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Bovine Coronavirus Infects the Respiratory Tract of Cattle Challenged Intranasally

Bovine Coronavirus (BCoV) is a member of a family of viruses associated with both enteric and respiratory diseases in a wide range of hosts. BCoV has been well-established as a causative agent of diarrhea in cattle, however, its role as a respiratory pathogen is controversial. In this study, fifteen...

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Autores principales: Soules, Katelyn R., Rahe, Michael C., Purtle, Lisa, Moeckly, Craig, Stark, Paul, Samson, Clay, Knittel, Jeffrey P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100586/
https://www.ncbi.nlm.nih.gov/pubmed/35573402
http://dx.doi.org/10.3389/fvets.2022.878240
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author Soules, Katelyn R.
Rahe, Michael C.
Purtle, Lisa
Moeckly, Craig
Stark, Paul
Samson, Clay
Knittel, Jeffrey P.
author_facet Soules, Katelyn R.
Rahe, Michael C.
Purtle, Lisa
Moeckly, Craig
Stark, Paul
Samson, Clay
Knittel, Jeffrey P.
author_sort Soules, Katelyn R.
collection PubMed
description Bovine Coronavirus (BCoV) is a member of a family of viruses associated with both enteric and respiratory diseases in a wide range of hosts. BCoV has been well-established as a causative agent of diarrhea in cattle, however, its role as a respiratory pathogen is controversial. In this study, fifteen calves were challenged intranasally with virulent BCoV in order to observe the clinical manifestation of the BCoV infection for up to 8 days after initial challenge, looking specifically for indication of symptoms, pathology, and presence of viral infection in the respiratory tract, as compared to six unchallenged control calves. Throughout the study, clinical signs of disease were recorded and nasal swabs were collected daily. Additionally, bronchoalveolar lavage (BAL) was performed at 4 days Post-challenge, and blood and tissue samples were collected from calves at 4, 6, or 8 days Post-challenge to be tested for the presence of BCoV and disease pathology. The data collected support that this BCoV challenge resulted in respiratory infections as evidenced by the isolation of BCoV in BAL fluids and positive qPCR, immunohistochemistry (IHC), and histopathologic lesions in the upper and lower respiratory tissues. This study can thus be added to a growing body of data supporting that BCoV is a respiratory pathogen and contributor to respiratory disease in cattle.
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spelling pubmed-91005862022-05-14 Bovine Coronavirus Infects the Respiratory Tract of Cattle Challenged Intranasally Soules, Katelyn R. Rahe, Michael C. Purtle, Lisa Moeckly, Craig Stark, Paul Samson, Clay Knittel, Jeffrey P. Front Vet Sci Veterinary Science Bovine Coronavirus (BCoV) is a member of a family of viruses associated with both enteric and respiratory diseases in a wide range of hosts. BCoV has been well-established as a causative agent of diarrhea in cattle, however, its role as a respiratory pathogen is controversial. In this study, fifteen calves were challenged intranasally with virulent BCoV in order to observe the clinical manifestation of the BCoV infection for up to 8 days after initial challenge, looking specifically for indication of symptoms, pathology, and presence of viral infection in the respiratory tract, as compared to six unchallenged control calves. Throughout the study, clinical signs of disease were recorded and nasal swabs were collected daily. Additionally, bronchoalveolar lavage (BAL) was performed at 4 days Post-challenge, and blood and tissue samples were collected from calves at 4, 6, or 8 days Post-challenge to be tested for the presence of BCoV and disease pathology. The data collected support that this BCoV challenge resulted in respiratory infections as evidenced by the isolation of BCoV in BAL fluids and positive qPCR, immunohistochemistry (IHC), and histopathologic lesions in the upper and lower respiratory tissues. This study can thus be added to a growing body of data supporting that BCoV is a respiratory pathogen and contributor to respiratory disease in cattle. Frontiers Media S.A. 2022-04-29 /pmc/articles/PMC9100586/ /pubmed/35573402 http://dx.doi.org/10.3389/fvets.2022.878240 Text en Copyright © 2022 Soules, Rahe, Purtle, Moeckly, Stark, Samson and Knittel. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Soules, Katelyn R.
Rahe, Michael C.
Purtle, Lisa
Moeckly, Craig
Stark, Paul
Samson, Clay
Knittel, Jeffrey P.
Bovine Coronavirus Infects the Respiratory Tract of Cattle Challenged Intranasally
title Bovine Coronavirus Infects the Respiratory Tract of Cattle Challenged Intranasally
title_full Bovine Coronavirus Infects the Respiratory Tract of Cattle Challenged Intranasally
title_fullStr Bovine Coronavirus Infects the Respiratory Tract of Cattle Challenged Intranasally
title_full_unstemmed Bovine Coronavirus Infects the Respiratory Tract of Cattle Challenged Intranasally
title_short Bovine Coronavirus Infects the Respiratory Tract of Cattle Challenged Intranasally
title_sort bovine coronavirus infects the respiratory tract of cattle challenged intranasally
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100586/
https://www.ncbi.nlm.nih.gov/pubmed/35573402
http://dx.doi.org/10.3389/fvets.2022.878240
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