Activities of Antioxidant and Proteolytic Systems and Biomarkers in the Fat Body and Hemolymph of Young Apis mellifera Females

SIMPLE SUMMARY: The proteolytic system consists of compounds that, similar to “scissors”, cut proteins found in bee cells (e.g., to activate these proteins) or released by pathogens. During these reactions, reactive oxygen species are created and then removed by antioxidants. The actions of the proteolytic and antioxidant systems are enhanced by biomarkers. These compounds are produced mainly in the fat body and then released into the hemolymph. We determined the activities of these compounds in various localizations/segments of the fat body and in the hemolymph in females with increased reproductive potential, i.e., queens and rebels, and in normal (sterile non-rebel) workers. Rebels are workers who resemble the queen in terms of anatomical, behavioural, and physiological features. It was revealed that the activities of these compounds in the rebels were between those of queens and normal workers. Normal workers had higher activities of the proteolytic and antioxidant systems in the fat body and hemolymph than the other females. These results are important for understanding the functioning of the fat body, the stress ecology, and the formation of the different castes of Apis mellifera females. ABSTRACT: The proteolytic and antioxidant systems are important components of humoral immunity, and these biomarkers indicate the immune status. These compounds are synthesized in the bees’ fat body and released into the hemolymph. Their functions maintain the organism’s homeostasis and protect it against adverse environmental factors (including pathogens). We determined the activities of acidic, neutral, and alkaline proteases and their inhibitors, as well as superoxide dismutase (SOD), catalase (CAT), aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and the level of total antioxidant potential (TAC). These compounds were investigated in the fat body and hemolymph in the females with increased reproductive potential, i.e., queens and rebels, and in normal (non-reproductive sterile non-rebel) workers. The phenoloxidase (PO) activities were determined in the hemolymph. The normal workers had higher activities of proteases and their inhibitors, SOD and CAT, in the fat body and hemolymph, compared to the queens and rebels. The protease inhibitors were not usually active in the queens. As we predicted, the rebels revealed values between those of the queens and normal workers. The highest activities of proteases and antioxidants were identified in the fat body from the third tergite in comparison with the sternite and the fifth tergite. These results are important for oxidative stress ecology and give a better understanding of the functioning of the fat body and the division of labor in social insects.