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Lung Extracellular Matrix Hydrogels Enhance Preservation of Type II Phenotype in Primary Alveolar Epithelial Cells

One of the main limitations of in vitro studies on lung diseases is the difficulty of maintaining the type II phenotype of alveolar epithelial cells in culture. This fact has previously been related to the translocation of the mechanosensing Yes-associated protein (YAP) to the nuclei and Rho signali...

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Autores principales: Marhuenda, Esther, Villarino, Alvaro, Narciso, Maria Leonor, Camprubí-Rimblas, Marta, Farré, Ramon, Gavara, Núria, Artigas, Antonio, Almendros, Isaac, Otero, Jorge
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100165/
https://www.ncbi.nlm.nih.gov/pubmed/35563279
http://dx.doi.org/10.3390/ijms23094888
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author Marhuenda, Esther
Villarino, Alvaro
Narciso, Maria Leonor
Camprubí-Rimblas, Marta
Farré, Ramon
Gavara, Núria
Artigas, Antonio
Almendros, Isaac
Otero, Jorge
author_facet Marhuenda, Esther
Villarino, Alvaro
Narciso, Maria Leonor
Camprubí-Rimblas, Marta
Farré, Ramon
Gavara, Núria
Artigas, Antonio
Almendros, Isaac
Otero, Jorge
author_sort Marhuenda, Esther
collection PubMed
description One of the main limitations of in vitro studies on lung diseases is the difficulty of maintaining the type II phenotype of alveolar epithelial cells in culture. This fact has previously been related to the translocation of the mechanosensing Yes-associated protein (YAP) to the nuclei and Rho signaling pathway. In this work, we aimed to culture and subculture primary alveolar type II cells on extracellular matrix lung-derived hydrogels to assess their suitability for phenotype maintenance. Cells cultured on lung hydrogels formed monolayers and maintained type II phenotype for a longer time as compared with those conventionally cultured. Interestingly, cells successfully grew when they were subsequently cultured on a dish. Moreover, cells cultured on a plate showed the active form of the YAP protein and the formation of stress fibers and focal adhesions. The results of chemically inhibiting the Rho pathway strongly suggest that this is one of the mechanisms by which the hydrogel promotes type II phenotype maintenance. These results regarding protein expression strongly suggest that the chemical and biophysical properties of the hydrogel have a considerable impact on the transition from ATII to ATI phenotypes. In conclusion, culturing primary alveolar epithelial cells on lung ECM-derived hydrogels may facilitate the prolonged culturing of these cells, and thus help in the research on lung diseases.
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spelling pubmed-91001652022-05-14 Lung Extracellular Matrix Hydrogels Enhance Preservation of Type II Phenotype in Primary Alveolar Epithelial Cells Marhuenda, Esther Villarino, Alvaro Narciso, Maria Leonor Camprubí-Rimblas, Marta Farré, Ramon Gavara, Núria Artigas, Antonio Almendros, Isaac Otero, Jorge Int J Mol Sci Communication One of the main limitations of in vitro studies on lung diseases is the difficulty of maintaining the type II phenotype of alveolar epithelial cells in culture. This fact has previously been related to the translocation of the mechanosensing Yes-associated protein (YAP) to the nuclei and Rho signaling pathway. In this work, we aimed to culture and subculture primary alveolar type II cells on extracellular matrix lung-derived hydrogels to assess their suitability for phenotype maintenance. Cells cultured on lung hydrogels formed monolayers and maintained type II phenotype for a longer time as compared with those conventionally cultured. Interestingly, cells successfully grew when they were subsequently cultured on a dish. Moreover, cells cultured on a plate showed the active form of the YAP protein and the formation of stress fibers and focal adhesions. The results of chemically inhibiting the Rho pathway strongly suggest that this is one of the mechanisms by which the hydrogel promotes type II phenotype maintenance. These results regarding protein expression strongly suggest that the chemical and biophysical properties of the hydrogel have a considerable impact on the transition from ATII to ATI phenotypes. In conclusion, culturing primary alveolar epithelial cells on lung ECM-derived hydrogels may facilitate the prolonged culturing of these cells, and thus help in the research on lung diseases. MDPI 2022-04-28 /pmc/articles/PMC9100165/ /pubmed/35563279 http://dx.doi.org/10.3390/ijms23094888 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Marhuenda, Esther
Villarino, Alvaro
Narciso, Maria Leonor
Camprubí-Rimblas, Marta
Farré, Ramon
Gavara, Núria
Artigas, Antonio
Almendros, Isaac
Otero, Jorge
Lung Extracellular Matrix Hydrogels Enhance Preservation of Type II Phenotype in Primary Alveolar Epithelial Cells
title Lung Extracellular Matrix Hydrogels Enhance Preservation of Type II Phenotype in Primary Alveolar Epithelial Cells
title_full Lung Extracellular Matrix Hydrogels Enhance Preservation of Type II Phenotype in Primary Alveolar Epithelial Cells
title_fullStr Lung Extracellular Matrix Hydrogels Enhance Preservation of Type II Phenotype in Primary Alveolar Epithelial Cells
title_full_unstemmed Lung Extracellular Matrix Hydrogels Enhance Preservation of Type II Phenotype in Primary Alveolar Epithelial Cells
title_short Lung Extracellular Matrix Hydrogels Enhance Preservation of Type II Phenotype in Primary Alveolar Epithelial Cells
title_sort lung extracellular matrix hydrogels enhance preservation of type ii phenotype in primary alveolar epithelial cells
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100165/
https://www.ncbi.nlm.nih.gov/pubmed/35563279
http://dx.doi.org/10.3390/ijms23094888
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