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Endometrial Dysbiosis Is Related to Inflammatory Factors in Women with Repeated Implantation Failure: A Pilot Study

An abnormal endometrial microbiota has been suggested to impair the process of embryo implantation, thus leading to repeated implantation failure (RIF) in women undergoing in vitro fertilization (IVF). However, the molecular mechanisms linking uterine microbiota and IVF out-comes are still an open q...

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Autores principales: Cela, Vito, Daniele, Simona, Obino, Maria Elena Rosa, Ruggiero, Maria, Zappelli, Elisa, Ceccarelli, Lorenzo, Papini, Francesca, Marzi, Ilaria, Scarfò, Giorgia, Tosi, Fulvia, Franzoni, Ferdinando, Martini, Claudia, Artini, Paolo Giovanni
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9101226/
https://www.ncbi.nlm.nih.gov/pubmed/35566605
http://dx.doi.org/10.3390/jcm11092481
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author Cela, Vito
Daniele, Simona
Obino, Maria Elena Rosa
Ruggiero, Maria
Zappelli, Elisa
Ceccarelli, Lorenzo
Papini, Francesca
Marzi, Ilaria
Scarfò, Giorgia
Tosi, Fulvia
Franzoni, Ferdinando
Martini, Claudia
Artini, Paolo Giovanni
author_facet Cela, Vito
Daniele, Simona
Obino, Maria Elena Rosa
Ruggiero, Maria
Zappelli, Elisa
Ceccarelli, Lorenzo
Papini, Francesca
Marzi, Ilaria
Scarfò, Giorgia
Tosi, Fulvia
Franzoni, Ferdinando
Martini, Claudia
Artini, Paolo Giovanni
author_sort Cela, Vito
collection PubMed
description An abnormal endometrial microbiota has been suggested to impair the process of embryo implantation, thus leading to repeated implantation failure (RIF) in women undergoing in vitro fertilization (IVF). However, the molecular mechanisms linking uterine microbiota and IVF out-comes are still an open question. The aim of this cohort study was to outline the relationship between endometrial microbiota, inflammation and IVF outcomes. To this purpose, endometrial microbiota and selected components of the “cytokine network” were analyzed in women presenting RIF and divided between eubiosis and dysbiosis groups, according to the percentage of endometrial lactobacilli (≥90% or <90%, respectively). The Dysbiosis group presented significantly higher tissue concentrations of the inflammatory markers (IL-6, IL-1β, HIF-1α and COX-2) and significantly lower levels of the anti-inflammatory/well-being factors, IL-10 and IGF-1, with respect to women with eubiosis. Moreover, the Lactobacillus percentage was negatively related to the concentrations of the inflammatory molecules and positively related to IL-10/IGF-1. Interestingly, the number of IVF attempts was directly related to the levels of the inflammatory factors COX-2, IL-1β and HIF-1α in the eubiosis group. Overall, endometrial dysbiosis was demonstrated to be associated with inflammation-related endometrial changes affecting the process of embryo implantation, underlining the importance of assessing uterine microbiota in patients undergoing IVF.
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spelling pubmed-91012262022-05-14 Endometrial Dysbiosis Is Related to Inflammatory Factors in Women with Repeated Implantation Failure: A Pilot Study Cela, Vito Daniele, Simona Obino, Maria Elena Rosa Ruggiero, Maria Zappelli, Elisa Ceccarelli, Lorenzo Papini, Francesca Marzi, Ilaria Scarfò, Giorgia Tosi, Fulvia Franzoni, Ferdinando Martini, Claudia Artini, Paolo Giovanni J Clin Med Article An abnormal endometrial microbiota has been suggested to impair the process of embryo implantation, thus leading to repeated implantation failure (RIF) in women undergoing in vitro fertilization (IVF). However, the molecular mechanisms linking uterine microbiota and IVF out-comes are still an open question. The aim of this cohort study was to outline the relationship between endometrial microbiota, inflammation and IVF outcomes. To this purpose, endometrial microbiota and selected components of the “cytokine network” were analyzed in women presenting RIF and divided between eubiosis and dysbiosis groups, according to the percentage of endometrial lactobacilli (≥90% or <90%, respectively). The Dysbiosis group presented significantly higher tissue concentrations of the inflammatory markers (IL-6, IL-1β, HIF-1α and COX-2) and significantly lower levels of the anti-inflammatory/well-being factors, IL-10 and IGF-1, with respect to women with eubiosis. Moreover, the Lactobacillus percentage was negatively related to the concentrations of the inflammatory molecules and positively related to IL-10/IGF-1. Interestingly, the number of IVF attempts was directly related to the levels of the inflammatory factors COX-2, IL-1β and HIF-1α in the eubiosis group. Overall, endometrial dysbiosis was demonstrated to be associated with inflammation-related endometrial changes affecting the process of embryo implantation, underlining the importance of assessing uterine microbiota in patients undergoing IVF. MDPI 2022-04-28 /pmc/articles/PMC9101226/ /pubmed/35566605 http://dx.doi.org/10.3390/jcm11092481 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cela, Vito
Daniele, Simona
Obino, Maria Elena Rosa
Ruggiero, Maria
Zappelli, Elisa
Ceccarelli, Lorenzo
Papini, Francesca
Marzi, Ilaria
Scarfò, Giorgia
Tosi, Fulvia
Franzoni, Ferdinando
Martini, Claudia
Artini, Paolo Giovanni
Endometrial Dysbiosis Is Related to Inflammatory Factors in Women with Repeated Implantation Failure: A Pilot Study
title Endometrial Dysbiosis Is Related to Inflammatory Factors in Women with Repeated Implantation Failure: A Pilot Study
title_full Endometrial Dysbiosis Is Related to Inflammatory Factors in Women with Repeated Implantation Failure: A Pilot Study
title_fullStr Endometrial Dysbiosis Is Related to Inflammatory Factors in Women with Repeated Implantation Failure: A Pilot Study
title_full_unstemmed Endometrial Dysbiosis Is Related to Inflammatory Factors in Women with Repeated Implantation Failure: A Pilot Study
title_short Endometrial Dysbiosis Is Related to Inflammatory Factors in Women with Repeated Implantation Failure: A Pilot Study
title_sort endometrial dysbiosis is related to inflammatory factors in women with repeated implantation failure: a pilot study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9101226/
https://www.ncbi.nlm.nih.gov/pubmed/35566605
http://dx.doi.org/10.3390/jcm11092481
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