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Endometrial Dysbiosis Is Related to Inflammatory Factors in Women with Repeated Implantation Failure: A Pilot Study
An abnormal endometrial microbiota has been suggested to impair the process of embryo implantation, thus leading to repeated implantation failure (RIF) in women undergoing in vitro fertilization (IVF). However, the molecular mechanisms linking uterine microbiota and IVF out-comes are still an open q...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9101226/ https://www.ncbi.nlm.nih.gov/pubmed/35566605 http://dx.doi.org/10.3390/jcm11092481 |
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author | Cela, Vito Daniele, Simona Obino, Maria Elena Rosa Ruggiero, Maria Zappelli, Elisa Ceccarelli, Lorenzo Papini, Francesca Marzi, Ilaria Scarfò, Giorgia Tosi, Fulvia Franzoni, Ferdinando Martini, Claudia Artini, Paolo Giovanni |
author_facet | Cela, Vito Daniele, Simona Obino, Maria Elena Rosa Ruggiero, Maria Zappelli, Elisa Ceccarelli, Lorenzo Papini, Francesca Marzi, Ilaria Scarfò, Giorgia Tosi, Fulvia Franzoni, Ferdinando Martini, Claudia Artini, Paolo Giovanni |
author_sort | Cela, Vito |
collection | PubMed |
description | An abnormal endometrial microbiota has been suggested to impair the process of embryo implantation, thus leading to repeated implantation failure (RIF) in women undergoing in vitro fertilization (IVF). However, the molecular mechanisms linking uterine microbiota and IVF out-comes are still an open question. The aim of this cohort study was to outline the relationship between endometrial microbiota, inflammation and IVF outcomes. To this purpose, endometrial microbiota and selected components of the “cytokine network” were analyzed in women presenting RIF and divided between eubiosis and dysbiosis groups, according to the percentage of endometrial lactobacilli (≥90% or <90%, respectively). The Dysbiosis group presented significantly higher tissue concentrations of the inflammatory markers (IL-6, IL-1β, HIF-1α and COX-2) and significantly lower levels of the anti-inflammatory/well-being factors, IL-10 and IGF-1, with respect to women with eubiosis. Moreover, the Lactobacillus percentage was negatively related to the concentrations of the inflammatory molecules and positively related to IL-10/IGF-1. Interestingly, the number of IVF attempts was directly related to the levels of the inflammatory factors COX-2, IL-1β and HIF-1α in the eubiosis group. Overall, endometrial dysbiosis was demonstrated to be associated with inflammation-related endometrial changes affecting the process of embryo implantation, underlining the importance of assessing uterine microbiota in patients undergoing IVF. |
format | Online Article Text |
id | pubmed-9101226 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91012262022-05-14 Endometrial Dysbiosis Is Related to Inflammatory Factors in Women with Repeated Implantation Failure: A Pilot Study Cela, Vito Daniele, Simona Obino, Maria Elena Rosa Ruggiero, Maria Zappelli, Elisa Ceccarelli, Lorenzo Papini, Francesca Marzi, Ilaria Scarfò, Giorgia Tosi, Fulvia Franzoni, Ferdinando Martini, Claudia Artini, Paolo Giovanni J Clin Med Article An abnormal endometrial microbiota has been suggested to impair the process of embryo implantation, thus leading to repeated implantation failure (RIF) in women undergoing in vitro fertilization (IVF). However, the molecular mechanisms linking uterine microbiota and IVF out-comes are still an open question. The aim of this cohort study was to outline the relationship between endometrial microbiota, inflammation and IVF outcomes. To this purpose, endometrial microbiota and selected components of the “cytokine network” were analyzed in women presenting RIF and divided between eubiosis and dysbiosis groups, according to the percentage of endometrial lactobacilli (≥90% or <90%, respectively). The Dysbiosis group presented significantly higher tissue concentrations of the inflammatory markers (IL-6, IL-1β, HIF-1α and COX-2) and significantly lower levels of the anti-inflammatory/well-being factors, IL-10 and IGF-1, with respect to women with eubiosis. Moreover, the Lactobacillus percentage was negatively related to the concentrations of the inflammatory molecules and positively related to IL-10/IGF-1. Interestingly, the number of IVF attempts was directly related to the levels of the inflammatory factors COX-2, IL-1β and HIF-1α in the eubiosis group. Overall, endometrial dysbiosis was demonstrated to be associated with inflammation-related endometrial changes affecting the process of embryo implantation, underlining the importance of assessing uterine microbiota in patients undergoing IVF. MDPI 2022-04-28 /pmc/articles/PMC9101226/ /pubmed/35566605 http://dx.doi.org/10.3390/jcm11092481 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cela, Vito Daniele, Simona Obino, Maria Elena Rosa Ruggiero, Maria Zappelli, Elisa Ceccarelli, Lorenzo Papini, Francesca Marzi, Ilaria Scarfò, Giorgia Tosi, Fulvia Franzoni, Ferdinando Martini, Claudia Artini, Paolo Giovanni Endometrial Dysbiosis Is Related to Inflammatory Factors in Women with Repeated Implantation Failure: A Pilot Study |
title | Endometrial Dysbiosis Is Related to Inflammatory Factors in Women with Repeated Implantation Failure: A Pilot Study |
title_full | Endometrial Dysbiosis Is Related to Inflammatory Factors in Women with Repeated Implantation Failure: A Pilot Study |
title_fullStr | Endometrial Dysbiosis Is Related to Inflammatory Factors in Women with Repeated Implantation Failure: A Pilot Study |
title_full_unstemmed | Endometrial Dysbiosis Is Related to Inflammatory Factors in Women with Repeated Implantation Failure: A Pilot Study |
title_short | Endometrial Dysbiosis Is Related to Inflammatory Factors in Women with Repeated Implantation Failure: A Pilot Study |
title_sort | endometrial dysbiosis is related to inflammatory factors in women with repeated implantation failure: a pilot study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9101226/ https://www.ncbi.nlm.nih.gov/pubmed/35566605 http://dx.doi.org/10.3390/jcm11092481 |
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