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HIV-2 Neutralization Sensitivity in Relation to Co-Receptor Entry Pathways and Env Motifs
HIV-2, compared to HIV-1, elicits potent and broadly neutralizing antibodies, and uses a broad range of co-receptors. However, both sensitivity to neutralization and breadth of co-receptor use varies between HIV-2 isolates, and the molecular background is still not fully understood. Thus, in the cur...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9101540/ https://www.ncbi.nlm.nih.gov/pubmed/35563157 http://dx.doi.org/10.3390/ijms23094766 |
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author | Szojka, Zsófia Ilona Karlson, Sara Johansson, Emil Şahin, Gülşen Özkaya Jansson, Marianne |
author_facet | Szojka, Zsófia Ilona Karlson, Sara Johansson, Emil Şahin, Gülşen Özkaya Jansson, Marianne |
author_sort | Szojka, Zsófia Ilona |
collection | PubMed |
description | HIV-2, compared to HIV-1, elicits potent and broadly neutralizing antibodies, and uses a broad range of co-receptors. However, both sensitivity to neutralization and breadth of co-receptor use varies between HIV-2 isolates, and the molecular background is still not fully understood. Thus, in the current study, we have deciphered relationships between HIV-2 neutralization sensitivity, co-receptor use and viral envelope glycoprotein (Env) molecular motifs. A panel of primary HIV-2 isolates, with predefined use of co-receptors, was assessed for neutralization sensitivity using a set of HIV-2 Env-directed monoclonal antibodies and co-receptor indicator cell lines. Neutralization sensitivity of the isolates was analysed in relation target cell co-receptor expression, in addition to amino acid motifs and predicted structures of Env regions. Results showed that HIV-2 isolates were more resistant to neutralizing antibodies when entering target cells via the alternative co-receptor GPR15, as compared to CCR5. A similar pattern was noted for isolates using the alternative co-receptor CXCR6. Sensitivity to neutralizing antibodies appeared also to be linked to specific Env motifs in V1/V2 and C3 regions. Our findings suggest that HIV-2 sensitivity to neutralization depends both on which co-receptor is used for cell entry and on specific Env motifs. This study highlights the multifactorial mechanisms behind HIV-2 neutralization sensitivity. |
format | Online Article Text |
id | pubmed-9101540 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91015402022-05-14 HIV-2 Neutralization Sensitivity in Relation to Co-Receptor Entry Pathways and Env Motifs Szojka, Zsófia Ilona Karlson, Sara Johansson, Emil Şahin, Gülşen Özkaya Jansson, Marianne Int J Mol Sci Article HIV-2, compared to HIV-1, elicits potent and broadly neutralizing antibodies, and uses a broad range of co-receptors. However, both sensitivity to neutralization and breadth of co-receptor use varies between HIV-2 isolates, and the molecular background is still not fully understood. Thus, in the current study, we have deciphered relationships between HIV-2 neutralization sensitivity, co-receptor use and viral envelope glycoprotein (Env) molecular motifs. A panel of primary HIV-2 isolates, with predefined use of co-receptors, was assessed for neutralization sensitivity using a set of HIV-2 Env-directed monoclonal antibodies and co-receptor indicator cell lines. Neutralization sensitivity of the isolates was analysed in relation target cell co-receptor expression, in addition to amino acid motifs and predicted structures of Env regions. Results showed that HIV-2 isolates were more resistant to neutralizing antibodies when entering target cells via the alternative co-receptor GPR15, as compared to CCR5. A similar pattern was noted for isolates using the alternative co-receptor CXCR6. Sensitivity to neutralizing antibodies appeared also to be linked to specific Env motifs in V1/V2 and C3 regions. Our findings suggest that HIV-2 sensitivity to neutralization depends both on which co-receptor is used for cell entry and on specific Env motifs. This study highlights the multifactorial mechanisms behind HIV-2 neutralization sensitivity. MDPI 2022-04-26 /pmc/articles/PMC9101540/ /pubmed/35563157 http://dx.doi.org/10.3390/ijms23094766 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Szojka, Zsófia Ilona Karlson, Sara Johansson, Emil Şahin, Gülşen Özkaya Jansson, Marianne HIV-2 Neutralization Sensitivity in Relation to Co-Receptor Entry Pathways and Env Motifs |
title | HIV-2 Neutralization Sensitivity in Relation to Co-Receptor Entry Pathways and Env Motifs |
title_full | HIV-2 Neutralization Sensitivity in Relation to Co-Receptor Entry Pathways and Env Motifs |
title_fullStr | HIV-2 Neutralization Sensitivity in Relation to Co-Receptor Entry Pathways and Env Motifs |
title_full_unstemmed | HIV-2 Neutralization Sensitivity in Relation to Co-Receptor Entry Pathways and Env Motifs |
title_short | HIV-2 Neutralization Sensitivity in Relation to Co-Receptor Entry Pathways and Env Motifs |
title_sort | hiv-2 neutralization sensitivity in relation to co-receptor entry pathways and env motifs |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9101540/ https://www.ncbi.nlm.nih.gov/pubmed/35563157 http://dx.doi.org/10.3390/ijms23094766 |
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