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Genomic Mutations of BK Polyomavirus in Patients after Kidney Transplantation: A Cross-Sectional Study in Vietnam
Objectives: The purpose of this study was to identify the SNP sites and determine the BKV genotype circulating in kidney-transplant Vietnamese recipients based on the VP1 gene region. Methods: 344 samples were collected from post-kidney-transplant recipients at the 103 Vietnam Military Hospital to i...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9101345/ https://www.ncbi.nlm.nih.gov/pubmed/35566670 http://dx.doi.org/10.3390/jcm11092544 |
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author | Kien, Truong Quy Toan, Pham Quoc Nghia, Phan Ba Van, Diem Thi Duc, Nguyen Van Ha, Do Manh Dung, Nguyen Thi Thuy Ha, Nguyen Thi Thu Quyen, Le Thi Bao Vinh, Hoang Trung Manh, Bui Van Su, Hoang Xuan Tien, Tran Viet Thang, Le Viet Rostaing, Lionel |
author_facet | Kien, Truong Quy Toan, Pham Quoc Nghia, Phan Ba Van, Diem Thi Duc, Nguyen Van Ha, Do Manh Dung, Nguyen Thi Thuy Ha, Nguyen Thi Thu Quyen, Le Thi Bao Vinh, Hoang Trung Manh, Bui Van Su, Hoang Xuan Tien, Tran Viet Thang, Le Viet Rostaing, Lionel |
author_sort | Kien, Truong Quy |
collection | PubMed |
description | Objectives: The purpose of this study was to identify the SNP sites and determine the BKV genotype circulating in kidney-transplant Vietnamese recipients based on the VP1 gene region. Methods: 344 samples were collected from post-kidney-transplant recipients at the 103 Vietnam Military Hospital to investigate the number of BKV infections. Positive samples with a sufficient virus concentration were analyzed by nested PCR in the VP1 region, sequencing detected genotyping and single-nucleotide polymorphism. Results: BKV infection was determined in 214 patients (62.2%), of whom 11 (5.1%) were diagnosed with BKV-associated nephropathy. Among the 90 BKV-I strains sequenced, 89 (98.88%) were strains of I/b-1 and 1 (1.12%) was strain I/b-2. The 60 BKV-IV strains had a greater diversity of subgroups, including 40% IV/a-1, 1.66% IV/a-2, 56.68% IV/c-1, and 1.16% IV/c-2. Additionally, of 11 cases diagnosed with BKVN, seven belonged to subgroup I/b-1 (63.6%) and four to subgroup IV/c-1 (36.4%). Moreover, 22 specific SNPs that were genotype I or IV were determined in this Vietnamese population. Specifically, at position 1745, for the Vietnamese BKV-IV strains, the SNP position (A→G) appeared in 57/60 samples (95%). This causes transformation of the amino acid N→S. This SNP site can enable detection of genotype IV in Vietnam. It represents a unique evolution pattern and mutation that has not been found in other international strains. Conclusion: The BKV-I genotype was more common than BKV-IV; however, mutations that occur on the VP1 typing region of BKV-IV strains were more frequent than in BKV-I strains. |
format | Online Article Text |
id | pubmed-9101345 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91013452022-05-14 Genomic Mutations of BK Polyomavirus in Patients after Kidney Transplantation: A Cross-Sectional Study in Vietnam Kien, Truong Quy Toan, Pham Quoc Nghia, Phan Ba Van, Diem Thi Duc, Nguyen Van Ha, Do Manh Dung, Nguyen Thi Thuy Ha, Nguyen Thi Thu Quyen, Le Thi Bao Vinh, Hoang Trung Manh, Bui Van Su, Hoang Xuan Tien, Tran Viet Thang, Le Viet Rostaing, Lionel J Clin Med Article Objectives: The purpose of this study was to identify the SNP sites and determine the BKV genotype circulating in kidney-transplant Vietnamese recipients based on the VP1 gene region. Methods: 344 samples were collected from post-kidney-transplant recipients at the 103 Vietnam Military Hospital to investigate the number of BKV infections. Positive samples with a sufficient virus concentration were analyzed by nested PCR in the VP1 region, sequencing detected genotyping and single-nucleotide polymorphism. Results: BKV infection was determined in 214 patients (62.2%), of whom 11 (5.1%) were diagnosed with BKV-associated nephropathy. Among the 90 BKV-I strains sequenced, 89 (98.88%) were strains of I/b-1 and 1 (1.12%) was strain I/b-2. The 60 BKV-IV strains had a greater diversity of subgroups, including 40% IV/a-1, 1.66% IV/a-2, 56.68% IV/c-1, and 1.16% IV/c-2. Additionally, of 11 cases diagnosed with BKVN, seven belonged to subgroup I/b-1 (63.6%) and four to subgroup IV/c-1 (36.4%). Moreover, 22 specific SNPs that were genotype I or IV were determined in this Vietnamese population. Specifically, at position 1745, for the Vietnamese BKV-IV strains, the SNP position (A→G) appeared in 57/60 samples (95%). This causes transformation of the amino acid N→S. This SNP site can enable detection of genotype IV in Vietnam. It represents a unique evolution pattern and mutation that has not been found in other international strains. Conclusion: The BKV-I genotype was more common than BKV-IV; however, mutations that occur on the VP1 typing region of BKV-IV strains were more frequent than in BKV-I strains. MDPI 2022-05-01 /pmc/articles/PMC9101345/ /pubmed/35566670 http://dx.doi.org/10.3390/jcm11092544 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kien, Truong Quy Toan, Pham Quoc Nghia, Phan Ba Van, Diem Thi Duc, Nguyen Van Ha, Do Manh Dung, Nguyen Thi Thuy Ha, Nguyen Thi Thu Quyen, Le Thi Bao Vinh, Hoang Trung Manh, Bui Van Su, Hoang Xuan Tien, Tran Viet Thang, Le Viet Rostaing, Lionel Genomic Mutations of BK Polyomavirus in Patients after Kidney Transplantation: A Cross-Sectional Study in Vietnam |
title | Genomic Mutations of BK Polyomavirus in Patients after Kidney Transplantation: A Cross-Sectional Study in Vietnam |
title_full | Genomic Mutations of BK Polyomavirus in Patients after Kidney Transplantation: A Cross-Sectional Study in Vietnam |
title_fullStr | Genomic Mutations of BK Polyomavirus in Patients after Kidney Transplantation: A Cross-Sectional Study in Vietnam |
title_full_unstemmed | Genomic Mutations of BK Polyomavirus in Patients after Kidney Transplantation: A Cross-Sectional Study in Vietnam |
title_short | Genomic Mutations of BK Polyomavirus in Patients after Kidney Transplantation: A Cross-Sectional Study in Vietnam |
title_sort | genomic mutations of bk polyomavirus in patients after kidney transplantation: a cross-sectional study in vietnam |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9101345/ https://www.ncbi.nlm.nih.gov/pubmed/35566670 http://dx.doi.org/10.3390/jcm11092544 |
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