the development of a new antibacterial agent and for application in plant production. MDPI 2022-04-21 /pmc/articles/PMC9099842/ /pubmed/35563004 http://dx.doi.org/10.3390/ijms23094611 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhang, Jiaxin Islam, Md. Samiul Wang, Jieyu Zhao, Yang Dong, Wubei Isolation of Potato Endophytes and Screening of Chaetomium globosum Antimicrobial Genes |
title | Isolation of Potato Endophytes and Screening of Chaetomium globosum Antimicrobial Genes |
title_full | Isolation of Potato Endophytes and Screening of Chaetomium globosum Antimicrobial Genes |
title_fullStr | Isolation of Potato Endophytes and Screening of Chaetomium globosum Antimicrobial Genes |
title_full_unstemmed | Isolation of Potato Endophytes and Screening of Chaetomium globosum Antimicrobial Genes |
title_short | Isolation of Potato Endophytes and Screening of Chaetomium globosum Antimicrobial Genes |
title_sort | isolation of potato endophytes and screening of chaetomium globosum antimicrobial genes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9099842/ https://www.ncbi.nlm.nih.gov/pubmed/35563004 http://dx.doi.org/10.3390/ijms23094611 |
work_keys_str_mv | AT zhangjiaxin isolationofpotatoendophytesandscreeningofchaetomiumglobosumantimicrobialgenes AT islammdsamiul isolationofpotatoendophytesandscreeningofchaetomiumglobosumantimicrobialgenes AT wangjieyu isolationofpotatoendophytesandscreeningofchaetomiumglobosumantimicrobialgenes AT zhaoyang isolationofpotatoendophytesandscreeningofchaetomiumglobosumantimicrobialgenes AT dongwubei isolationofpotatoendophytesandscreeningofchaetomiumglobosumantimicrobialgenes |