Cargando…
Identification of p-Coumaric Acid and Ethyl p-Coumarate as the Main Phenolic Components of Hemp (Cannabis sativa L.) Roots
Hemp (Cannabis sativa L.) contains a variety of secondary metabolites, including cannabinoids, such as psychoactive (−)-trans-Δ⁹-tetrahydrocannabinol. The present study was conducted to identify the major phenolic components contained in hemp root, which has been relatively under-researched compared...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100028/ https://www.ncbi.nlm.nih.gov/pubmed/35566134 http://dx.doi.org/10.3390/molecules27092781 |
_version_ | 1784706752637304832 |
---|---|
author | Oh, Chang Min Choi, Joon Yong Bae, In Ah Kim, Hong Taek Hong, Seong Su Noah, Jay Kyun Boo, Yong Chool |
author_facet | Oh, Chang Min Choi, Joon Yong Bae, In Ah Kim, Hong Taek Hong, Seong Su Noah, Jay Kyun Boo, Yong Chool |
author_sort | Oh, Chang Min |
collection | PubMed |
description | Hemp (Cannabis sativa L.) contains a variety of secondary metabolites, including cannabinoids, such as psychoactive (−)-trans-Δ⁹-tetrahydrocannabinol. The present study was conducted to identify the major phenolic components contained in hemp root, which has been relatively under-researched compared to other parts of hemp. The aqueous ethanol extract of hemp roots was fractionated into methylene chloride (MC), ethyl acetate (EA), and water (WT) fractions, and high-performance liquid chromatography with photodiode array detection (HPLC-DAD) analysis was performed. The main ultraviolet (UV)-absorbing phenolic compound contained in the EA fraction was identified as p-coumaric acid by comparing the retention time and UV absorption spectrum with a standard. Silica gel column chromatography was performed to isolate a hydrophobic derivative of p-coumaric acid contained in the MC fraction. Nuclear magnetic resonance (NMR) analysis identified the isolated compound as ethyl p-coumarate. For comparative purposes, ethyl p-coumarate was also chemically synthesized by the esterification reaction of p-coumaric acid. The content of p-coumaric acid and ethyl p-coumarate in the total extract of hemp root was estimated to be 2.61 mg g(−1) and 6.47 mg g(−1), respectively, by HPLC-DAD analysis. These values correspond to 84 mg Kg(−1) dry root and 216 mg Kg(−)(1) dry root, respectively. In conclusion, this study identified p-coumaric acid and ethyl p-coumarate as the main phenolic compounds contained in the hemp roots. |
format | Online Article Text |
id | pubmed-9100028 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91000282022-05-14 Identification of p-Coumaric Acid and Ethyl p-Coumarate as the Main Phenolic Components of Hemp (Cannabis sativa L.) Roots Oh, Chang Min Choi, Joon Yong Bae, In Ah Kim, Hong Taek Hong, Seong Su Noah, Jay Kyun Boo, Yong Chool Molecules Communication Hemp (Cannabis sativa L.) contains a variety of secondary metabolites, including cannabinoids, such as psychoactive (−)-trans-Δ⁹-tetrahydrocannabinol. The present study was conducted to identify the major phenolic components contained in hemp root, which has been relatively under-researched compared to other parts of hemp. The aqueous ethanol extract of hemp roots was fractionated into methylene chloride (MC), ethyl acetate (EA), and water (WT) fractions, and high-performance liquid chromatography with photodiode array detection (HPLC-DAD) analysis was performed. The main ultraviolet (UV)-absorbing phenolic compound contained in the EA fraction was identified as p-coumaric acid by comparing the retention time and UV absorption spectrum with a standard. Silica gel column chromatography was performed to isolate a hydrophobic derivative of p-coumaric acid contained in the MC fraction. Nuclear magnetic resonance (NMR) analysis identified the isolated compound as ethyl p-coumarate. For comparative purposes, ethyl p-coumarate was also chemically synthesized by the esterification reaction of p-coumaric acid. The content of p-coumaric acid and ethyl p-coumarate in the total extract of hemp root was estimated to be 2.61 mg g(−1) and 6.47 mg g(−1), respectively, by HPLC-DAD analysis. These values correspond to 84 mg Kg(−1) dry root and 216 mg Kg(−)(1) dry root, respectively. In conclusion, this study identified p-coumaric acid and ethyl p-coumarate as the main phenolic compounds contained in the hemp roots. MDPI 2022-04-27 /pmc/articles/PMC9100028/ /pubmed/35566134 http://dx.doi.org/10.3390/molecules27092781 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Oh, Chang Min Choi, Joon Yong Bae, In Ah Kim, Hong Taek Hong, Seong Su Noah, Jay Kyun Boo, Yong Chool Identification of p-Coumaric Acid and Ethyl p-Coumarate as the Main Phenolic Components of Hemp (Cannabis sativa L.) Roots |
title | Identification of p-Coumaric Acid and Ethyl p-Coumarate as the Main Phenolic Components of Hemp (Cannabis sativa L.) Roots |
title_full | Identification of p-Coumaric Acid and Ethyl p-Coumarate as the Main Phenolic Components of Hemp (Cannabis sativa L.) Roots |
title_fullStr | Identification of p-Coumaric Acid and Ethyl p-Coumarate as the Main Phenolic Components of Hemp (Cannabis sativa L.) Roots |
title_full_unstemmed | Identification of p-Coumaric Acid and Ethyl p-Coumarate as the Main Phenolic Components of Hemp (Cannabis sativa L.) Roots |
title_short | Identification of p-Coumaric Acid and Ethyl p-Coumarate as the Main Phenolic Components of Hemp (Cannabis sativa L.) Roots |
title_sort | identification of p-coumaric acid and ethyl p-coumarate as the main phenolic components of hemp (cannabis sativa l.) roots |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100028/ https://www.ncbi.nlm.nih.gov/pubmed/35566134 http://dx.doi.org/10.3390/molecules27092781 |
work_keys_str_mv | AT ohchangmin identificationofpcoumaricacidandethylpcoumarateasthemainphenoliccomponentsofhempcannabissativalroots AT choijoonyong identificationofpcoumaricacidandethylpcoumarateasthemainphenoliccomponentsofhempcannabissativalroots AT baeinah identificationofpcoumaricacidandethylpcoumarateasthemainphenoliccomponentsofhempcannabissativalroots AT kimhongtaek identificationofpcoumaricacidandethylpcoumarateasthemainphenoliccomponentsofhempcannabissativalroots AT hongseongsu identificationofpcoumaricacidandethylpcoumarateasthemainphenoliccomponentsofhempcannabissativalroots AT noahjaykyun identificationofpcoumaricacidandethylpcoumarateasthemainphenoliccomponentsofhempcannabissativalroots AT booyongchool identificationofpcoumaricacidandethylpcoumarateasthemainphenoliccomponentsofhempcannabissativalroots |