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Reducing Endogenous Labile Zn May Help to Reduce Smooth Muscle Cell Injury around Vascular Stents

Vascular stent service involves complex service environments and performance requirements, among which the histocompatibility of the stent could seriously affect the therapeutic effect. In the pathology of vascular disease, the thin fiber cap is easily ruptured, exposing the necrotic core below, and...

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Autores principales: Zeng, Zheng, Xie, Yinhong, Li, Li, Wang, Huanran, Tan, Jianying, Li, Xia, Bian, Qihao, Zhang, Yu, Liu, Tao, Weng, Yajun, Chen, Junying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9101291/
https://www.ncbi.nlm.nih.gov/pubmed/35563532
http://dx.doi.org/10.3390/ijms23095139
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author Zeng, Zheng
Xie, Yinhong
Li, Li
Wang, Huanran
Tan, Jianying
Li, Xia
Bian, Qihao
Zhang, Yu
Liu, Tao
Weng, Yajun
Chen, Junying
author_facet Zeng, Zheng
Xie, Yinhong
Li, Li
Wang, Huanran
Tan, Jianying
Li, Xia
Bian, Qihao
Zhang, Yu
Liu, Tao
Weng, Yajun
Chen, Junying
author_sort Zeng, Zheng
collection PubMed
description Vascular stent service involves complex service environments and performance requirements, among which the histocompatibility of the stent could seriously affect the therapeutic effect. In the pathology of vascular disease, the thin fiber cap is easily ruptured, exposing the necrotic core below, and triggering a series of dangerous biochemical reactions. In contrast, the thin neointima, considered an essential structure growing on the stent, may evolve into vulnerable plaque structures due to lesions induced by the stent. Therefore, the reduction of necrosis around the stent below the thin neointima is indispensable. In this work, different cell model experiments suggested that the content of endogenous labile Zn positively correlated with cell injury. Zinquin-Zn fluorescence experiments and zinc ion channels research suggested that the change in the content of endogenous labile Zn in smooth muscle cells is affected by different stent coatings. The content of endogenous labile Zn in cells negatively correlated with cell viability. Animal experiments indirectly verified the increase in endogenous labile Zn by detecting the expression of Zn regulatory protein (metallothionein) in the necrotic tissues. Reducing the content of endogenous labile Zn may favor a reduction in smooth muscle cell injury and necrosis. This biochemical mechanism is effective in improving the therapeutic effect of vascular stents.
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spelling pubmed-91012912022-05-14 Reducing Endogenous Labile Zn May Help to Reduce Smooth Muscle Cell Injury around Vascular Stents Zeng, Zheng Xie, Yinhong Li, Li Wang, Huanran Tan, Jianying Li, Xia Bian, Qihao Zhang, Yu Liu, Tao Weng, Yajun Chen, Junying Int J Mol Sci Article Vascular stent service involves complex service environments and performance requirements, among which the histocompatibility of the stent could seriously affect the therapeutic effect. In the pathology of vascular disease, the thin fiber cap is easily ruptured, exposing the necrotic core below, and triggering a series of dangerous biochemical reactions. In contrast, the thin neointima, considered an essential structure growing on the stent, may evolve into vulnerable plaque structures due to lesions induced by the stent. Therefore, the reduction of necrosis around the stent below the thin neointima is indispensable. In this work, different cell model experiments suggested that the content of endogenous labile Zn positively correlated with cell injury. Zinquin-Zn fluorescence experiments and zinc ion channels research suggested that the change in the content of endogenous labile Zn in smooth muscle cells is affected by different stent coatings. The content of endogenous labile Zn in cells negatively correlated with cell viability. Animal experiments indirectly verified the increase in endogenous labile Zn by detecting the expression of Zn regulatory protein (metallothionein) in the necrotic tissues. Reducing the content of endogenous labile Zn may favor a reduction in smooth muscle cell injury and necrosis. This biochemical mechanism is effective in improving the therapeutic effect of vascular stents. MDPI 2022-05-05 /pmc/articles/PMC9101291/ /pubmed/35563532 http://dx.doi.org/10.3390/ijms23095139 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zeng, Zheng
Xie, Yinhong
Li, Li
Wang, Huanran
Tan, Jianying
Li, Xia
Bian, Qihao
Zhang, Yu
Liu, Tao
Weng, Yajun
Chen, Junying
Reducing Endogenous Labile Zn May Help to Reduce Smooth Muscle Cell Injury around Vascular Stents
title Reducing Endogenous Labile Zn May Help to Reduce Smooth Muscle Cell Injury around Vascular Stents
title_full Reducing Endogenous Labile Zn May Help to Reduce Smooth Muscle Cell Injury around Vascular Stents
title_fullStr Reducing Endogenous Labile Zn May Help to Reduce Smooth Muscle Cell Injury around Vascular Stents
title_full_unstemmed Reducing Endogenous Labile Zn May Help to Reduce Smooth Muscle Cell Injury around Vascular Stents
title_short Reducing Endogenous Labile Zn May Help to Reduce Smooth Muscle Cell Injury around Vascular Stents
title_sort reducing endogenous labile zn may help to reduce smooth muscle cell injury around vascular stents
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9101291/
https://www.ncbi.nlm.nih.gov/pubmed/35563532
http://dx.doi.org/10.3390/ijms23095139
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