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The CaALAD Gene From Pepper (Capsicum annuum L.) Confers Chilling Stress Tolerance in Transgenic Arabidopsis Plants
The ALAD gene encodes an enzyme that is essential for chlorophyll biosynthesis and is involved in many other physiological processes in plants. In this study, the CaALAD gene was cloned from pepper and sequenced. Multiple sequence alignment and phylogenetic analysis of ALAD proteins from nine plant...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100946/ https://www.ncbi.nlm.nih.gov/pubmed/35574110 http://dx.doi.org/10.3389/fpls.2022.884990 |
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author | Wang, Huiping Liu, Zeci Xie, Jianming Li, Jing Zhang, Jing Yu, Jihua Hu, Linli Zhang, Guobin |
author_facet | Wang, Huiping Liu, Zeci Xie, Jianming Li, Jing Zhang, Jing Yu, Jihua Hu, Linli Zhang, Guobin |
author_sort | Wang, Huiping |
collection | PubMed |
description | The ALAD gene encodes an enzyme that is essential for chlorophyll biosynthesis and is involved in many other physiological processes in plants. In this study, the CaALAD gene was cloned from pepper and sequenced. Multiple sequence alignment and phylogenetic analysis of ALAD proteins from nine plant species showed that ALAD is highly conserved, and that CaALAD shows the highest homology with the ALAD protein from eggplant. Subcellular localization indicated that the CaALAD protein is mainly localized to the chloroplasts. After transferring CaALAD into the Arabidopsis thaliana genome, cold tolerance of the transgenic lines improved. Overexpression of CaALAD increased the relative transcription of the AtCBF2, AtICE1, and AtCOR15b genes in transgenic Arabidopsis plants exposed to low temperature (4°C) stress, and the contents of reactive oxygen species decreased due to increased activities of superoxide dismutase, peroxidase, and catalase. Moreover, chlorophyll biosynthesis, as determined by the contents of porphobilinogen, protoporphyrin IX, Mg-protoporphyrin IX, prochlorophyllate, and chlorophyll in the transgenic Arabidopsis plants, increased in response to low temperature stress. In addition, the transgenic lines were more sensitive to exogenous ALA and NaHS, and the H(2)S content of transgenic line plants increased more rapidly than in the wild-type, suggesting that CaALAD may respond to low temperatures by influencing the content of H(2)S, a signaling molecule. Our study gives a preliminary indication of the function of CaALAD and will provide a theoretical basis for future molecular breeding of cold tolerance in pepper. |
format | Online Article Text |
id | pubmed-9100946 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-91009462022-05-14 The CaALAD Gene From Pepper (Capsicum annuum L.) Confers Chilling Stress Tolerance in Transgenic Arabidopsis Plants Wang, Huiping Liu, Zeci Xie, Jianming Li, Jing Zhang, Jing Yu, Jihua Hu, Linli Zhang, Guobin Front Plant Sci Plant Science The ALAD gene encodes an enzyme that is essential for chlorophyll biosynthesis and is involved in many other physiological processes in plants. In this study, the CaALAD gene was cloned from pepper and sequenced. Multiple sequence alignment and phylogenetic analysis of ALAD proteins from nine plant species showed that ALAD is highly conserved, and that CaALAD shows the highest homology with the ALAD protein from eggplant. Subcellular localization indicated that the CaALAD protein is mainly localized to the chloroplasts. After transferring CaALAD into the Arabidopsis thaliana genome, cold tolerance of the transgenic lines improved. Overexpression of CaALAD increased the relative transcription of the AtCBF2, AtICE1, and AtCOR15b genes in transgenic Arabidopsis plants exposed to low temperature (4°C) stress, and the contents of reactive oxygen species decreased due to increased activities of superoxide dismutase, peroxidase, and catalase. Moreover, chlorophyll biosynthesis, as determined by the contents of porphobilinogen, protoporphyrin IX, Mg-protoporphyrin IX, prochlorophyllate, and chlorophyll in the transgenic Arabidopsis plants, increased in response to low temperature stress. In addition, the transgenic lines were more sensitive to exogenous ALA and NaHS, and the H(2)S content of transgenic line plants increased more rapidly than in the wild-type, suggesting that CaALAD may respond to low temperatures by influencing the content of H(2)S, a signaling molecule. Our study gives a preliminary indication of the function of CaALAD and will provide a theoretical basis for future molecular breeding of cold tolerance in pepper. Frontiers Media S.A. 2022-04-29 /pmc/articles/PMC9100946/ /pubmed/35574110 http://dx.doi.org/10.3389/fpls.2022.884990 Text en Copyright © 2022 Wang, Liu, Xie, Li, Zhang, Yu, Hu and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Wang, Huiping Liu, Zeci Xie, Jianming Li, Jing Zhang, Jing Yu, Jihua Hu, Linli Zhang, Guobin The CaALAD Gene From Pepper (Capsicum annuum L.) Confers Chilling Stress Tolerance in Transgenic Arabidopsis Plants |
title | The CaALAD Gene From Pepper (Capsicum annuum L.) Confers Chilling Stress Tolerance in Transgenic Arabidopsis Plants |
title_full | The CaALAD Gene From Pepper (Capsicum annuum L.) Confers Chilling Stress Tolerance in Transgenic Arabidopsis Plants |
title_fullStr | The CaALAD Gene From Pepper (Capsicum annuum L.) Confers Chilling Stress Tolerance in Transgenic Arabidopsis Plants |
title_full_unstemmed | The CaALAD Gene From Pepper (Capsicum annuum L.) Confers Chilling Stress Tolerance in Transgenic Arabidopsis Plants |
title_short | The CaALAD Gene From Pepper (Capsicum annuum L.) Confers Chilling Stress Tolerance in Transgenic Arabidopsis Plants |
title_sort | caalad gene from pepper (capsicum annuum l.) confers chilling stress tolerance in transgenic arabidopsis plants |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100946/ https://www.ncbi.nlm.nih.gov/pubmed/35574110 http://dx.doi.org/10.3389/fpls.2022.884990 |
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