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CBL-Interacting Protein Kinase OsCIPK18 Regulates the Response of Ammonium Toxicity in Rice Roots
Ammonium ([Formula: see text]) is one of the major nitrogen sources for plants. However, excessive ammonium can cause serious harm to the growth and development of plants, i.e., ammonium toxicity. The primary regulatory mechanisms behind ammonium toxicity are still poorly characterized. In this stud...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100847/ https://www.ncbi.nlm.nih.gov/pubmed/35574117 http://dx.doi.org/10.3389/fpls.2022.863283 |
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author | Sun, Tong Wang, Ting Qiang, Yalin Zhao, Gangqing Yang, Jian Zhong, Hua Peng, Xiaojue Yang, Jing Li, Yangsheng |
author_facet | Sun, Tong Wang, Ting Qiang, Yalin Zhao, Gangqing Yang, Jian Zhong, Hua Peng, Xiaojue Yang, Jing Li, Yangsheng |
author_sort | Sun, Tong |
collection | PubMed |
description | Ammonium ([Formula: see text]) is one of the major nitrogen sources for plants. However, excessive ammonium can cause serious harm to the growth and development of plants, i.e., ammonium toxicity. The primary regulatory mechanisms behind ammonium toxicity are still poorly characterized. In this study, we showed that OsCIPK18, a CBL-interacting protein kinase, plays an important role in response to ammonium toxicity by comparative analysis of the physiological and whole transcriptome of the T-DNA insertion mutant (cipk18) and the wild-type (WT). Root biomass and length of cipk18 are less inhibited by excess [Formula: see text] compared with WT, indicating increased resistance to ammonium toxicity. Transcriptome analysis reveals that OsCIPK18 affects the [Formula: see text] uptake by regulating the expression of OsAMT1;2 and other [Formula: see text] transporters, but does not affect ammonium assimilation. Differentially expressed genes induced by excess [Formula: see text] in WT and cipk18 were associated with functions, such as ion transport, metabolism, cell wall formation, and phytohormones signaling, suggesting a fundamental role for OsCIPK18 in ammonium toxicity. We further identified a transcriptional regulatory network downstream of OsCIPK18 under [Formula: see text] stress that is centered on several core transcription factors. Moreover, OsCIPK18 might function as a transmitter in the auxin and abscisic acid (ABA) signaling pathways affected by excess ammonium. These data allowed us to define an OsCIPK18-regulated/dependent transcriptomic network for the response of ammonium toxicity and provide new insights into the mechanisms underlying ammonium toxicity. |
format | Online Article Text |
id | pubmed-9100847 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-91008472022-05-14 CBL-Interacting Protein Kinase OsCIPK18 Regulates the Response of Ammonium Toxicity in Rice Roots Sun, Tong Wang, Ting Qiang, Yalin Zhao, Gangqing Yang, Jian Zhong, Hua Peng, Xiaojue Yang, Jing Li, Yangsheng Front Plant Sci Plant Science Ammonium ([Formula: see text]) is one of the major nitrogen sources for plants. However, excessive ammonium can cause serious harm to the growth and development of plants, i.e., ammonium toxicity. The primary regulatory mechanisms behind ammonium toxicity are still poorly characterized. In this study, we showed that OsCIPK18, a CBL-interacting protein kinase, plays an important role in response to ammonium toxicity by comparative analysis of the physiological and whole transcriptome of the T-DNA insertion mutant (cipk18) and the wild-type (WT). Root biomass and length of cipk18 are less inhibited by excess [Formula: see text] compared with WT, indicating increased resistance to ammonium toxicity. Transcriptome analysis reveals that OsCIPK18 affects the [Formula: see text] uptake by regulating the expression of OsAMT1;2 and other [Formula: see text] transporters, but does not affect ammonium assimilation. Differentially expressed genes induced by excess [Formula: see text] in WT and cipk18 were associated with functions, such as ion transport, metabolism, cell wall formation, and phytohormones signaling, suggesting a fundamental role for OsCIPK18 in ammonium toxicity. We further identified a transcriptional regulatory network downstream of OsCIPK18 under [Formula: see text] stress that is centered on several core transcription factors. Moreover, OsCIPK18 might function as a transmitter in the auxin and abscisic acid (ABA) signaling pathways affected by excess ammonium. These data allowed us to define an OsCIPK18-regulated/dependent transcriptomic network for the response of ammonium toxicity and provide new insights into the mechanisms underlying ammonium toxicity. Frontiers Media S.A. 2022-04-29 /pmc/articles/PMC9100847/ /pubmed/35574117 http://dx.doi.org/10.3389/fpls.2022.863283 Text en Copyright © 2022 Sun, Wang, Qiang, Zhao, Yang, Zhong, Peng, Yang and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Sun, Tong Wang, Ting Qiang, Yalin Zhao, Gangqing Yang, Jian Zhong, Hua Peng, Xiaojue Yang, Jing Li, Yangsheng CBL-Interacting Protein Kinase OsCIPK18 Regulates the Response of Ammonium Toxicity in Rice Roots |
title | CBL-Interacting Protein Kinase OsCIPK18 Regulates the Response of Ammonium Toxicity in Rice Roots |
title_full | CBL-Interacting Protein Kinase OsCIPK18 Regulates the Response of Ammonium Toxicity in Rice Roots |
title_fullStr | CBL-Interacting Protein Kinase OsCIPK18 Regulates the Response of Ammonium Toxicity in Rice Roots |
title_full_unstemmed | CBL-Interacting Protein Kinase OsCIPK18 Regulates the Response of Ammonium Toxicity in Rice Roots |
title_short | CBL-Interacting Protein Kinase OsCIPK18 Regulates the Response of Ammonium Toxicity in Rice Roots |
title_sort | cbl-interacting protein kinase oscipk18 regulates the response of ammonium toxicity in rice roots |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100847/ https://www.ncbi.nlm.nih.gov/pubmed/35574117 http://dx.doi.org/10.3389/fpls.2022.863283 |
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