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Close Related Drug-Resistance Beijing Isolates of Mycobacterium tuberculosis Reveal a Different Transcriptomic Signature in a Murine Disease Progression Model
Mycobacterium tuberculosis (MTB) lineage 2/Beijing is associated with high virulence and drug resistance worldwide. In Colombia, the Beijing genotype has circulated since 1997, predominantly on the pacific coast, with the Beijing-Like SIT-190 being more prevalent. This genotype conforms to a drug-re...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100210/ https://www.ncbi.nlm.nih.gov/pubmed/35563545 http://dx.doi.org/10.3390/ijms23095157 |
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author | Cerezo-Cortés, María Irene Rodríguez-Castillo, Juan Germán Mata-Espinosa, Dulce Adriana Bini, Estela Isabel Barrios-Payan, Jorge Zatarain-Barrón, Zyanya Lucia Anzola, Juan Manuel Cornejo-Granados, Fernanda Ochoa-Leyva, Adrian Del Portillo, Patricia Murcia, Martha Isabel Hernández-Pando, Rogelio |
author_facet | Cerezo-Cortés, María Irene Rodríguez-Castillo, Juan Germán Mata-Espinosa, Dulce Adriana Bini, Estela Isabel Barrios-Payan, Jorge Zatarain-Barrón, Zyanya Lucia Anzola, Juan Manuel Cornejo-Granados, Fernanda Ochoa-Leyva, Adrian Del Portillo, Patricia Murcia, Martha Isabel Hernández-Pando, Rogelio |
author_sort | Cerezo-Cortés, María Irene |
collection | PubMed |
description | Mycobacterium tuberculosis (MTB) lineage 2/Beijing is associated with high virulence and drug resistance worldwide. In Colombia, the Beijing genotype has circulated since 1997, predominantly on the pacific coast, with the Beijing-Like SIT-190 being more prevalent. This genotype conforms to a drug-resistant cluster and shows a fatal outcome in patients. To better understand virulence determinants, we performed a transcriptomic analysis with a Beijing-Like SIT-190 isolate (BL-323), and Beijing-Classic SIT-1 isolate (BC-391) in progressive tuberculosis (TB) murine model. Bacterial RNA was extracted from mice lungs on days 3, 14, 28, and 60. On average, 0.6% of the total reads mapped against MTB genomes and of those, 90% against coding genes. The strains were independently associated as determined by hierarchical cluster and multidimensional scaling analysis. Gene ontology showed that in strain BL-323 enriched functions were related to host immune response and hypoxia, while proteolysis and protein folding were enriched in the BC-391 strain. Altogether, our results suggested a differential bacterial transcriptional program when evaluating these two closely related strains. The data presented here could potentially impact the control of this emerging, highly virulent, and drug-resistant genotype. |
format | Online Article Text |
id | pubmed-9100210 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91002102022-05-14 Close Related Drug-Resistance Beijing Isolates of Mycobacterium tuberculosis Reveal a Different Transcriptomic Signature in a Murine Disease Progression Model Cerezo-Cortés, María Irene Rodríguez-Castillo, Juan Germán Mata-Espinosa, Dulce Adriana Bini, Estela Isabel Barrios-Payan, Jorge Zatarain-Barrón, Zyanya Lucia Anzola, Juan Manuel Cornejo-Granados, Fernanda Ochoa-Leyva, Adrian Del Portillo, Patricia Murcia, Martha Isabel Hernández-Pando, Rogelio Int J Mol Sci Article Mycobacterium tuberculosis (MTB) lineage 2/Beijing is associated with high virulence and drug resistance worldwide. In Colombia, the Beijing genotype has circulated since 1997, predominantly on the pacific coast, with the Beijing-Like SIT-190 being more prevalent. This genotype conforms to a drug-resistant cluster and shows a fatal outcome in patients. To better understand virulence determinants, we performed a transcriptomic analysis with a Beijing-Like SIT-190 isolate (BL-323), and Beijing-Classic SIT-1 isolate (BC-391) in progressive tuberculosis (TB) murine model. Bacterial RNA was extracted from mice lungs on days 3, 14, 28, and 60. On average, 0.6% of the total reads mapped against MTB genomes and of those, 90% against coding genes. The strains were independently associated as determined by hierarchical cluster and multidimensional scaling analysis. Gene ontology showed that in strain BL-323 enriched functions were related to host immune response and hypoxia, while proteolysis and protein folding were enriched in the BC-391 strain. Altogether, our results suggested a differential bacterial transcriptional program when evaluating these two closely related strains. The data presented here could potentially impact the control of this emerging, highly virulent, and drug-resistant genotype. MDPI 2022-05-05 /pmc/articles/PMC9100210/ /pubmed/35563545 http://dx.doi.org/10.3390/ijms23095157 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cerezo-Cortés, María Irene Rodríguez-Castillo, Juan Germán Mata-Espinosa, Dulce Adriana Bini, Estela Isabel Barrios-Payan, Jorge Zatarain-Barrón, Zyanya Lucia Anzola, Juan Manuel Cornejo-Granados, Fernanda Ochoa-Leyva, Adrian Del Portillo, Patricia Murcia, Martha Isabel Hernández-Pando, Rogelio Close Related Drug-Resistance Beijing Isolates of Mycobacterium tuberculosis Reveal a Different Transcriptomic Signature in a Murine Disease Progression Model |
title | Close Related Drug-Resistance Beijing Isolates of Mycobacterium tuberculosis Reveal a Different Transcriptomic Signature in a Murine Disease Progression Model |
title_full | Close Related Drug-Resistance Beijing Isolates of Mycobacterium tuberculosis Reveal a Different Transcriptomic Signature in a Murine Disease Progression Model |
title_fullStr | Close Related Drug-Resistance Beijing Isolates of Mycobacterium tuberculosis Reveal a Different Transcriptomic Signature in a Murine Disease Progression Model |
title_full_unstemmed | Close Related Drug-Resistance Beijing Isolates of Mycobacterium tuberculosis Reveal a Different Transcriptomic Signature in a Murine Disease Progression Model |
title_short | Close Related Drug-Resistance Beijing Isolates of Mycobacterium tuberculosis Reveal a Different Transcriptomic Signature in a Murine Disease Progression Model |
title_sort | close related drug-resistance beijing isolates of mycobacterium tuberculosis reveal a different transcriptomic signature in a murine disease progression model |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100210/ https://www.ncbi.nlm.nih.gov/pubmed/35563545 http://dx.doi.org/10.3390/ijms23095157 |
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