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Compartment-Specific Differences in the Activation of Monocyte Subpopulations Are Not Affected by Nitric Oxide and Glucocorticoid Treatment in a Model of Resuscitated Porcine Endotoxemic Shock

Inhaled nitric oxide (iNO) remains one of the treatment modalities in shock, and in addition to its vasoactive properties, iNO exerts immunomodulatory effects. We used a porcine model of endotoxemia with shock resuscitation (control) and additional treatment with iNO and a steroid (treatment group)....

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Autores principales: Skirecki, Tomasz, Adamik, Barbara, Frostell, Claes, Pasławska, Urszula, Zieliński, Stanisław, Glatzel-Plucińska, Natalia, Olbromski, Mateusz, Dzięgiel, Piotr, Gozdzik, Waldemar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100570/
https://www.ncbi.nlm.nih.gov/pubmed/35566768
http://dx.doi.org/10.3390/jcm11092641
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author Skirecki, Tomasz
Adamik, Barbara
Frostell, Claes
Pasławska, Urszula
Zieliński, Stanisław
Glatzel-Plucińska, Natalia
Olbromski, Mateusz
Dzięgiel, Piotr
Gozdzik, Waldemar
author_facet Skirecki, Tomasz
Adamik, Barbara
Frostell, Claes
Pasławska, Urszula
Zieliński, Stanisław
Glatzel-Plucińska, Natalia
Olbromski, Mateusz
Dzięgiel, Piotr
Gozdzik, Waldemar
author_sort Skirecki, Tomasz
collection PubMed
description Inhaled nitric oxide (iNO) remains one of the treatment modalities in shock, and in addition to its vasoactive properties, iNO exerts immunomodulatory effects. We used a porcine model of endotoxemia with shock resuscitation (control) and additional treatment with iNO and a steroid (treatment group). After 20 h, bone marrow (BM), peripheral blood (PB), and bronchoalveolar lavage fluid (BALF) were collected to analyze the immunophenotype and mitochondrial membrane potential (Δφ) in three subsets of monocytes. In both groups, SLA-DR expression decreased twofold on the circulating CD14(+)CD163(+) and CD14(−)CD163(+) monocytes, while it did not change on the CD14(+)CD163(+). Δφ increased only in the CD14(−)CD163(+) subpopulation (0.8 vs. 2.0, p < 0.001). The analysis of compartment-specific alterations showed that nearly 100% of BALF CD14(+)CD163(+) and CD14(−)CD163(+) monocytes expressed SLA-DR, and it was higher compared to PB (32% and 20%, p < 0.0001) and BM (93% and 67%, p < 0.001, respectively) counterparts. BALF CD14(+)CD163(+) had a threefold higher Δφ than PB and BM monocytes, while the Δφ of the other subsets was highest in PB monocytes. We confirmed the compartmentalization of the monocyte response during endotoxemic shock, which highlights the importance of studying tissue-resident cells in addition to their circulating counterparts. The iNO/steroid treatment did not further impair monocyte fitness.
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spelling pubmed-91005702022-05-14 Compartment-Specific Differences in the Activation of Monocyte Subpopulations Are Not Affected by Nitric Oxide and Glucocorticoid Treatment in a Model of Resuscitated Porcine Endotoxemic Shock Skirecki, Tomasz Adamik, Barbara Frostell, Claes Pasławska, Urszula Zieliński, Stanisław Glatzel-Plucińska, Natalia Olbromski, Mateusz Dzięgiel, Piotr Gozdzik, Waldemar J Clin Med Article Inhaled nitric oxide (iNO) remains one of the treatment modalities in shock, and in addition to its vasoactive properties, iNO exerts immunomodulatory effects. We used a porcine model of endotoxemia with shock resuscitation (control) and additional treatment with iNO and a steroid (treatment group). After 20 h, bone marrow (BM), peripheral blood (PB), and bronchoalveolar lavage fluid (BALF) were collected to analyze the immunophenotype and mitochondrial membrane potential (Δφ) in three subsets of monocytes. In both groups, SLA-DR expression decreased twofold on the circulating CD14(+)CD163(+) and CD14(−)CD163(+) monocytes, while it did not change on the CD14(+)CD163(+). Δφ increased only in the CD14(−)CD163(+) subpopulation (0.8 vs. 2.0, p < 0.001). The analysis of compartment-specific alterations showed that nearly 100% of BALF CD14(+)CD163(+) and CD14(−)CD163(+) monocytes expressed SLA-DR, and it was higher compared to PB (32% and 20%, p < 0.0001) and BM (93% and 67%, p < 0.001, respectively) counterparts. BALF CD14(+)CD163(+) had a threefold higher Δφ than PB and BM monocytes, while the Δφ of the other subsets was highest in PB monocytes. We confirmed the compartmentalization of the monocyte response during endotoxemic shock, which highlights the importance of studying tissue-resident cells in addition to their circulating counterparts. The iNO/steroid treatment did not further impair monocyte fitness. MDPI 2022-05-08 /pmc/articles/PMC9100570/ /pubmed/35566768 http://dx.doi.org/10.3390/jcm11092641 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Skirecki, Tomasz
Adamik, Barbara
Frostell, Claes
Pasławska, Urszula
Zieliński, Stanisław
Glatzel-Plucińska, Natalia
Olbromski, Mateusz
Dzięgiel, Piotr
Gozdzik, Waldemar
Compartment-Specific Differences in the Activation of Monocyte Subpopulations Are Not Affected by Nitric Oxide and Glucocorticoid Treatment in a Model of Resuscitated Porcine Endotoxemic Shock
title Compartment-Specific Differences in the Activation of Monocyte Subpopulations Are Not Affected by Nitric Oxide and Glucocorticoid Treatment in a Model of Resuscitated Porcine Endotoxemic Shock
title_full Compartment-Specific Differences in the Activation of Monocyte Subpopulations Are Not Affected by Nitric Oxide and Glucocorticoid Treatment in a Model of Resuscitated Porcine Endotoxemic Shock
title_fullStr Compartment-Specific Differences in the Activation of Monocyte Subpopulations Are Not Affected by Nitric Oxide and Glucocorticoid Treatment in a Model of Resuscitated Porcine Endotoxemic Shock
title_full_unstemmed Compartment-Specific Differences in the Activation of Monocyte Subpopulations Are Not Affected by Nitric Oxide and Glucocorticoid Treatment in a Model of Resuscitated Porcine Endotoxemic Shock
title_short Compartment-Specific Differences in the Activation of Monocyte Subpopulations Are Not Affected by Nitric Oxide and Glucocorticoid Treatment in a Model of Resuscitated Porcine Endotoxemic Shock
title_sort compartment-specific differences in the activation of monocyte subpopulations are not affected by nitric oxide and glucocorticoid treatment in a model of resuscitated porcine endotoxemic shock
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100570/
https://www.ncbi.nlm.nih.gov/pubmed/35566768
http://dx.doi.org/10.3390/jcm11092641
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