Cargando…

Overexpression of EgrIAA20 from Eucalyptus grandis, a Non-Canonical Aux/IAA Gene, Specifically Decouples Lignification of the Different Cell-Types in Arabidopsis Secondary Xylem

Wood (secondary xylem) formation is regulated by auxin, which plays a pivotal role as an integrator of developmental and environmental cues. However, our current knowledge of auxin-signaling during wood formation is incomplete. Our previous genome-wide analysis of Aux/IAAs in Eucalyptus grandis show...

Descripción completa

Detalles Bibliográficos
Autores principales: Yu, Hong, Liu, Mingjun, Zhu, Zhangsheng, Wu, Aiming, Mounet, Fabien, Pesquet, Edouard, Grima-Pettenati, Jacqueline, Cassan-Wang, Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100763/
https://www.ncbi.nlm.nih.gov/pubmed/35563457
http://dx.doi.org/10.3390/ijms23095068
_version_ 1784706926428291072
author Yu, Hong
Liu, Mingjun
Zhu, Zhangsheng
Wu, Aiming
Mounet, Fabien
Pesquet, Edouard
Grima-Pettenati, Jacqueline
Cassan-Wang, Hua
author_facet Yu, Hong
Liu, Mingjun
Zhu, Zhangsheng
Wu, Aiming
Mounet, Fabien
Pesquet, Edouard
Grima-Pettenati, Jacqueline
Cassan-Wang, Hua
author_sort Yu, Hong
collection PubMed
description Wood (secondary xylem) formation is regulated by auxin, which plays a pivotal role as an integrator of developmental and environmental cues. However, our current knowledge of auxin-signaling during wood formation is incomplete. Our previous genome-wide analysis of Aux/IAAs in Eucalyptus grandis showed the presence of the non-canonical paralog member EgrIAA20 that is preferentially expressed in cambium. We analyzed its cellular localization using a GFP fusion protein and its transcriptional activity using transactivation assays, and demonstrated its nuclear localization and strong auxin response repressor activity. In addition, we functionally tested the role of EgrIAA20 by constitutive overexpression in Arabidopsis to investigate for phenotypic changes in secondary xylem formation. Transgenic Arabidopsis plants overexpressing EgrIAA20 were smaller and displayed impaired development of secondary fibers, but not of other wood cell types. The inhibition in fiber development specifically affected their cell wall lignification. We performed yeast-two-hybrid assays to identify EgrIAA20 protein partners during wood formation in Eucalyptus, and identified EgrIAA9A, whose ortholog PtoIAA9 in poplar is also known to be involved in wood formation. Altogether, we showed that EgrIAA20 is an important auxin signaling component specifically involved in controlling the lignification of wood fibers.
format Online
Article
Text
id pubmed-9100763
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-91007632022-05-14 Overexpression of EgrIAA20 from Eucalyptus grandis, a Non-Canonical Aux/IAA Gene, Specifically Decouples Lignification of the Different Cell-Types in Arabidopsis Secondary Xylem Yu, Hong Liu, Mingjun Zhu, Zhangsheng Wu, Aiming Mounet, Fabien Pesquet, Edouard Grima-Pettenati, Jacqueline Cassan-Wang, Hua Int J Mol Sci Article Wood (secondary xylem) formation is regulated by auxin, which plays a pivotal role as an integrator of developmental and environmental cues. However, our current knowledge of auxin-signaling during wood formation is incomplete. Our previous genome-wide analysis of Aux/IAAs in Eucalyptus grandis showed the presence of the non-canonical paralog member EgrIAA20 that is preferentially expressed in cambium. We analyzed its cellular localization using a GFP fusion protein and its transcriptional activity using transactivation assays, and demonstrated its nuclear localization and strong auxin response repressor activity. In addition, we functionally tested the role of EgrIAA20 by constitutive overexpression in Arabidopsis to investigate for phenotypic changes in secondary xylem formation. Transgenic Arabidopsis plants overexpressing EgrIAA20 were smaller and displayed impaired development of secondary fibers, but not of other wood cell types. The inhibition in fiber development specifically affected their cell wall lignification. We performed yeast-two-hybrid assays to identify EgrIAA20 protein partners during wood formation in Eucalyptus, and identified EgrIAA9A, whose ortholog PtoIAA9 in poplar is also known to be involved in wood formation. Altogether, we showed that EgrIAA20 is an important auxin signaling component specifically involved in controlling the lignification of wood fibers. MDPI 2022-05-03 /pmc/articles/PMC9100763/ /pubmed/35563457 http://dx.doi.org/10.3390/ijms23095068 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yu, Hong
Liu, Mingjun
Zhu, Zhangsheng
Wu, Aiming
Mounet, Fabien
Pesquet, Edouard
Grima-Pettenati, Jacqueline
Cassan-Wang, Hua
Overexpression of EgrIAA20 from Eucalyptus grandis, a Non-Canonical Aux/IAA Gene, Specifically Decouples Lignification of the Different Cell-Types in Arabidopsis Secondary Xylem
title Overexpression of EgrIAA20 from Eucalyptus grandis, a Non-Canonical Aux/IAA Gene, Specifically Decouples Lignification of the Different Cell-Types in Arabidopsis Secondary Xylem
title_full Overexpression of EgrIAA20 from Eucalyptus grandis, a Non-Canonical Aux/IAA Gene, Specifically Decouples Lignification of the Different Cell-Types in Arabidopsis Secondary Xylem
title_fullStr Overexpression of EgrIAA20 from Eucalyptus grandis, a Non-Canonical Aux/IAA Gene, Specifically Decouples Lignification of the Different Cell-Types in Arabidopsis Secondary Xylem
title_full_unstemmed Overexpression of EgrIAA20 from Eucalyptus grandis, a Non-Canonical Aux/IAA Gene, Specifically Decouples Lignification of the Different Cell-Types in Arabidopsis Secondary Xylem
title_short Overexpression of EgrIAA20 from Eucalyptus grandis, a Non-Canonical Aux/IAA Gene, Specifically Decouples Lignification of the Different Cell-Types in Arabidopsis Secondary Xylem
title_sort overexpression of egriaa20 from eucalyptus grandis, a non-canonical aux/iaa gene, specifically decouples lignification of the different cell-types in arabidopsis secondary xylem
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100763/
https://www.ncbi.nlm.nih.gov/pubmed/35563457
http://dx.doi.org/10.3390/ijms23095068
work_keys_str_mv AT yuhong overexpressionofegriaa20fromeucalyptusgrandisanoncanonicalauxiaagenespecificallydecoupleslignificationofthedifferentcelltypesinarabidopsissecondaryxylem
AT liumingjun overexpressionofegriaa20fromeucalyptusgrandisanoncanonicalauxiaagenespecificallydecoupleslignificationofthedifferentcelltypesinarabidopsissecondaryxylem
AT zhuzhangsheng overexpressionofegriaa20fromeucalyptusgrandisanoncanonicalauxiaagenespecificallydecoupleslignificationofthedifferentcelltypesinarabidopsissecondaryxylem
AT wuaiming overexpressionofegriaa20fromeucalyptusgrandisanoncanonicalauxiaagenespecificallydecoupleslignificationofthedifferentcelltypesinarabidopsissecondaryxylem
AT mounetfabien overexpressionofegriaa20fromeucalyptusgrandisanoncanonicalauxiaagenespecificallydecoupleslignificationofthedifferentcelltypesinarabidopsissecondaryxylem
AT pesquetedouard overexpressionofegriaa20fromeucalyptusgrandisanoncanonicalauxiaagenespecificallydecoupleslignificationofthedifferentcelltypesinarabidopsissecondaryxylem
AT grimapettenatijacqueline overexpressionofegriaa20fromeucalyptusgrandisanoncanonicalauxiaagenespecificallydecoupleslignificationofthedifferentcelltypesinarabidopsissecondaryxylem
AT cassanwanghua overexpressionofegriaa20fromeucalyptusgrandisanoncanonicalauxiaagenespecificallydecoupleslignificationofthedifferentcelltypesinarabidopsissecondaryxylem