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A New Phenylazo-Based Fluorescent Probe for Sensitive Detection of Hypochlorous Acid in Aqueous Solution
In this paper, we designed and synthesized a novel phenylazo-based fluorescent probe (RHN) for the sensing and imaging of hypochlorous acid (HClO) in mitochondria in living cells. In this process, HClO promoted the oxidation of the phenylazo group to generate a free Rhodol fluorophore moiety, which...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9102862/ https://www.ncbi.nlm.nih.gov/pubmed/35566328 http://dx.doi.org/10.3390/molecules27092978 |
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author | Liu, Qiuchen Liu, Chang He, Song Zhao, Liancheng Zeng, Xianshun Zhou, Jin Gong, Jin |
author_facet | Liu, Qiuchen Liu, Chang He, Song Zhao, Liancheng Zeng, Xianshun Zhou, Jin Gong, Jin |
author_sort | Liu, Qiuchen |
collection | PubMed |
description | In this paper, we designed and synthesized a novel phenylazo-based fluorescent probe (RHN) for the sensing and imaging of hypochlorous acid (HClO) in mitochondria in living cells. In this process, HClO promoted the oxidation of the phenylazo group to generate a free Rhodol fluorophore moiety, which in turn restored strong fluorescence and realized the detection of HClO. As expected, RHN exhibited high selectivity, high sensitivity and rapid response, with detection limits as low as 22 nM (1.155 ng/mL). Importantly, the results of the cell imaging experiments indicated that RHN has the ability to image and sense HClO in mitochondria, which is of great significance for exploration of the specific role of HClO in both the immune system and diseases. |
format | Online Article Text |
id | pubmed-9102862 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91028622022-05-14 A New Phenylazo-Based Fluorescent Probe for Sensitive Detection of Hypochlorous Acid in Aqueous Solution Liu, Qiuchen Liu, Chang He, Song Zhao, Liancheng Zeng, Xianshun Zhou, Jin Gong, Jin Molecules Article In this paper, we designed and synthesized a novel phenylazo-based fluorescent probe (RHN) for the sensing and imaging of hypochlorous acid (HClO) in mitochondria in living cells. In this process, HClO promoted the oxidation of the phenylazo group to generate a free Rhodol fluorophore moiety, which in turn restored strong fluorescence and realized the detection of HClO. As expected, RHN exhibited high selectivity, high sensitivity and rapid response, with detection limits as low as 22 nM (1.155 ng/mL). Importantly, the results of the cell imaging experiments indicated that RHN has the ability to image and sense HClO in mitochondria, which is of great significance for exploration of the specific role of HClO in both the immune system and diseases. MDPI 2022-05-06 /pmc/articles/PMC9102862/ /pubmed/35566328 http://dx.doi.org/10.3390/molecules27092978 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Liu, Qiuchen Liu, Chang He, Song Zhao, Liancheng Zeng, Xianshun Zhou, Jin Gong, Jin A New Phenylazo-Based Fluorescent Probe for Sensitive Detection of Hypochlorous Acid in Aqueous Solution |
title | A New Phenylazo-Based Fluorescent Probe for Sensitive Detection of Hypochlorous Acid in Aqueous Solution |
title_full | A New Phenylazo-Based Fluorescent Probe for Sensitive Detection of Hypochlorous Acid in Aqueous Solution |
title_fullStr | A New Phenylazo-Based Fluorescent Probe for Sensitive Detection of Hypochlorous Acid in Aqueous Solution |
title_full_unstemmed | A New Phenylazo-Based Fluorescent Probe for Sensitive Detection of Hypochlorous Acid in Aqueous Solution |
title_short | A New Phenylazo-Based Fluorescent Probe for Sensitive Detection of Hypochlorous Acid in Aqueous Solution |
title_sort | new phenylazo-based fluorescent probe for sensitive detection of hypochlorous acid in aqueous solution |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9102862/ https://www.ncbi.nlm.nih.gov/pubmed/35566328 http://dx.doi.org/10.3390/molecules27092978 |
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