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Close Related Drug-Resistance Beijing Isolates of Mycobacterium tuberculosis Reveal a Different Transcriptomic Signature in a Murine Disease Progression Model

Mycobacterium tuberculosis (MTB) lineage 2/Beijing is associated with high virulence and drug resistance worldwide. In Colombia, the Beijing genotype has circulated since 1997, predominantly on the pacific coast, with the Beijing-Like SIT-190 being more prevalent. This genotype conforms to a drug-re...

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Autores principales: Cerezo-Cortés, María Irene, Rodríguez-Castillo, Juan Germán, Mata-Espinosa, Dulce Adriana, Bini, Estela Isabel, Barrios-Payan, Jorge, Zatarain-Barrón, Zyanya Lucia, Anzola, Juan Manuel, Cornejo-Granados, Fernanda, Ochoa-Leyva, Adrian, Del Portillo, Patricia, Murcia, Martha Isabel, Hernández-Pando, Rogelio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100210/
https://www.ncbi.nlm.nih.gov/pubmed/35563545
http://dx.doi.org/10.3390/ijms23095157
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author Cerezo-Cortés, María Irene
Rodríguez-Castillo, Juan Germán
Mata-Espinosa, Dulce Adriana
Bini, Estela Isabel
Barrios-Payan, Jorge
Zatarain-Barrón, Zyanya Lucia
Anzola, Juan Manuel
Cornejo-Granados, Fernanda
Ochoa-Leyva, Adrian
Del Portillo, Patricia
Murcia, Martha Isabel
Hernández-Pando, Rogelio
author_facet Cerezo-Cortés, María Irene
Rodríguez-Castillo, Juan Germán
Mata-Espinosa, Dulce Adriana
Bini, Estela Isabel
Barrios-Payan, Jorge
Zatarain-Barrón, Zyanya Lucia
Anzola, Juan Manuel
Cornejo-Granados, Fernanda
Ochoa-Leyva, Adrian
Del Portillo, Patricia
Murcia, Martha Isabel
Hernández-Pando, Rogelio
author_sort Cerezo-Cortés, María Irene
collection PubMed
description Mycobacterium tuberculosis (MTB) lineage 2/Beijing is associated with high virulence and drug resistance worldwide. In Colombia, the Beijing genotype has circulated since 1997, predominantly on the pacific coast, with the Beijing-Like SIT-190 being more prevalent. This genotype conforms to a drug-resistant cluster and shows a fatal outcome in patients. To better understand virulence determinants, we performed a transcriptomic analysis with a Beijing-Like SIT-190 isolate (BL-323), and Beijing-Classic SIT-1 isolate (BC-391) in progressive tuberculosis (TB) murine model. Bacterial RNA was extracted from mice lungs on days 3, 14, 28, and 60. On average, 0.6% of the total reads mapped against MTB genomes and of those, 90% against coding genes. The strains were independently associated as determined by hierarchical cluster and multidimensional scaling analysis. Gene ontology showed that in strain BL-323 enriched functions were related to host immune response and hypoxia, while proteolysis and protein folding were enriched in the BC-391 strain. Altogether, our results suggested a differential bacterial transcriptional program when evaluating these two closely related strains. The data presented here could potentially impact the control of this emerging, highly virulent, and drug-resistant genotype.
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spelling pubmed-91002102022-05-14 Close Related Drug-Resistance Beijing Isolates of Mycobacterium tuberculosis Reveal a Different Transcriptomic Signature in a Murine Disease Progression Model Cerezo-Cortés, María Irene Rodríguez-Castillo, Juan Germán Mata-Espinosa, Dulce Adriana Bini, Estela Isabel Barrios-Payan, Jorge Zatarain-Barrón, Zyanya Lucia Anzola, Juan Manuel Cornejo-Granados, Fernanda Ochoa-Leyva, Adrian Del Portillo, Patricia Murcia, Martha Isabel Hernández-Pando, Rogelio Int J Mol Sci Article Mycobacterium tuberculosis (MTB) lineage 2/Beijing is associated with high virulence and drug resistance worldwide. In Colombia, the Beijing genotype has circulated since 1997, predominantly on the pacific coast, with the Beijing-Like SIT-190 being more prevalent. This genotype conforms to a drug-resistant cluster and shows a fatal outcome in patients. To better understand virulence determinants, we performed a transcriptomic analysis with a Beijing-Like SIT-190 isolate (BL-323), and Beijing-Classic SIT-1 isolate (BC-391) in progressive tuberculosis (TB) murine model. Bacterial RNA was extracted from mice lungs on days 3, 14, 28, and 60. On average, 0.6% of the total reads mapped against MTB genomes and of those, 90% against coding genes. The strains were independently associated as determined by hierarchical cluster and multidimensional scaling analysis. Gene ontology showed that in strain BL-323 enriched functions were related to host immune response and hypoxia, while proteolysis and protein folding were enriched in the BC-391 strain. Altogether, our results suggested a differential bacterial transcriptional program when evaluating these two closely related strains. The data presented here could potentially impact the control of this emerging, highly virulent, and drug-resistant genotype. MDPI 2022-05-05 /pmc/articles/PMC9100210/ /pubmed/35563545 http://dx.doi.org/10.3390/ijms23095157 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cerezo-Cortés, María Irene
Rodríguez-Castillo, Juan Germán
Mata-Espinosa, Dulce Adriana
Bini, Estela Isabel
Barrios-Payan, Jorge
Zatarain-Barrón, Zyanya Lucia
Anzola, Juan Manuel
Cornejo-Granados, Fernanda
Ochoa-Leyva, Adrian
Del Portillo, Patricia
Murcia, Martha Isabel
Hernández-Pando, Rogelio
Close Related Drug-Resistance Beijing Isolates of Mycobacterium tuberculosis Reveal a Different Transcriptomic Signature in a Murine Disease Progression Model
title Close Related Drug-Resistance Beijing Isolates of Mycobacterium tuberculosis Reveal a Different Transcriptomic Signature in a Murine Disease Progression Model
title_full Close Related Drug-Resistance Beijing Isolates of Mycobacterium tuberculosis Reveal a Different Transcriptomic Signature in a Murine Disease Progression Model
title_fullStr Close Related Drug-Resistance Beijing Isolates of Mycobacterium tuberculosis Reveal a Different Transcriptomic Signature in a Murine Disease Progression Model
title_full_unstemmed Close Related Drug-Resistance Beijing Isolates of Mycobacterium tuberculosis Reveal a Different Transcriptomic Signature in a Murine Disease Progression Model
title_short Close Related Drug-Resistance Beijing Isolates of Mycobacterium tuberculosis Reveal a Different Transcriptomic Signature in a Murine Disease Progression Model
title_sort close related drug-resistance beijing isolates of mycobacterium tuberculosis reveal a different transcriptomic signature in a murine disease progression model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100210/
https://www.ncbi.nlm.nih.gov/pubmed/35563545
http://dx.doi.org/10.3390/ijms23095157
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