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Bacillus amyloliquefaciens Enriched Camel Milk Attenuated Colitis Symptoms in Mice Model

Fermented camel’s milk has various health beneficial prebiotics and probiotics. This study aimed to evaluate the preventive efficacy of Bacillus amyloliquefaciens enriched camel milk (BEY) in 2-, 4- and 6-Trinitrobenzenesulfonic acid (TNBS)-induced colitis mice models. To this end, the immune modula...

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Autores principales: Khalifa, Ashraf, Sheikh, Abdullah, Ibrahim, Hairul Islam Mohamed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9101272/
https://www.ncbi.nlm.nih.gov/pubmed/35565934
http://dx.doi.org/10.3390/nu14091967
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author Khalifa, Ashraf
Sheikh, Abdullah
Ibrahim, Hairul Islam Mohamed
author_facet Khalifa, Ashraf
Sheikh, Abdullah
Ibrahim, Hairul Islam Mohamed
author_sort Khalifa, Ashraf
collection PubMed
description Fermented camel’s milk has various health beneficial prebiotics and probiotics. This study aimed to evaluate the preventive efficacy of Bacillus amyloliquefaciens enriched camel milk (BEY) in 2-, 4- and 6-Trinitrobenzenesulfonic acid (TNBS)-induced colitis mice models. To this end, the immune modulatory effects of Bacillus amyloliquefaciens (BA) on TNF-α challenged HT29 colon cells were estimated using the cell proliferation and cytokines ELISA method. BEY was prepared using the incubation method and nutritional value was quantified by comparing it to commercial yogurt. Furthermore, TNBS-induced colitis was established and the level of disease index, pathological scores, and inflammatory markers of BEY-treated mice using macroscopic and microscopic examinations, qPCR and immunoblot were investigated. The results demonstrate that BA is non-toxic to HT29 colon cells and balanced the inflammatory cytokines. BEY reduced the colitis disease index, and improved the body weight and colon length of the TNBS-induced mice. Additionally, Myeloperoxidase (MPO) and pro-inflammatory cytokines (IL1β, IL6, IL8 and TNF-α) were attenuated by BEY treatment. Moreover, the inflammatory progress mRNA and protein markers nuclear factor kappa B (NFκB), phosphatase and tensin homolog (PTEN), proliferating cell nuclear antigen (PCNA), cyclooxygenase-2 (COX-2) and occludin were significantly down-regulated by BEY treatment. Interestingly, significant suppression of PCNA was observed in colonic tissues using the immunohistochemical examination. Treatment with BEY increased the epigenetic (microRNA217) interactions with PCNA. In conclusion, the BEY clearly alleviated the colitis symptoms and in the future could be used to formulate a probiotic-based diet for the host gut health and control the inflammatory bowel syndrome in mammals.
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spelling pubmed-91012722022-05-14 Bacillus amyloliquefaciens Enriched Camel Milk Attenuated Colitis Symptoms in Mice Model Khalifa, Ashraf Sheikh, Abdullah Ibrahim, Hairul Islam Mohamed Nutrients Article Fermented camel’s milk has various health beneficial prebiotics and probiotics. This study aimed to evaluate the preventive efficacy of Bacillus amyloliquefaciens enriched camel milk (BEY) in 2-, 4- and 6-Trinitrobenzenesulfonic acid (TNBS)-induced colitis mice models. To this end, the immune modulatory effects of Bacillus amyloliquefaciens (BA) on TNF-α challenged HT29 colon cells were estimated using the cell proliferation and cytokines ELISA method. BEY was prepared using the incubation method and nutritional value was quantified by comparing it to commercial yogurt. Furthermore, TNBS-induced colitis was established and the level of disease index, pathological scores, and inflammatory markers of BEY-treated mice using macroscopic and microscopic examinations, qPCR and immunoblot were investigated. The results demonstrate that BA is non-toxic to HT29 colon cells and balanced the inflammatory cytokines. BEY reduced the colitis disease index, and improved the body weight and colon length of the TNBS-induced mice. Additionally, Myeloperoxidase (MPO) and pro-inflammatory cytokines (IL1β, IL6, IL8 and TNF-α) were attenuated by BEY treatment. Moreover, the inflammatory progress mRNA and protein markers nuclear factor kappa B (NFκB), phosphatase and tensin homolog (PTEN), proliferating cell nuclear antigen (PCNA), cyclooxygenase-2 (COX-2) and occludin were significantly down-regulated by BEY treatment. Interestingly, significant suppression of PCNA was observed in colonic tissues using the immunohistochemical examination. Treatment with BEY increased the epigenetic (microRNA217) interactions with PCNA. In conclusion, the BEY clearly alleviated the colitis symptoms and in the future could be used to formulate a probiotic-based diet for the host gut health and control the inflammatory bowel syndrome in mammals. MDPI 2022-05-08 /pmc/articles/PMC9101272/ /pubmed/35565934 http://dx.doi.org/10.3390/nu14091967 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Khalifa, Ashraf
Sheikh, Abdullah
Ibrahim, Hairul Islam Mohamed
Bacillus amyloliquefaciens Enriched Camel Milk Attenuated Colitis Symptoms in Mice Model
title Bacillus amyloliquefaciens Enriched Camel Milk Attenuated Colitis Symptoms in Mice Model
title_full Bacillus amyloliquefaciens Enriched Camel Milk Attenuated Colitis Symptoms in Mice Model
title_fullStr Bacillus amyloliquefaciens Enriched Camel Milk Attenuated Colitis Symptoms in Mice Model
title_full_unstemmed Bacillus amyloliquefaciens Enriched Camel Milk Attenuated Colitis Symptoms in Mice Model
title_short Bacillus amyloliquefaciens Enriched Camel Milk Attenuated Colitis Symptoms in Mice Model
title_sort bacillus amyloliquefaciens enriched camel milk attenuated colitis symptoms in mice model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9101272/
https://www.ncbi.nlm.nih.gov/pubmed/35565934
http://dx.doi.org/10.3390/nu14091967
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