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Source-Sink Dynamics in Field-Grown Durum Wheat Under Contrasting Nitrogen Supplies: Key Role of Non-Foliar Organs During Grain Filling

The integration of high-throughput phenotyping and metabolic approaches is a suitable strategy to study the genotype-by-environment interaction and identify novel traits for crop improvement from canopy to an organ level. Our aims were to study the phenotypic and metabolic traits that are related to...

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Autores principales: Martínez-Peña, Raquel, Schlereth, Armin, Höhne, Melanie, Encke, Beatrice, Morcuende, Rosa, Nieto-Taladriz, María Teresa, Araus, José Luis, Aparicio, Nieves, Vicente, Rubén
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100808/
https://www.ncbi.nlm.nih.gov/pubmed/35574116
http://dx.doi.org/10.3389/fpls.2022.869680
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author Martínez-Peña, Raquel
Schlereth, Armin
Höhne, Melanie
Encke, Beatrice
Morcuende, Rosa
Nieto-Taladriz, María Teresa
Araus, José Luis
Aparicio, Nieves
Vicente, Rubén
author_facet Martínez-Peña, Raquel
Schlereth, Armin
Höhne, Melanie
Encke, Beatrice
Morcuende, Rosa
Nieto-Taladriz, María Teresa
Araus, José Luis
Aparicio, Nieves
Vicente, Rubén
author_sort Martínez-Peña, Raquel
collection PubMed
description The integration of high-throughput phenotyping and metabolic approaches is a suitable strategy to study the genotype-by-environment interaction and identify novel traits for crop improvement from canopy to an organ level. Our aims were to study the phenotypic and metabolic traits that are related to grain yield and quality at canopy and organ levels, with a special focus on source-sink coordination under contrasting N supplies. Four modern durum wheat varieties with contrasting grain yield were grown in field conditions under two N fertilization levels in north-eastern Spain. We evaluated canopy vegetation indices taken throughout the growing season, physiological and metabolic traits in different photosynthetic organs (flag leaf blade, sheath, peduncle, awn, glume, and lemma) at anthesis and mid-grain filling stages, and agronomic and grain quality traits at harvest. Low N supply triggered an imbalance of C and N coordination at the whole plant level, leading to a reduction of grain yield and nutrient composition. The activities of key enzymes in C and N metabolism as well as the levels of photoassimilates showed that each organ plays an important role during grain filling, some with a higher photosynthetic capacity, others for nutrient storage for later stages of grain filling, or N assimilation and recycling. Interestingly, the enzyme activities and sucrose content of the ear organs were positively associated with grain yield and quality, suggesting, together with the regression models using isotope signatures, the potential contribution of these organs during grain filling. This study highlights the use of holistic approaches to the identification of novel targets to improve grain yield and quality in C(3) cereals and the key role of non-foliar organs at late-growth stages.
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spelling pubmed-91008082022-05-14 Source-Sink Dynamics in Field-Grown Durum Wheat Under Contrasting Nitrogen Supplies: Key Role of Non-Foliar Organs During Grain Filling Martínez-Peña, Raquel Schlereth, Armin Höhne, Melanie Encke, Beatrice Morcuende, Rosa Nieto-Taladriz, María Teresa Araus, José Luis Aparicio, Nieves Vicente, Rubén Front Plant Sci Plant Science The integration of high-throughput phenotyping and metabolic approaches is a suitable strategy to study the genotype-by-environment interaction and identify novel traits for crop improvement from canopy to an organ level. Our aims were to study the phenotypic and metabolic traits that are related to grain yield and quality at canopy and organ levels, with a special focus on source-sink coordination under contrasting N supplies. Four modern durum wheat varieties with contrasting grain yield were grown in field conditions under two N fertilization levels in north-eastern Spain. We evaluated canopy vegetation indices taken throughout the growing season, physiological and metabolic traits in different photosynthetic organs (flag leaf blade, sheath, peduncle, awn, glume, and lemma) at anthesis and mid-grain filling stages, and agronomic and grain quality traits at harvest. Low N supply triggered an imbalance of C and N coordination at the whole plant level, leading to a reduction of grain yield and nutrient composition. The activities of key enzymes in C and N metabolism as well as the levels of photoassimilates showed that each organ plays an important role during grain filling, some with a higher photosynthetic capacity, others for nutrient storage for later stages of grain filling, or N assimilation and recycling. Interestingly, the enzyme activities and sucrose content of the ear organs were positively associated with grain yield and quality, suggesting, together with the regression models using isotope signatures, the potential contribution of these organs during grain filling. This study highlights the use of holistic approaches to the identification of novel targets to improve grain yield and quality in C(3) cereals and the key role of non-foliar organs at late-growth stages. Frontiers Media S.A. 2022-04-29 /pmc/articles/PMC9100808/ /pubmed/35574116 http://dx.doi.org/10.3389/fpls.2022.869680 Text en Copyright © 2022 Martínez-Peña, Schlereth, Höhne, Encke, Morcuende, Nieto-Taladriz, Araus, Aparicio and Vicente. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Martínez-Peña, Raquel
Schlereth, Armin
Höhne, Melanie
Encke, Beatrice
Morcuende, Rosa
Nieto-Taladriz, María Teresa
Araus, José Luis
Aparicio, Nieves
Vicente, Rubén
Source-Sink Dynamics in Field-Grown Durum Wheat Under Contrasting Nitrogen Supplies: Key Role of Non-Foliar Organs During Grain Filling
title Source-Sink Dynamics in Field-Grown Durum Wheat Under Contrasting Nitrogen Supplies: Key Role of Non-Foliar Organs During Grain Filling
title_full Source-Sink Dynamics in Field-Grown Durum Wheat Under Contrasting Nitrogen Supplies: Key Role of Non-Foliar Organs During Grain Filling
title_fullStr Source-Sink Dynamics in Field-Grown Durum Wheat Under Contrasting Nitrogen Supplies: Key Role of Non-Foliar Organs During Grain Filling
title_full_unstemmed Source-Sink Dynamics in Field-Grown Durum Wheat Under Contrasting Nitrogen Supplies: Key Role of Non-Foliar Organs During Grain Filling
title_short Source-Sink Dynamics in Field-Grown Durum Wheat Under Contrasting Nitrogen Supplies: Key Role of Non-Foliar Organs During Grain Filling
title_sort source-sink dynamics in field-grown durum wheat under contrasting nitrogen supplies: key role of non-foliar organs during grain filling
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100808/
https://www.ncbi.nlm.nih.gov/pubmed/35574116
http://dx.doi.org/10.3389/fpls.2022.869680
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