Cargando…

Transcriptional Contribution of Transposable Elements in Relation to Salinity Conditions in Teleosts and Silencing Mechanisms Involved

Fish are an interesting taxon comprising species adapted to a wide range of environments. In this work, we analyzed the transcriptional contribution of transposable elements (TEs) in the gill transcriptomes of three fish species exposed to different salinity conditions. We considered the giant marbl...

Descripción completa

Detalles Bibliográficos
Autores principales: Carotti, Elisa, Carducci, Federica, Greco, Samuele, Gerdol, Marco, Di Marino, Daniele, Perta, Nunzio, La Teana, Anna, Canapa, Adriana, Barucca, Marco, Biscotti, Maria Assunta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9101882/
https://www.ncbi.nlm.nih.gov/pubmed/35563606
http://dx.doi.org/10.3390/ijms23095215
_version_ 1784707195645984768
author Carotti, Elisa
Carducci, Federica
Greco, Samuele
Gerdol, Marco
Di Marino, Daniele
Perta, Nunzio
La Teana, Anna
Canapa, Adriana
Barucca, Marco
Biscotti, Maria Assunta
author_facet Carotti, Elisa
Carducci, Federica
Greco, Samuele
Gerdol, Marco
Di Marino, Daniele
Perta, Nunzio
La Teana, Anna
Canapa, Adriana
Barucca, Marco
Biscotti, Maria Assunta
author_sort Carotti, Elisa
collection PubMed
description Fish are an interesting taxon comprising species adapted to a wide range of environments. In this work, we analyzed the transcriptional contribution of transposable elements (TEs) in the gill transcriptomes of three fish species exposed to different salinity conditions. We considered the giant marbled eel Anguilla marmorata and the chum salmon Oncorhynchus keta, both diadromous, and the marine medaka Oryzias melastigma, an euryhaline organism sensu stricto. Our analyses revealed an interesting activity of TEs in the case of juvenile eels, commonly adapted to salty water, when exposed to brackish and freshwater conditions. Moreover, the expression assessment of genes involved in TE silencing mechanisms (six in heterochromatin formation, fourteen known to be part of the nucleosome remodeling deacetylase (NuRD) complex, and four of the Argonaute subfamily) unveiled that they are active. Finally, our results evidenced for the first time a krüppel-associated box (KRAB)-like domain specific to actinopterygians that, together with TRIM33, might allow the functioning of NuRD complex also in fish species. The possible interaction between these two proteins was supported by structural prediction analyses.
format Online
Article
Text
id pubmed-9101882
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-91018822022-05-14 Transcriptional Contribution of Transposable Elements in Relation to Salinity Conditions in Teleosts and Silencing Mechanisms Involved Carotti, Elisa Carducci, Federica Greco, Samuele Gerdol, Marco Di Marino, Daniele Perta, Nunzio La Teana, Anna Canapa, Adriana Barucca, Marco Biscotti, Maria Assunta Int J Mol Sci Article Fish are an interesting taxon comprising species adapted to a wide range of environments. In this work, we analyzed the transcriptional contribution of transposable elements (TEs) in the gill transcriptomes of three fish species exposed to different salinity conditions. We considered the giant marbled eel Anguilla marmorata and the chum salmon Oncorhynchus keta, both diadromous, and the marine medaka Oryzias melastigma, an euryhaline organism sensu stricto. Our analyses revealed an interesting activity of TEs in the case of juvenile eels, commonly adapted to salty water, when exposed to brackish and freshwater conditions. Moreover, the expression assessment of genes involved in TE silencing mechanisms (six in heterochromatin formation, fourteen known to be part of the nucleosome remodeling deacetylase (NuRD) complex, and four of the Argonaute subfamily) unveiled that they are active. Finally, our results evidenced for the first time a krüppel-associated box (KRAB)-like domain specific to actinopterygians that, together with TRIM33, might allow the functioning of NuRD complex also in fish species. The possible interaction between these two proteins was supported by structural prediction analyses. MDPI 2022-05-06 /pmc/articles/PMC9101882/ /pubmed/35563606 http://dx.doi.org/10.3390/ijms23095215 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Carotti, Elisa
Carducci, Federica
Greco, Samuele
Gerdol, Marco
Di Marino, Daniele
Perta, Nunzio
La Teana, Anna
Canapa, Adriana
Barucca, Marco
Biscotti, Maria Assunta
Transcriptional Contribution of Transposable Elements in Relation to Salinity Conditions in Teleosts and Silencing Mechanisms Involved
title Transcriptional Contribution of Transposable Elements in Relation to Salinity Conditions in Teleosts and Silencing Mechanisms Involved
title_full Transcriptional Contribution of Transposable Elements in Relation to Salinity Conditions in Teleosts and Silencing Mechanisms Involved
title_fullStr Transcriptional Contribution of Transposable Elements in Relation to Salinity Conditions in Teleosts and Silencing Mechanisms Involved
title_full_unstemmed Transcriptional Contribution of Transposable Elements in Relation to Salinity Conditions in Teleosts and Silencing Mechanisms Involved
title_short Transcriptional Contribution of Transposable Elements in Relation to Salinity Conditions in Teleosts and Silencing Mechanisms Involved
title_sort transcriptional contribution of transposable elements in relation to salinity conditions in teleosts and silencing mechanisms involved
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9101882/
https://www.ncbi.nlm.nih.gov/pubmed/35563606
http://dx.doi.org/10.3390/ijms23095215
work_keys_str_mv AT carottielisa transcriptionalcontributionoftransposableelementsinrelationtosalinityconditionsinteleostsandsilencingmechanismsinvolved
AT carduccifederica transcriptionalcontributionoftransposableelementsinrelationtosalinityconditionsinteleostsandsilencingmechanismsinvolved
AT grecosamuele transcriptionalcontributionoftransposableelementsinrelationtosalinityconditionsinteleostsandsilencingmechanismsinvolved
AT gerdolmarco transcriptionalcontributionoftransposableelementsinrelationtosalinityconditionsinteleostsandsilencingmechanismsinvolved
AT dimarinodaniele transcriptionalcontributionoftransposableelementsinrelationtosalinityconditionsinteleostsandsilencingmechanismsinvolved
AT pertanunzio transcriptionalcontributionoftransposableelementsinrelationtosalinityconditionsinteleostsandsilencingmechanismsinvolved
AT lateanaanna transcriptionalcontributionoftransposableelementsinrelationtosalinityconditionsinteleostsandsilencingmechanismsinvolved
AT canapaadriana transcriptionalcontributionoftransposableelementsinrelationtosalinityconditionsinteleostsandsilencingmechanismsinvolved
AT baruccamarco transcriptionalcontributionoftransposableelementsinrelationtosalinityconditionsinteleostsandsilencingmechanismsinvolved
AT biscottimariaassunta transcriptionalcontributionoftransposableelementsinrelationtosalinityconditionsinteleostsandsilencingmechanismsinvolved