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Non-Destructive Measurement of the Pumpkin Rootstock Root Phenotype Using AZURE KINECT
Rootstock grafting is an important method to improve the yield and quality of seedlings. Pumpkin is the rootstock of watermelon, melon, and cucumber, and the root phenotype of rootstock is an important reference for breeding. At present, the root phenotype is mainly measured by scanners, with which...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100892/ https://www.ncbi.nlm.nih.gov/pubmed/35567145 http://dx.doi.org/10.3390/plants11091144 |
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author | Zhang, Moran Xu, Shengyong Huang, Yuan Bie, Zhilong Notaguchi, Michitaka Zhou, Jingyi Wan, Xin Wang, Yuchen Dong, Wanjing |
author_facet | Zhang, Moran Xu, Shengyong Huang, Yuan Bie, Zhilong Notaguchi, Michitaka Zhou, Jingyi Wan, Xin Wang, Yuchen Dong, Wanjing |
author_sort | Zhang, Moran |
collection | PubMed |
description | Rootstock grafting is an important method to improve the yield and quality of seedlings. Pumpkin is the rootstock of watermelon, melon, and cucumber, and the root phenotype of rootstock is an important reference for breeding. At present, the root phenotype is mainly measured by scanners, with which it is difficult to achieve non-destructive and in situ measurements. In this work, we propose a method for non-destructive measurement of the root phenotype on the surface layer of the root ball of pumpkin rootstock plug seedlings and an accurate estimation of the surface area, length, and volume of total root using an AZURE KINECT sensor. Firstly, the KINECT is used to capture four-view color and depth images of the root surface, and then multi-view images are spliced to obtain a complete image of the root surface. After preprocessing of the images, we extract the roots from the root ball. For root phenotype measurements, the surface areas of the surface roots and root ball are calculated, followed by calculating root encapsulation. Next, the non-overlapping roots in the surface root image are extracted, and the ratio of the surface area to the skeleton length is used as the average diameter of total root. Based on the high correlation between the surface area of surface root and the surface area of total root, a linear fitting model is established to estimate the surface area, length, and volume of total root. The experiment ultimately showed that the measurement error for the average diameter of total root is less than 30 μm, and consistency with the scanner is higher than 93.3%. The accuracy of the surface area of total root estimation was found to be more than 88.1%, and the accuracy of the root length of total root estimation was observed to be greater than 87.2%. The method proposed in this paper offers similar accuracy to a scanner, which meets the needs of non-destructive root phenotype research. This method is expected to replace root scanners for high-throughput phenotypic measurements and provides a new avenue for root phenotype measurements of pumpkin rootstocks. This technology will provide key basic data for evaluating the root growth of pumpkin rootstocks. |
format | Online Article Text |
id | pubmed-9100892 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91008922022-05-14 Non-Destructive Measurement of the Pumpkin Rootstock Root Phenotype Using AZURE KINECT Zhang, Moran Xu, Shengyong Huang, Yuan Bie, Zhilong Notaguchi, Michitaka Zhou, Jingyi Wan, Xin Wang, Yuchen Dong, Wanjing Plants (Basel) Article Rootstock grafting is an important method to improve the yield and quality of seedlings. Pumpkin is the rootstock of watermelon, melon, and cucumber, and the root phenotype of rootstock is an important reference for breeding. At present, the root phenotype is mainly measured by scanners, with which it is difficult to achieve non-destructive and in situ measurements. In this work, we propose a method for non-destructive measurement of the root phenotype on the surface layer of the root ball of pumpkin rootstock plug seedlings and an accurate estimation of the surface area, length, and volume of total root using an AZURE KINECT sensor. Firstly, the KINECT is used to capture four-view color and depth images of the root surface, and then multi-view images are spliced to obtain a complete image of the root surface. After preprocessing of the images, we extract the roots from the root ball. For root phenotype measurements, the surface areas of the surface roots and root ball are calculated, followed by calculating root encapsulation. Next, the non-overlapping roots in the surface root image are extracted, and the ratio of the surface area to the skeleton length is used as the average diameter of total root. Based on the high correlation between the surface area of surface root and the surface area of total root, a linear fitting model is established to estimate the surface area, length, and volume of total root. The experiment ultimately showed that the measurement error for the average diameter of total root is less than 30 μm, and consistency with the scanner is higher than 93.3%. The accuracy of the surface area of total root estimation was found to be more than 88.1%, and the accuracy of the root length of total root estimation was observed to be greater than 87.2%. The method proposed in this paper offers similar accuracy to a scanner, which meets the needs of non-destructive root phenotype research. This method is expected to replace root scanners for high-throughput phenotypic measurements and provides a new avenue for root phenotype measurements of pumpkin rootstocks. This technology will provide key basic data for evaluating the root growth of pumpkin rootstocks. MDPI 2022-04-23 /pmc/articles/PMC9100892/ /pubmed/35567145 http://dx.doi.org/10.3390/plants11091144 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhang, Moran Xu, Shengyong Huang, Yuan Bie, Zhilong Notaguchi, Michitaka Zhou, Jingyi Wan, Xin Wang, Yuchen Dong, Wanjing Non-Destructive Measurement of the Pumpkin Rootstock Root Phenotype Using AZURE KINECT |
title | Non-Destructive Measurement of the Pumpkin Rootstock Root Phenotype Using AZURE KINECT |
title_full | Non-Destructive Measurement of the Pumpkin Rootstock Root Phenotype Using AZURE KINECT |
title_fullStr | Non-Destructive Measurement of the Pumpkin Rootstock Root Phenotype Using AZURE KINECT |
title_full_unstemmed | Non-Destructive Measurement of the Pumpkin Rootstock Root Phenotype Using AZURE KINECT |
title_short | Non-Destructive Measurement of the Pumpkin Rootstock Root Phenotype Using AZURE KINECT |
title_sort | non-destructive measurement of the pumpkin rootstock root phenotype using azure kinect |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100892/ https://www.ncbi.nlm.nih.gov/pubmed/35567145 http://dx.doi.org/10.3390/plants11091144 |
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