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A Single-Cell Raman Spectroscopy Analysis of Bone Marrow Mesenchymal Stem/Stromal Cells to Identify Inter-Individual Diversity

The heterogeneity of stem cells represents the main challenge in regenerative medicine development. This issue is particularly pronounced when it comes to the use of primary mesenchymal stem/stromal cells (MSCs) due to a lack of identification markers. Considering the need for additional approaches...

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Autores principales: Kukolj, Tamara, Lazarević, Jasmina, Borojević, Ana, Ralević, Uroš, Vujić, Dragana, Jauković, Aleksandra, Lazarević, Nenad, Bugarski, Diana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9103070/
https://www.ncbi.nlm.nih.gov/pubmed/35563306
http://dx.doi.org/10.3390/ijms23094915
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author Kukolj, Tamara
Lazarević, Jasmina
Borojević, Ana
Ralević, Uroš
Vujić, Dragana
Jauković, Aleksandra
Lazarević, Nenad
Bugarski, Diana
author_facet Kukolj, Tamara
Lazarević, Jasmina
Borojević, Ana
Ralević, Uroš
Vujić, Dragana
Jauković, Aleksandra
Lazarević, Nenad
Bugarski, Diana
author_sort Kukolj, Tamara
collection PubMed
description The heterogeneity of stem cells represents the main challenge in regenerative medicine development. This issue is particularly pronounced when it comes to the use of primary mesenchymal stem/stromal cells (MSCs) due to a lack of identification markers. Considering the need for additional approaches in MSCs characterization, we applied Raman spectroscopy to investigate inter-individual differences between bone marrow MSCs (BM-MSCs). Based on standard biological tests, BM-MSCs of analyzed donors fulfill all conditions for their characterization, while no donor-related specifics were observed in terms of BM-MSCs morphology, phenotype, multilineage differentiation potential, colony-forming capacity, expression of pluripotency-associated markers or proliferative capacity. However, examination of BM-MSCs at a single-cell level by Raman spectroscopy revealed that despite similar biochemical background, fine differences in the Raman spectra of BM-MSCs of each donor can be detected. After extensive principal component analysis (PCA) of Raman spectra, our study revealed the possibility of this method to diversify BM-MSCs populations, whereby the grouping of cell populations was most prominent when cell populations were analyzed in pairs. These results indicate that Raman spectroscopy, as a label-free assay, could have a huge potential in understanding stem cell heterogeneity and sorting cell populations with a similar biochemical background that can be significant for the development of personalized therapy approaches.
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spelling pubmed-91030702022-05-14 A Single-Cell Raman Spectroscopy Analysis of Bone Marrow Mesenchymal Stem/Stromal Cells to Identify Inter-Individual Diversity Kukolj, Tamara Lazarević, Jasmina Borojević, Ana Ralević, Uroš Vujić, Dragana Jauković, Aleksandra Lazarević, Nenad Bugarski, Diana Int J Mol Sci Article The heterogeneity of stem cells represents the main challenge in regenerative medicine development. This issue is particularly pronounced when it comes to the use of primary mesenchymal stem/stromal cells (MSCs) due to a lack of identification markers. Considering the need for additional approaches in MSCs characterization, we applied Raman spectroscopy to investigate inter-individual differences between bone marrow MSCs (BM-MSCs). Based on standard biological tests, BM-MSCs of analyzed donors fulfill all conditions for their characterization, while no donor-related specifics were observed in terms of BM-MSCs morphology, phenotype, multilineage differentiation potential, colony-forming capacity, expression of pluripotency-associated markers or proliferative capacity. However, examination of BM-MSCs at a single-cell level by Raman spectroscopy revealed that despite similar biochemical background, fine differences in the Raman spectra of BM-MSCs of each donor can be detected. After extensive principal component analysis (PCA) of Raman spectra, our study revealed the possibility of this method to diversify BM-MSCs populations, whereby the grouping of cell populations was most prominent when cell populations were analyzed in pairs. These results indicate that Raman spectroscopy, as a label-free assay, could have a huge potential in understanding stem cell heterogeneity and sorting cell populations with a similar biochemical background that can be significant for the development of personalized therapy approaches. MDPI 2022-04-28 /pmc/articles/PMC9103070/ /pubmed/35563306 http://dx.doi.org/10.3390/ijms23094915 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kukolj, Tamara
Lazarević, Jasmina
Borojević, Ana
Ralević, Uroš
Vujić, Dragana
Jauković, Aleksandra
Lazarević, Nenad
Bugarski, Diana
A Single-Cell Raman Spectroscopy Analysis of Bone Marrow Mesenchymal Stem/Stromal Cells to Identify Inter-Individual Diversity
title A Single-Cell Raman Spectroscopy Analysis of Bone Marrow Mesenchymal Stem/Stromal Cells to Identify Inter-Individual Diversity
title_full A Single-Cell Raman Spectroscopy Analysis of Bone Marrow Mesenchymal Stem/Stromal Cells to Identify Inter-Individual Diversity
title_fullStr A Single-Cell Raman Spectroscopy Analysis of Bone Marrow Mesenchymal Stem/Stromal Cells to Identify Inter-Individual Diversity
title_full_unstemmed A Single-Cell Raman Spectroscopy Analysis of Bone Marrow Mesenchymal Stem/Stromal Cells to Identify Inter-Individual Diversity
title_short A Single-Cell Raman Spectroscopy Analysis of Bone Marrow Mesenchymal Stem/Stromal Cells to Identify Inter-Individual Diversity
title_sort single-cell raman spectroscopy analysis of bone marrow mesenchymal stem/stromal cells to identify inter-individual diversity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9103070/
https://www.ncbi.nlm.nih.gov/pubmed/35563306
http://dx.doi.org/10.3390/ijms23094915
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