Microfluidics-Based Time-Resolved Fluorescence Immunoassay for the On-Site Detection of Aflatoxins B1 Zearalenone and Deoxynivalenol in Cereals

The primary pollutants in cereal products are aflatoxins B1 (AFB1), zearalenone (ZEN), and deoxynivalenol (DON). In this study, anti-AFB1 MAb (4C9), anti-ZEN MAb (2A3), and anti-DON MAb (1F10) were developed and used in time-resolved fluorescence immunoassay microfluidics to determine AFB1, ZEN, and DON in agricultural products. The linear range for AFB1, ZEN, and DON were 0.05~2.2 μg/kg, 1.45~375.75 μg/kg, and 11.1~124.2 μg/kg, respectively. In maize, the recoveries of AFB1/ZEN/DON were 92~101%, 102~105%, and 103~108%, respectively. High-performance liquid chromatography and the proposed approach had a good correlation. Time-resolved fluorescence immunoassay microfluidics is a highly efficient and sensitive field detection method for fungal toxins in agricultural products.