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Gardnerella vaginalis in Recurrent Urinary Tract Infection Is Associated with Dysbiosis of the Bladder Microbiome

Recent studies on the urine microbiome have highlighted the importance of the gut–vagina–bladder axis in recurrent urinary tract infection (rUTI). In particular, the role of Gardnerella as a covert pathogen that activates E. coli in animal experiments has been reported. Herein, we conducted a human...

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Autores principales: Yoo, Jeong-Ju, Song, Ju Sun, Kim, Woong Bin, Yun, Jina, Shin, Hee Bong, Jang, Mi-Ae, Ryu, Chang Beom, Kim, Sung Shin, Chung, Jun Chul, Kuk, Jung Cheol, Shin, Eung Jin, Song, Ho-Yeon, Yu, Byung Chul, Lee, Eek-Sung, Ryu, Seongho, Kim, Jae Heon, Jung, Sung Soo, Kim, Young Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100223/
https://www.ncbi.nlm.nih.gov/pubmed/35566419
http://dx.doi.org/10.3390/jcm11092295
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author Yoo, Jeong-Ju
Song, Ju Sun
Kim, Woong Bin
Yun, Jina
Shin, Hee Bong
Jang, Mi-Ae
Ryu, Chang Beom
Kim, Sung Shin
Chung, Jun Chul
Kuk, Jung Cheol
Shin, Eung Jin
Song, Ho-Yeon
Yu, Byung Chul
Lee, Eek-Sung
Ryu, Seongho
Kim, Jae Heon
Jung, Sung Soo
Kim, Young Ho
author_facet Yoo, Jeong-Ju
Song, Ju Sun
Kim, Woong Bin
Yun, Jina
Shin, Hee Bong
Jang, Mi-Ae
Ryu, Chang Beom
Kim, Sung Shin
Chung, Jun Chul
Kuk, Jung Cheol
Shin, Eung Jin
Song, Ho-Yeon
Yu, Byung Chul
Lee, Eek-Sung
Ryu, Seongho
Kim, Jae Heon
Jung, Sung Soo
Kim, Young Ho
author_sort Yoo, Jeong-Ju
collection PubMed
description Recent studies on the urine microbiome have highlighted the importance of the gut–vagina–bladder axis in recurrent urinary tract infection (rUTI). In particular, the role of Gardnerella as a covert pathogen that activates E. coli in animal experiments has been reported. Herein, we conducted a human bladder microbiome study to investigate the effect of Gardnerella on rUTI. Urine 16S ribosomal RNA gene sequencing via transurethral catheterization was conducted in the normal control group (NC) (n = 18) and rUTI group (n = 78). The positive detection rate of Gardnerella species did not differ between the NC and rUTI groups (22.2% vs. 18.0%, p = 0.677). In addition, the Gardnerella-positive NC and Gardnerella-positive rUTI groups showed similar levels of microbiome diversity. The Gardnerella-positive group was categorized into three subgroups: the Escherichia-dominant group, Gardnerella-dominant group, and Lactobacillus-dominant group. All of the Escherichia-dominant groups were associated with rUTI. The Gardnerella-dominant or Lactobacillus-dominant groups expressed rUTI with symptoms when risk factors such as the degree of Gardnerella proliferation or causative agents of bacterial vaginosis were present. The presence of Gardnerella in the urine is considered to be related to rUTI depending on other risk factors. New guideline recommendations regarding antibiotic selection based on a novel method to detect the cause of rUTI may be required to reduce antibiotic resistance.
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spelling pubmed-91002232022-05-14 Gardnerella vaginalis in Recurrent Urinary Tract Infection Is Associated with Dysbiosis of the Bladder Microbiome Yoo, Jeong-Ju Song, Ju Sun Kim, Woong Bin Yun, Jina Shin, Hee Bong Jang, Mi-Ae Ryu, Chang Beom Kim, Sung Shin Chung, Jun Chul Kuk, Jung Cheol Shin, Eung Jin Song, Ho-Yeon Yu, Byung Chul Lee, Eek-Sung Ryu, Seongho Kim, Jae Heon Jung, Sung Soo Kim, Young Ho J Clin Med Article Recent studies on the urine microbiome have highlighted the importance of the gut–vagina–bladder axis in recurrent urinary tract infection (rUTI). In particular, the role of Gardnerella as a covert pathogen that activates E. coli in animal experiments has been reported. Herein, we conducted a human bladder microbiome study to investigate the effect of Gardnerella on rUTI. Urine 16S ribosomal RNA gene sequencing via transurethral catheterization was conducted in the normal control group (NC) (n = 18) and rUTI group (n = 78). The positive detection rate of Gardnerella species did not differ between the NC and rUTI groups (22.2% vs. 18.0%, p = 0.677). In addition, the Gardnerella-positive NC and Gardnerella-positive rUTI groups showed similar levels of microbiome diversity. The Gardnerella-positive group was categorized into three subgroups: the Escherichia-dominant group, Gardnerella-dominant group, and Lactobacillus-dominant group. All of the Escherichia-dominant groups were associated with rUTI. The Gardnerella-dominant or Lactobacillus-dominant groups expressed rUTI with symptoms when risk factors such as the degree of Gardnerella proliferation or causative agents of bacterial vaginosis were present. The presence of Gardnerella in the urine is considered to be related to rUTI depending on other risk factors. New guideline recommendations regarding antibiotic selection based on a novel method to detect the cause of rUTI may be required to reduce antibiotic resistance. MDPI 2022-04-20 /pmc/articles/PMC9100223/ /pubmed/35566419 http://dx.doi.org/10.3390/jcm11092295 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yoo, Jeong-Ju
Song, Ju Sun
Kim, Woong Bin
Yun, Jina
Shin, Hee Bong
Jang, Mi-Ae
Ryu, Chang Beom
Kim, Sung Shin
Chung, Jun Chul
Kuk, Jung Cheol
Shin, Eung Jin
Song, Ho-Yeon
Yu, Byung Chul
Lee, Eek-Sung
Ryu, Seongho
Kim, Jae Heon
Jung, Sung Soo
Kim, Young Ho
Gardnerella vaginalis in Recurrent Urinary Tract Infection Is Associated with Dysbiosis of the Bladder Microbiome
title Gardnerella vaginalis in Recurrent Urinary Tract Infection Is Associated with Dysbiosis of the Bladder Microbiome
title_full Gardnerella vaginalis in Recurrent Urinary Tract Infection Is Associated with Dysbiosis of the Bladder Microbiome
title_fullStr Gardnerella vaginalis in Recurrent Urinary Tract Infection Is Associated with Dysbiosis of the Bladder Microbiome
title_full_unstemmed Gardnerella vaginalis in Recurrent Urinary Tract Infection Is Associated with Dysbiosis of the Bladder Microbiome
title_short Gardnerella vaginalis in Recurrent Urinary Tract Infection Is Associated with Dysbiosis of the Bladder Microbiome
title_sort gardnerella vaginalis in recurrent urinary tract infection is associated with dysbiosis of the bladder microbiome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100223/
https://www.ncbi.nlm.nih.gov/pubmed/35566419
http://dx.doi.org/10.3390/jcm11092295
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