Cargando…

Molecular Identification and Phylogenetic Analysis of Ascarids in Wild Animals

Ascarid nematodes are the most common and harmful nematodes parasites in animals. By analyzing genetic variation, this study explores the genetic and phylogenetic relationship among ascarids from 11 different hosts. This study collected ascarid samples from the feces of nine animal species in Changs...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Shu-Yu, Qiu, Qi-Guan, Mo, Hai-Long, Gong, Teng-Fang, Li, Fen, He, Jun-Lin, Li, Wen-Chao, Xie, Xin-Rui, Liu, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100682/
https://www.ncbi.nlm.nih.gov/pubmed/35573413
http://dx.doi.org/10.3389/fvets.2022.891672
_version_ 1784706905292144640
author Chen, Shu-Yu
Qiu, Qi-Guan
Mo, Hai-Long
Gong, Teng-Fang
Li, Fen
He, Jun-Lin
Li, Wen-Chao
Xie, Xin-Rui
Liu, Wei
author_facet Chen, Shu-Yu
Qiu, Qi-Guan
Mo, Hai-Long
Gong, Teng-Fang
Li, Fen
He, Jun-Lin
Li, Wen-Chao
Xie, Xin-Rui
Liu, Wei
author_sort Chen, Shu-Yu
collection PubMed
description Ascarid nematodes are the most common and harmful nematodes parasites in animals. By analyzing genetic variation, this study explores the genetic and phylogenetic relationship among ascarids from 11 different hosts. This study collected ascarid samples from the feces of nine animal species in Changsha Ecological Zoo of Hunan Province and two animal kinds in the College of Veterinary Medicine of Hunan Agricultural University. The mitochondrial gene (pcox1) and ribosomal ITS sequences were amplified, sequenced, and analyzed by PCR to identify the species of the samples. The phylogenetic tree was constructed based on two genes (cox1 and ITS) by the Neighbor-joining method, and the phylogenetic relationship was analyzed. The sequencing results showed that the sequence lengths of pcox1 and ITS genes in the samples were 441 bp and 838–1,177 bp, respectively. The difference rates were 0.00–1.70% in pcox1 gene and 0.00–7.30% in ITS gene. Phylogenetic analysis showed that ascarid worms from the white lion, Northeast tiger, South China tiger and cheetah were identified as Toxascaris leonina. Ascarids from the zebra were identified as Parascaris equorum, while those from chicken and peacocks were identified as Ascaridia galli. Ascarids of wolf and dog origin were Toxocara canis, the snake ascarids belonged to Ophidascaris filaria, and the bear ascarids belonged to Baylisascaris transfuga. There was a significant gap between different kinds of ascarid worms. We found that these two mitochondrial genes pcox1 and ITS showed a common characteristic that the intraspecific differences were significantly smaller than the interspecific differences, confirming that these two genes could be used as interspecific genetic markers for molecular identification of different ascarids origins. The intraspecific variation rate of the ITS gene was higher than that of pcox1, indicating that ITS can also be used in the genetic research of Ascaris species development. This study revealed the genetic evolution and phylogeny of ascarids in wild animals, and our results will help prevent and control ascarids in wild animals.
format Online
Article
Text
id pubmed-9100682
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-91006822022-05-14 Molecular Identification and Phylogenetic Analysis of Ascarids in Wild Animals Chen, Shu-Yu Qiu, Qi-Guan Mo, Hai-Long Gong, Teng-Fang Li, Fen He, Jun-Lin Li, Wen-Chao Xie, Xin-Rui Liu, Wei Front Vet Sci Veterinary Science Ascarid nematodes are the most common and harmful nematodes parasites in animals. By analyzing genetic variation, this study explores the genetic and phylogenetic relationship among ascarids from 11 different hosts. This study collected ascarid samples from the feces of nine animal species in Changsha Ecological Zoo of Hunan Province and two animal kinds in the College of Veterinary Medicine of Hunan Agricultural University. The mitochondrial gene (pcox1) and ribosomal ITS sequences were amplified, sequenced, and analyzed by PCR to identify the species of the samples. The phylogenetic tree was constructed based on two genes (cox1 and ITS) by the Neighbor-joining method, and the phylogenetic relationship was analyzed. The sequencing results showed that the sequence lengths of pcox1 and ITS genes in the samples were 441 bp and 838–1,177 bp, respectively. The difference rates were 0.00–1.70% in pcox1 gene and 0.00–7.30% in ITS gene. Phylogenetic analysis showed that ascarid worms from the white lion, Northeast tiger, South China tiger and cheetah were identified as Toxascaris leonina. Ascarids from the zebra were identified as Parascaris equorum, while those from chicken and peacocks were identified as Ascaridia galli. Ascarids of wolf and dog origin were Toxocara canis, the snake ascarids belonged to Ophidascaris filaria, and the bear ascarids belonged to Baylisascaris transfuga. There was a significant gap between different kinds of ascarid worms. We found that these two mitochondrial genes pcox1 and ITS showed a common characteristic that the intraspecific differences were significantly smaller than the interspecific differences, confirming that these two genes could be used as interspecific genetic markers for molecular identification of different ascarids origins. The intraspecific variation rate of the ITS gene was higher than that of pcox1, indicating that ITS can also be used in the genetic research of Ascaris species development. This study revealed the genetic evolution and phylogeny of ascarids in wild animals, and our results will help prevent and control ascarids in wild animals. Frontiers Media S.A. 2022-04-29 /pmc/articles/PMC9100682/ /pubmed/35573413 http://dx.doi.org/10.3389/fvets.2022.891672 Text en Copyright © 2022 Chen, Qiu, Mo, Gong, Li, He, Li, Xie and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Chen, Shu-Yu
Qiu, Qi-Guan
Mo, Hai-Long
Gong, Teng-Fang
Li, Fen
He, Jun-Lin
Li, Wen-Chao
Xie, Xin-Rui
Liu, Wei
Molecular Identification and Phylogenetic Analysis of Ascarids in Wild Animals
title Molecular Identification and Phylogenetic Analysis of Ascarids in Wild Animals
title_full Molecular Identification and Phylogenetic Analysis of Ascarids in Wild Animals
title_fullStr Molecular Identification and Phylogenetic Analysis of Ascarids in Wild Animals
title_full_unstemmed Molecular Identification and Phylogenetic Analysis of Ascarids in Wild Animals
title_short Molecular Identification and Phylogenetic Analysis of Ascarids in Wild Animals
title_sort molecular identification and phylogenetic analysis of ascarids in wild animals
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100682/
https://www.ncbi.nlm.nih.gov/pubmed/35573413
http://dx.doi.org/10.3389/fvets.2022.891672
work_keys_str_mv AT chenshuyu molecularidentificationandphylogeneticanalysisofascaridsinwildanimals
AT qiuqiguan molecularidentificationandphylogeneticanalysisofascaridsinwildanimals
AT mohailong molecularidentificationandphylogeneticanalysisofascaridsinwildanimals
AT gongtengfang molecularidentificationandphylogeneticanalysisofascaridsinwildanimals
AT lifen molecularidentificationandphylogeneticanalysisofascaridsinwildanimals
AT hejunlin molecularidentificationandphylogeneticanalysisofascaridsinwildanimals
AT liwenchao molecularidentificationandphylogeneticanalysisofascaridsinwildanimals
AT xiexinrui molecularidentificationandphylogeneticanalysisofascaridsinwildanimals
AT liuwei molecularidentificationandphylogeneticanalysisofascaridsinwildanimals