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References Values of Soluble α-Klotho Serum Levels Using an Enzyme-Linked Immunosorbent Assay in Healthy Adults Aged 18–85 Years
α-Klotho protein is a powerful predictor of the aging process and lifespan. Although lowered circulating soluble α-Klotho levels have been observed in aged non-healthy individuals, no specific reference values across a wide range of ages and sex using an enzyme-linked immunosorbent assay (ELISA) are...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9101232/ https://www.ncbi.nlm.nih.gov/pubmed/35566540 http://dx.doi.org/10.3390/jcm11092415 |
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author | Espuch-Oliver, Andrea Vázquez-Lorente, Héctor Jurado-Fasoli, Lucas de Haro-Muñoz, Tomás Díaz-Alberola, Irene López-Velez, María del Señor de Haro-Romero, Teresa Castillo, Manuel J. Amaro-Gahete, Francisco J. |
author_facet | Espuch-Oliver, Andrea Vázquez-Lorente, Héctor Jurado-Fasoli, Lucas de Haro-Muñoz, Tomás Díaz-Alberola, Irene López-Velez, María del Señor de Haro-Romero, Teresa Castillo, Manuel J. Amaro-Gahete, Francisco J. |
author_sort | Espuch-Oliver, Andrea |
collection | PubMed |
description | α-Klotho protein is a powerful predictor of the aging process and lifespan. Although lowered circulating soluble α-Klotho levels have been observed in aged non-healthy individuals, no specific reference values across a wide range of ages and sex using an enzyme-linked immunosorbent assay (ELISA) are available for larger cohorts of healthy individuals. The present analytical cross-sectional study was aimed to establish the reference values of soluble α-Klotho serum levels in healthy adults by age and sex groups. A total of 346 (59% women) healthy individuals aged from 18 to 85 years were recruited. Subjects were divided by sex and age as: (i) young (18–34.9 years), (ii) middle-aged (35–54.9 years), and (iii) senior (55–85 years) individuals. The soluble α-Klotho levels were measured in serum using ELISA. Senior adults were the age-group that presented the lowest soluble α-Klotho serum levels (p < 0.01), with age showing a negative association with soluble α-Klotho serum levels (p < 0.001). No differences between sexes were observed. Therefore, soluble α-Klotho levels were especially decreased—regardless of sex—in our cohort of healthy individuals because of the physiological decline derived from the aging process. We recommend routine assessments of soluble α-Klotho levels using ELISA as a simple and cheap detectable marker of aging that improves quality of life in the elderly. |
format | Online Article Text |
id | pubmed-9101232 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91012322022-05-14 References Values of Soluble α-Klotho Serum Levels Using an Enzyme-Linked Immunosorbent Assay in Healthy Adults Aged 18–85 Years Espuch-Oliver, Andrea Vázquez-Lorente, Héctor Jurado-Fasoli, Lucas de Haro-Muñoz, Tomás Díaz-Alberola, Irene López-Velez, María del Señor de Haro-Romero, Teresa Castillo, Manuel J. Amaro-Gahete, Francisco J. J Clin Med Article α-Klotho protein is a powerful predictor of the aging process and lifespan. Although lowered circulating soluble α-Klotho levels have been observed in aged non-healthy individuals, no specific reference values across a wide range of ages and sex using an enzyme-linked immunosorbent assay (ELISA) are available for larger cohorts of healthy individuals. The present analytical cross-sectional study was aimed to establish the reference values of soluble α-Klotho serum levels in healthy adults by age and sex groups. A total of 346 (59% women) healthy individuals aged from 18 to 85 years were recruited. Subjects were divided by sex and age as: (i) young (18–34.9 years), (ii) middle-aged (35–54.9 years), and (iii) senior (55–85 years) individuals. The soluble α-Klotho levels were measured in serum using ELISA. Senior adults were the age-group that presented the lowest soluble α-Klotho serum levels (p < 0.01), with age showing a negative association with soluble α-Klotho serum levels (p < 0.001). No differences between sexes were observed. Therefore, soluble α-Klotho levels were especially decreased—regardless of sex—in our cohort of healthy individuals because of the physiological decline derived from the aging process. We recommend routine assessments of soluble α-Klotho levels using ELISA as a simple and cheap detectable marker of aging that improves quality of life in the elderly. MDPI 2022-04-25 /pmc/articles/PMC9101232/ /pubmed/35566540 http://dx.doi.org/10.3390/jcm11092415 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Espuch-Oliver, Andrea Vázquez-Lorente, Héctor Jurado-Fasoli, Lucas de Haro-Muñoz, Tomás Díaz-Alberola, Irene López-Velez, María del Señor de Haro-Romero, Teresa Castillo, Manuel J. Amaro-Gahete, Francisco J. References Values of Soluble α-Klotho Serum Levels Using an Enzyme-Linked Immunosorbent Assay in Healthy Adults Aged 18–85 Years |
title | References Values of Soluble α-Klotho Serum Levels Using an Enzyme-Linked Immunosorbent Assay in Healthy Adults Aged 18–85 Years |
title_full | References Values of Soluble α-Klotho Serum Levels Using an Enzyme-Linked Immunosorbent Assay in Healthy Adults Aged 18–85 Years |
title_fullStr | References Values of Soluble α-Klotho Serum Levels Using an Enzyme-Linked Immunosorbent Assay in Healthy Adults Aged 18–85 Years |
title_full_unstemmed | References Values of Soluble α-Klotho Serum Levels Using an Enzyme-Linked Immunosorbent Assay in Healthy Adults Aged 18–85 Years |
title_short | References Values of Soluble α-Klotho Serum Levels Using an Enzyme-Linked Immunosorbent Assay in Healthy Adults Aged 18–85 Years |
title_sort | references values of soluble α-klotho serum levels using an enzyme-linked immunosorbent assay in healthy adults aged 18–85 years |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9101232/ https://www.ncbi.nlm.nih.gov/pubmed/35566540 http://dx.doi.org/10.3390/jcm11092415 |
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