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References Values of Soluble α-Klotho Serum Levels Using an Enzyme-Linked Immunosorbent Assay in Healthy Adults Aged 18–85 Years

α-Klotho protein is a powerful predictor of the aging process and lifespan. Although lowered circulating soluble α-Klotho levels have been observed in aged non-healthy individuals, no specific reference values across a wide range of ages and sex using an enzyme-linked immunosorbent assay (ELISA) are...

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Autores principales: Espuch-Oliver, Andrea, Vázquez-Lorente, Héctor, Jurado-Fasoli, Lucas, de Haro-Muñoz, Tomás, Díaz-Alberola, Irene, López-Velez, María del Señor, de Haro-Romero, Teresa, Castillo, Manuel J., Amaro-Gahete, Francisco J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9101232/
https://www.ncbi.nlm.nih.gov/pubmed/35566540
http://dx.doi.org/10.3390/jcm11092415
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author Espuch-Oliver, Andrea
Vázquez-Lorente, Héctor
Jurado-Fasoli, Lucas
de Haro-Muñoz, Tomás
Díaz-Alberola, Irene
López-Velez, María del Señor
de Haro-Romero, Teresa
Castillo, Manuel J.
Amaro-Gahete, Francisco J.
author_facet Espuch-Oliver, Andrea
Vázquez-Lorente, Héctor
Jurado-Fasoli, Lucas
de Haro-Muñoz, Tomás
Díaz-Alberola, Irene
López-Velez, María del Señor
de Haro-Romero, Teresa
Castillo, Manuel J.
Amaro-Gahete, Francisco J.
author_sort Espuch-Oliver, Andrea
collection PubMed
description α-Klotho protein is a powerful predictor of the aging process and lifespan. Although lowered circulating soluble α-Klotho levels have been observed in aged non-healthy individuals, no specific reference values across a wide range of ages and sex using an enzyme-linked immunosorbent assay (ELISA) are available for larger cohorts of healthy individuals. The present analytical cross-sectional study was aimed to establish the reference values of soluble α-Klotho serum levels in healthy adults by age and sex groups. A total of 346 (59% women) healthy individuals aged from 18 to 85 years were recruited. Subjects were divided by sex and age as: (i) young (18–34.9 years), (ii) middle-aged (35–54.9 years), and (iii) senior (55–85 years) individuals. The soluble α-Klotho levels were measured in serum using ELISA. Senior adults were the age-group that presented the lowest soluble α-Klotho serum levels (p < 0.01), with age showing a negative association with soluble α-Klotho serum levels (p < 0.001). No differences between sexes were observed. Therefore, soluble α-Klotho levels were especially decreased—regardless of sex—in our cohort of healthy individuals because of the physiological decline derived from the aging process. We recommend routine assessments of soluble α-Klotho levels using ELISA as a simple and cheap detectable marker of aging that improves quality of life in the elderly.
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spelling pubmed-91012322022-05-14 References Values of Soluble α-Klotho Serum Levels Using an Enzyme-Linked Immunosorbent Assay in Healthy Adults Aged 18–85 Years Espuch-Oliver, Andrea Vázquez-Lorente, Héctor Jurado-Fasoli, Lucas de Haro-Muñoz, Tomás Díaz-Alberola, Irene López-Velez, María del Señor de Haro-Romero, Teresa Castillo, Manuel J. Amaro-Gahete, Francisco J. J Clin Med Article α-Klotho protein is a powerful predictor of the aging process and lifespan. Although lowered circulating soluble α-Klotho levels have been observed in aged non-healthy individuals, no specific reference values across a wide range of ages and sex using an enzyme-linked immunosorbent assay (ELISA) are available for larger cohorts of healthy individuals. The present analytical cross-sectional study was aimed to establish the reference values of soluble α-Klotho serum levels in healthy adults by age and sex groups. A total of 346 (59% women) healthy individuals aged from 18 to 85 years were recruited. Subjects were divided by sex and age as: (i) young (18–34.9 years), (ii) middle-aged (35–54.9 years), and (iii) senior (55–85 years) individuals. The soluble α-Klotho levels were measured in serum using ELISA. Senior adults were the age-group that presented the lowest soluble α-Klotho serum levels (p < 0.01), with age showing a negative association with soluble α-Klotho serum levels (p < 0.001). No differences between sexes were observed. Therefore, soluble α-Klotho levels were especially decreased—regardless of sex—in our cohort of healthy individuals because of the physiological decline derived from the aging process. We recommend routine assessments of soluble α-Klotho levels using ELISA as a simple and cheap detectable marker of aging that improves quality of life in the elderly. MDPI 2022-04-25 /pmc/articles/PMC9101232/ /pubmed/35566540 http://dx.doi.org/10.3390/jcm11092415 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Espuch-Oliver, Andrea
Vázquez-Lorente, Héctor
Jurado-Fasoli, Lucas
de Haro-Muñoz, Tomás
Díaz-Alberola, Irene
López-Velez, María del Señor
de Haro-Romero, Teresa
Castillo, Manuel J.
Amaro-Gahete, Francisco J.
References Values of Soluble α-Klotho Serum Levels Using an Enzyme-Linked Immunosorbent Assay in Healthy Adults Aged 18–85 Years
title References Values of Soluble α-Klotho Serum Levels Using an Enzyme-Linked Immunosorbent Assay in Healthy Adults Aged 18–85 Years
title_full References Values of Soluble α-Klotho Serum Levels Using an Enzyme-Linked Immunosorbent Assay in Healthy Adults Aged 18–85 Years
title_fullStr References Values of Soluble α-Klotho Serum Levels Using an Enzyme-Linked Immunosorbent Assay in Healthy Adults Aged 18–85 Years
title_full_unstemmed References Values of Soluble α-Klotho Serum Levels Using an Enzyme-Linked Immunosorbent Assay in Healthy Adults Aged 18–85 Years
title_short References Values of Soluble α-Klotho Serum Levels Using an Enzyme-Linked Immunosorbent Assay in Healthy Adults Aged 18–85 Years
title_sort references values of soluble α-klotho serum levels using an enzyme-linked immunosorbent assay in healthy adults aged 18–85 years
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9101232/
https://www.ncbi.nlm.nih.gov/pubmed/35566540
http://dx.doi.org/10.3390/jcm11092415
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