Sex Differences in Mate Choice Preference Characteristics of Aequidens rivulatus

SIMPLE SUMMARY: Generally, animals prefer mating with partners of the opposite sex with specific features, which suggests that animals tend to choose mates with particular phenotypic traits. However, there are some differences in mate choice behavior and criteria between males and females. This study analyzed these differences between males and females in Aequidens rivulatus by quantifying body size, behavioral intention, and appearance. The results showed that males paid more attention to preference degree and female attractiveness, whereas females focused on ability and physical strength displays. Consequently, males who chose to mate were primarily associated with body size, behavioral intention, and appearance, whereas the preferences of females were body size, appearance, and behavioral intention. Collectively, our initial findings revealed that males and females have different criteria for mate choice, which is vital in determining successful mating and improving artificial mating. ABSTRACT: The mating roles of males and females, to a certain extent, are dynamic and variable. Several factors influence the mate choice process. Nonetheless, the main preference features have not yet been fully understood in Aequidens rivulatus. In this study, because of its natural pairing characteristics, A. rivulatus was selected to explore the mate choice preferences of different sexes. Specifically, male and female behavioral performances were described and quantified through a “no-choice paradigm” during mate choice. A total of 12 behavioral performances were defined in male mate choice (experiment 1), whereas 14 behavioral performances were defined in female mate choice (experiment 2). According to the obtained results, unselected females did not display any proactive behaviors in experiment 1, whereas unselected males exhibited proactive behaviors in experiment 2, including quivering, nipping, tail beating, swimming up and down, and aggression. It was also found that both male and female individuals tend to express dislike rather than like. Those behaviors with higher frequencies (e.g., quivering) often mean less energy expenditure, thus easier repeatability. Moreover, principal component analysis (PCA) was employed to extract and identify mate choice preference features. Preliminary results indicated that male preferences for a mate were mainly associated with body size, behavioral intention, and appearance, whereas the intensity of female preferences was in the order of body size, appearance, and behavioral intention. In addition, sex hormone levels were associated with mate choices.