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Genomic Mutations of BK Polyomavirus in Patients after Kidney Transplantation: A Cross-Sectional Study in Vietnam

Objectives: The purpose of this study was to identify the SNP sites and determine the BKV genotype circulating in kidney-transplant Vietnamese recipients based on the VP1 gene region. Methods: 344 samples were collected from post-kidney-transplant recipients at the 103 Vietnam Military Hospital to i...

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Autores principales: Kien, Truong Quy, Toan, Pham Quoc, Nghia, Phan Ba, Van, Diem Thi, Duc, Nguyen Van, Ha, Do Manh, Dung, Nguyen Thi Thuy, Ha, Nguyen Thi Thu, Quyen, Le Thi Bao, Vinh, Hoang Trung, Manh, Bui Van, Su, Hoang Xuan, Tien, Tran Viet, Thang, Le Viet, Rostaing, Lionel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9101345/
https://www.ncbi.nlm.nih.gov/pubmed/35566670
http://dx.doi.org/10.3390/jcm11092544
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author Kien, Truong Quy
Toan, Pham Quoc
Nghia, Phan Ba
Van, Diem Thi
Duc, Nguyen Van
Ha, Do Manh
Dung, Nguyen Thi Thuy
Ha, Nguyen Thi Thu
Quyen, Le Thi Bao
Vinh, Hoang Trung
Manh, Bui Van
Su, Hoang Xuan
Tien, Tran Viet
Thang, Le Viet
Rostaing, Lionel
author_facet Kien, Truong Quy
Toan, Pham Quoc
Nghia, Phan Ba
Van, Diem Thi
Duc, Nguyen Van
Ha, Do Manh
Dung, Nguyen Thi Thuy
Ha, Nguyen Thi Thu
Quyen, Le Thi Bao
Vinh, Hoang Trung
Manh, Bui Van
Su, Hoang Xuan
Tien, Tran Viet
Thang, Le Viet
Rostaing, Lionel
author_sort Kien, Truong Quy
collection PubMed
description Objectives: The purpose of this study was to identify the SNP sites and determine the BKV genotype circulating in kidney-transplant Vietnamese recipients based on the VP1 gene region. Methods: 344 samples were collected from post-kidney-transplant recipients at the 103 Vietnam Military Hospital to investigate the number of BKV infections. Positive samples with a sufficient virus concentration were analyzed by nested PCR in the VP1 region, sequencing detected genotyping and single-nucleotide polymorphism. Results: BKV infection was determined in 214 patients (62.2%), of whom 11 (5.1%) were diagnosed with BKV-associated nephropathy. Among the 90 BKV-I strains sequenced, 89 (98.88%) were strains of I/b-1 and 1 (1.12%) was strain I/b-2. The 60 BKV-IV strains had a greater diversity of subgroups, including 40% IV/a-1, 1.66% IV/a-2, 56.68% IV/c-1, and 1.16% IV/c-2. Additionally, of 11 cases diagnosed with BKVN, seven belonged to subgroup I/b-1 (63.6%) and four to subgroup IV/c-1 (36.4%). Moreover, 22 specific SNPs that were genotype I or IV were determined in this Vietnamese population. Specifically, at position 1745, for the Vietnamese BKV-IV strains, the SNP position (A→G) appeared in 57/60 samples (95%). This causes transformation of the amino acid N→S. This SNP site can enable detection of genotype IV in Vietnam. It represents a unique evolution pattern and mutation that has not been found in other international strains. Conclusion: The BKV-I genotype was more common than BKV-IV; however, mutations that occur on the VP1 typing region of BKV-IV strains were more frequent than in BKV-I strains.
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spelling pubmed-91013452022-05-14 Genomic Mutations of BK Polyomavirus in Patients after Kidney Transplantation: A Cross-Sectional Study in Vietnam Kien, Truong Quy Toan, Pham Quoc Nghia, Phan Ba Van, Diem Thi Duc, Nguyen Van Ha, Do Manh Dung, Nguyen Thi Thuy Ha, Nguyen Thi Thu Quyen, Le Thi Bao Vinh, Hoang Trung Manh, Bui Van Su, Hoang Xuan Tien, Tran Viet Thang, Le Viet Rostaing, Lionel J Clin Med Article Objectives: The purpose of this study was to identify the SNP sites and determine the BKV genotype circulating in kidney-transplant Vietnamese recipients based on the VP1 gene region. Methods: 344 samples were collected from post-kidney-transplant recipients at the 103 Vietnam Military Hospital to investigate the number of BKV infections. Positive samples with a sufficient virus concentration were analyzed by nested PCR in the VP1 region, sequencing detected genotyping and single-nucleotide polymorphism. Results: BKV infection was determined in 214 patients (62.2%), of whom 11 (5.1%) were diagnosed with BKV-associated nephropathy. Among the 90 BKV-I strains sequenced, 89 (98.88%) were strains of I/b-1 and 1 (1.12%) was strain I/b-2. The 60 BKV-IV strains had a greater diversity of subgroups, including 40% IV/a-1, 1.66% IV/a-2, 56.68% IV/c-1, and 1.16% IV/c-2. Additionally, of 11 cases diagnosed with BKVN, seven belonged to subgroup I/b-1 (63.6%) and four to subgroup IV/c-1 (36.4%). Moreover, 22 specific SNPs that were genotype I or IV were determined in this Vietnamese population. Specifically, at position 1745, for the Vietnamese BKV-IV strains, the SNP position (A→G) appeared in 57/60 samples (95%). This causes transformation of the amino acid N→S. This SNP site can enable detection of genotype IV in Vietnam. It represents a unique evolution pattern and mutation that has not been found in other international strains. Conclusion: The BKV-I genotype was more common than BKV-IV; however, mutations that occur on the VP1 typing region of BKV-IV strains were more frequent than in BKV-I strains. MDPI 2022-05-01 /pmc/articles/PMC9101345/ /pubmed/35566670 http://dx.doi.org/10.3390/jcm11092544 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kien, Truong Quy
Toan, Pham Quoc
Nghia, Phan Ba
Van, Diem Thi
Duc, Nguyen Van
Ha, Do Manh
Dung, Nguyen Thi Thuy
Ha, Nguyen Thi Thu
Quyen, Le Thi Bao
Vinh, Hoang Trung
Manh, Bui Van
Su, Hoang Xuan
Tien, Tran Viet
Thang, Le Viet
Rostaing, Lionel
Genomic Mutations of BK Polyomavirus in Patients after Kidney Transplantation: A Cross-Sectional Study in Vietnam
title Genomic Mutations of BK Polyomavirus in Patients after Kidney Transplantation: A Cross-Sectional Study in Vietnam
title_full Genomic Mutations of BK Polyomavirus in Patients after Kidney Transplantation: A Cross-Sectional Study in Vietnam
title_fullStr Genomic Mutations of BK Polyomavirus in Patients after Kidney Transplantation: A Cross-Sectional Study in Vietnam
title_full_unstemmed Genomic Mutations of BK Polyomavirus in Patients after Kidney Transplantation: A Cross-Sectional Study in Vietnam
title_short Genomic Mutations of BK Polyomavirus in Patients after Kidney Transplantation: A Cross-Sectional Study in Vietnam
title_sort genomic mutations of bk polyomavirus in patients after kidney transplantation: a cross-sectional study in vietnam
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9101345/
https://www.ncbi.nlm.nih.gov/pubmed/35566670
http://dx.doi.org/10.3390/jcm11092544
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