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How CAR T Cells Breathe
The manufacture of efficacious CAR T cells represents a major challenge in cellular therapy. An important aspect of their quality concerns energy production and consumption, known as metabolism. T cells tend to adopt diverse metabolic profiles depending on their differentiation state and their stimu...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9102061/ https://www.ncbi.nlm.nih.gov/pubmed/35563759 http://dx.doi.org/10.3390/cells11091454 |
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author | Forcados, Christopher Joaquina, Sandy Casey, Nicholas Paul Caulier, Benjamin Wälchli, Sébastien |
author_facet | Forcados, Christopher Joaquina, Sandy Casey, Nicholas Paul Caulier, Benjamin Wälchli, Sébastien |
author_sort | Forcados, Christopher |
collection | PubMed |
description | The manufacture of efficacious CAR T cells represents a major challenge in cellular therapy. An important aspect of their quality concerns energy production and consumption, known as metabolism. T cells tend to adopt diverse metabolic profiles depending on their differentiation state and their stimulation level. It is therefore expected that the introduction of a synthetic molecule such as CAR, activating endogenous signaling pathways, will affect metabolism. In addition, upon patient treatment, the tumor microenvironment might influence the CAR T cell metabolism by compromising the energy resources. The access to novel technology with higher throughput and reduced cost has led to an increased interest in studying metabolism. Indeed, methods to quantify glycolysis and mitochondrial respiration have been available for decades but were rarely applied in the context of CAR T cell therapy before the release of the Seahorse XF apparatus. The present review will focus on the use of this instrument in the context of studies describing the impact of CAR on T cell metabolism and the strategies to render of CAR T cells more metabolically fit. |
format | Online Article Text |
id | pubmed-9102061 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91020612022-05-14 How CAR T Cells Breathe Forcados, Christopher Joaquina, Sandy Casey, Nicholas Paul Caulier, Benjamin Wälchli, Sébastien Cells Review The manufacture of efficacious CAR T cells represents a major challenge in cellular therapy. An important aspect of their quality concerns energy production and consumption, known as metabolism. T cells tend to adopt diverse metabolic profiles depending on their differentiation state and their stimulation level. It is therefore expected that the introduction of a synthetic molecule such as CAR, activating endogenous signaling pathways, will affect metabolism. In addition, upon patient treatment, the tumor microenvironment might influence the CAR T cell metabolism by compromising the energy resources. The access to novel technology with higher throughput and reduced cost has led to an increased interest in studying metabolism. Indeed, methods to quantify glycolysis and mitochondrial respiration have been available for decades but were rarely applied in the context of CAR T cell therapy before the release of the Seahorse XF apparatus. The present review will focus on the use of this instrument in the context of studies describing the impact of CAR on T cell metabolism and the strategies to render of CAR T cells more metabolically fit. MDPI 2022-04-25 /pmc/articles/PMC9102061/ /pubmed/35563759 http://dx.doi.org/10.3390/cells11091454 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Forcados, Christopher Joaquina, Sandy Casey, Nicholas Paul Caulier, Benjamin Wälchli, Sébastien How CAR T Cells Breathe |
title | How CAR T Cells Breathe |
title_full | How CAR T Cells Breathe |
title_fullStr | How CAR T Cells Breathe |
title_full_unstemmed | How CAR T Cells Breathe |
title_short | How CAR T Cells Breathe |
title_sort | how car t cells breathe |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9102061/ https://www.ncbi.nlm.nih.gov/pubmed/35563759 http://dx.doi.org/10.3390/cells11091454 |
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