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Counteractive Effects of IL-33 and IL-37 on Inflammation in Osteoarthritis †
Osteoarthritis (OA) is a chronic inflammatory disease where pro-inflammatory cytokines, damage-associated molecular patterns (DAMPs), and macrophages play a crucial role. However, the interactive role of these mediators, the exact cause precipitating OA and definitive treatment for OA are not known...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100324/ https://www.ncbi.nlm.nih.gov/pubmed/35565085 http://dx.doi.org/10.3390/ijerph19095690 |
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author | Rai, Vikrant Dilisio, Matthew F. Samadi, Farial Agrawal, Devendra K. |
author_facet | Rai, Vikrant Dilisio, Matthew F. Samadi, Farial Agrawal, Devendra K. |
author_sort | Rai, Vikrant |
collection | PubMed |
description | Osteoarthritis (OA) is a chronic inflammatory disease where pro-inflammatory cytokines, damage-associated molecular patterns (DAMPs), and macrophages play a crucial role. However, the interactive role of these mediators, the exact cause precipitating OA and definitive treatment for OA are not known yet. Moreover, the interactive role of interleukin (IL)-33 and IL-37 with other factors in the pathogenesis of OA has not been discussed elaborately. In this study, we analyzed the expression of IL-33 and IL-37 in human OA knee and hip joint cartilage tissues. The effect of increased DAMPs, IL-33, and IL-37 on IL-6, tumor necrosis factor (TNF)-α, toll-like receptors (TLRs), and matrix metalloproteinases (MMPs) expression was delineated using human normal and osteoarthritic chondrocytes. The effect of anti-inflammatory cytokine IL-37 on various mediators of inflammation in the presence of IL-33, rHMGB-1, and LPS was investigated to delineate the effects of IL-37. Further, the effects of blocking IL-33 downstream signaling and the effects of IL-33 and IL-37 on macrophage polarization were assessed along with examining the macrophage phenotypes in human OA cartilage tissues. The results of this study revealed increased expression of IL-33 in OA cartilage and that IL-33 increases IL-6, TNF-α, TLRs, and MMPs expression and favors phenotypic conversion towards the M1 phenotype, while IL-37 and blocking IL-33 receptor ST2 have opposite effects. Overall, the results suggest that blocking IL-33 and increasing IL-37 act synergistically to attenuate inflammation and might serve as potential therapeutics in OA. |
format | Online Article Text |
id | pubmed-9100324 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91003242022-05-14 Counteractive Effects of IL-33 and IL-37 on Inflammation in Osteoarthritis † Rai, Vikrant Dilisio, Matthew F. Samadi, Farial Agrawal, Devendra K. Int J Environ Res Public Health Article Osteoarthritis (OA) is a chronic inflammatory disease where pro-inflammatory cytokines, damage-associated molecular patterns (DAMPs), and macrophages play a crucial role. However, the interactive role of these mediators, the exact cause precipitating OA and definitive treatment for OA are not known yet. Moreover, the interactive role of interleukin (IL)-33 and IL-37 with other factors in the pathogenesis of OA has not been discussed elaborately. In this study, we analyzed the expression of IL-33 and IL-37 in human OA knee and hip joint cartilage tissues. The effect of increased DAMPs, IL-33, and IL-37 on IL-6, tumor necrosis factor (TNF)-α, toll-like receptors (TLRs), and matrix metalloproteinases (MMPs) expression was delineated using human normal and osteoarthritic chondrocytes. The effect of anti-inflammatory cytokine IL-37 on various mediators of inflammation in the presence of IL-33, rHMGB-1, and LPS was investigated to delineate the effects of IL-37. Further, the effects of blocking IL-33 downstream signaling and the effects of IL-33 and IL-37 on macrophage polarization were assessed along with examining the macrophage phenotypes in human OA cartilage tissues. The results of this study revealed increased expression of IL-33 in OA cartilage and that IL-33 increases IL-6, TNF-α, TLRs, and MMPs expression and favors phenotypic conversion towards the M1 phenotype, while IL-37 and blocking IL-33 receptor ST2 have opposite effects. Overall, the results suggest that blocking IL-33 and increasing IL-37 act synergistically to attenuate inflammation and might serve as potential therapeutics in OA. MDPI 2022-05-07 /pmc/articles/PMC9100324/ /pubmed/35565085 http://dx.doi.org/10.3390/ijerph19095690 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Rai, Vikrant Dilisio, Matthew F. Samadi, Farial Agrawal, Devendra K. Counteractive Effects of IL-33 and IL-37 on Inflammation in Osteoarthritis † |
title | Counteractive Effects of IL-33 and IL-37 on Inflammation in Osteoarthritis † |
title_full | Counteractive Effects of IL-33 and IL-37 on Inflammation in Osteoarthritis † |
title_fullStr | Counteractive Effects of IL-33 and IL-37 on Inflammation in Osteoarthritis † |
title_full_unstemmed | Counteractive Effects of IL-33 and IL-37 on Inflammation in Osteoarthritis † |
title_short | Counteractive Effects of IL-33 and IL-37 on Inflammation in Osteoarthritis † |
title_sort | counteractive effects of il-33 and il-37 on inflammation in osteoarthritis † |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100324/ https://www.ncbi.nlm.nih.gov/pubmed/35565085 http://dx.doi.org/10.3390/ijerph19095690 |
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