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Fungal Microbiota Dysbiosis and Ecological Alterations in Gastric Cancer

Changes in bacteriome composition have a strong association with gastric cancer (GC). However, the relationship between stomach fungal microbiota composition and human host immune factors remains largely unknown. With high-throughput internal transcribed spacer region 2 (ITS2) sequencing, we charact...

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Autores principales: Yang, Ping, Zhang, Xiaoshan, Xu, Rui, Adeel, Khan, Lu, Xiaofeng, Chen, Min, Shen, Han, Li, Zhiyang, Xu, Zhipeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100745/
https://www.ncbi.nlm.nih.gov/pubmed/35572666
http://dx.doi.org/10.3389/fmicb.2022.889694
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author Yang, Ping
Zhang, Xiaoshan
Xu, Rui
Adeel, Khan
Lu, Xiaofeng
Chen, Min
Shen, Han
Li, Zhiyang
Xu, Zhipeng
author_facet Yang, Ping
Zhang, Xiaoshan
Xu, Rui
Adeel, Khan
Lu, Xiaofeng
Chen, Min
Shen, Han
Li, Zhiyang
Xu, Zhipeng
author_sort Yang, Ping
collection PubMed
description Changes in bacteriome composition have a strong association with gastric cancer (GC). However, the relationship between stomach fungal microbiota composition and human host immune factors remains largely unknown. With high-throughput internal transcribed spacer region 2 (ITS2) sequencing, we characterized gastric fungal microbiome among the GC (n = 22), matched para-GC (n = 22), and healthy individuals (n = 11). A total of 4.5 million valid tags were generated and stratified into 1,631 operational taxonomic units (OTUs), and 10 phyla and 301 genera were identified. The presence of GC was associated with a distinct gastric fungal mycobiome signature, characterized by a decreased biodiversity and richness and significant differences in fungal composition. In addition, fungal dysbiosis was reflected by the increased ratio of Basidiomycota to Ascomycota and a higher proportion of opportunistic fungi, such as Cutaneotrichosporon and Malassezia, as well as the loss of Rhizopus and Rhodotorula during the progression of cancers. A panel of GC-associated fungi (e.g., Cutaneotrichosporon and Rhodotorula) was found to adequately exhibit diagnostic value. Furthermore, the mRNA levels of cytokines and chemokines were detected and correlated with the specific fungal dysbiosis, indicating the possible mechanism of GC. This study reveals GC-associated mycobiome dysbiosis characterized by altered fungal composition and ecology and suggests that the fungal mycobiome might play a role in the pathogenesis of GC.
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spelling pubmed-91007452022-05-14 Fungal Microbiota Dysbiosis and Ecological Alterations in Gastric Cancer Yang, Ping Zhang, Xiaoshan Xu, Rui Adeel, Khan Lu, Xiaofeng Chen, Min Shen, Han Li, Zhiyang Xu, Zhipeng Front Microbiol Microbiology Changes in bacteriome composition have a strong association with gastric cancer (GC). However, the relationship between stomach fungal microbiota composition and human host immune factors remains largely unknown. With high-throughput internal transcribed spacer region 2 (ITS2) sequencing, we characterized gastric fungal microbiome among the GC (n = 22), matched para-GC (n = 22), and healthy individuals (n = 11). A total of 4.5 million valid tags were generated and stratified into 1,631 operational taxonomic units (OTUs), and 10 phyla and 301 genera were identified. The presence of GC was associated with a distinct gastric fungal mycobiome signature, characterized by a decreased biodiversity and richness and significant differences in fungal composition. In addition, fungal dysbiosis was reflected by the increased ratio of Basidiomycota to Ascomycota and a higher proportion of opportunistic fungi, such as Cutaneotrichosporon and Malassezia, as well as the loss of Rhizopus and Rhodotorula during the progression of cancers. A panel of GC-associated fungi (e.g., Cutaneotrichosporon and Rhodotorula) was found to adequately exhibit diagnostic value. Furthermore, the mRNA levels of cytokines and chemokines were detected and correlated with the specific fungal dysbiosis, indicating the possible mechanism of GC. This study reveals GC-associated mycobiome dysbiosis characterized by altered fungal composition and ecology and suggests that the fungal mycobiome might play a role in the pathogenesis of GC. Frontiers Media S.A. 2022-04-29 /pmc/articles/PMC9100745/ /pubmed/35572666 http://dx.doi.org/10.3389/fmicb.2022.889694 Text en Copyright © 2022 Yang, Zhang, Xu, Adeel, Lu, Chen, Shen, Li and Xu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Yang, Ping
Zhang, Xiaoshan
Xu, Rui
Adeel, Khan
Lu, Xiaofeng
Chen, Min
Shen, Han
Li, Zhiyang
Xu, Zhipeng
Fungal Microbiota Dysbiosis and Ecological Alterations in Gastric Cancer
title Fungal Microbiota Dysbiosis and Ecological Alterations in Gastric Cancer
title_full Fungal Microbiota Dysbiosis and Ecological Alterations in Gastric Cancer
title_fullStr Fungal Microbiota Dysbiosis and Ecological Alterations in Gastric Cancer
title_full_unstemmed Fungal Microbiota Dysbiosis and Ecological Alterations in Gastric Cancer
title_short Fungal Microbiota Dysbiosis and Ecological Alterations in Gastric Cancer
title_sort fungal microbiota dysbiosis and ecological alterations in gastric cancer
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100745/
https://www.ncbi.nlm.nih.gov/pubmed/35572666
http://dx.doi.org/10.3389/fmicb.2022.889694
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