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Novasomes as Nano-Vesicular Carriers to Enhance Topical Delivery of Fluconazole: A New Approach to Treat Fungal Infections

The occurrence of fungal infections has increased over the past two decades. It is observed that superficial fungal infections are treated by conventional dosage forms, which are incapable of treating deep infections due to the barrier activity possessed by the stratum corneum of the skin. This is w...

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Autores principales: Fatima, Iman, Rasul, Akhtar, Shah, Shahid, Saadullah, Malik, Islam, Nayyer, Khames, Ahmed, Salawi, Ahmad, Ahmed, Muhammad Masood, Almoshari, Yosif, Abbas, Ghulam, Abourehab, Mohammed A. S., Mehmood Khan, Sajid, Chauhdary, Zunera, Alshamrani, Meshal, Namazi, Nader Ibrahim, Naguib, Demiana M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9103678/
https://www.ncbi.nlm.nih.gov/pubmed/35566287
http://dx.doi.org/10.3390/molecules27092936
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author Fatima, Iman
Rasul, Akhtar
Shah, Shahid
Saadullah, Malik
Islam, Nayyer
Khames, Ahmed
Salawi, Ahmad
Ahmed, Muhammad Masood
Almoshari, Yosif
Abbas, Ghulam
Abourehab, Mohammed A. S.
Mehmood Khan, Sajid
Chauhdary, Zunera
Alshamrani, Meshal
Namazi, Nader Ibrahim
Naguib, Demiana M.
author_facet Fatima, Iman
Rasul, Akhtar
Shah, Shahid
Saadullah, Malik
Islam, Nayyer
Khames, Ahmed
Salawi, Ahmad
Ahmed, Muhammad Masood
Almoshari, Yosif
Abbas, Ghulam
Abourehab, Mohammed A. S.
Mehmood Khan, Sajid
Chauhdary, Zunera
Alshamrani, Meshal
Namazi, Nader Ibrahim
Naguib, Demiana M.
author_sort Fatima, Iman
collection PubMed
description The occurrence of fungal infections has increased over the past two decades. It is observed that superficial fungal infections are treated by conventional dosage forms, which are incapable of treating deep infections due to the barrier activity possessed by the stratum corneum of the skin. This is why the need for a topical preparation with advanced penetration techniques has arisen. This research aimed to encapsulate fluconazole (FLZ) in a novasome in order to improve the topical delivery. The novasomes were prepared using the ethanol injection technique and characterized for percent entrapment efficiency (EE), particle size (PS), zeta potential (ZP), drug release, Fourier-transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), scanning electron microscopy (SEM) and antifungal activity. The FN7 formulation with 94.45% EE, 110 nm PS and −24 ZP proved to be the best formulation. The FN7 formulation showed a 96% release of FLZ in 8 h. FTIR showed the compatibility of FLZ with excipients and DSC studies confirmed the thermal stability of FLZ in the developed formulation. The FN7 formulation showed superior inhibition of the growth of Candida albicans compared to the FLZ suspension using a resazurin reduction assay, suggesting high efficacy in inhibiting fungal growth.
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spelling pubmed-91036782022-05-14 Novasomes as Nano-Vesicular Carriers to Enhance Topical Delivery of Fluconazole: A New Approach to Treat Fungal Infections Fatima, Iman Rasul, Akhtar Shah, Shahid Saadullah, Malik Islam, Nayyer Khames, Ahmed Salawi, Ahmad Ahmed, Muhammad Masood Almoshari, Yosif Abbas, Ghulam Abourehab, Mohammed A. S. Mehmood Khan, Sajid Chauhdary, Zunera Alshamrani, Meshal Namazi, Nader Ibrahim Naguib, Demiana M. Molecules Article The occurrence of fungal infections has increased over the past two decades. It is observed that superficial fungal infections are treated by conventional dosage forms, which are incapable of treating deep infections due to the barrier activity possessed by the stratum corneum of the skin. This is why the need for a topical preparation with advanced penetration techniques has arisen. This research aimed to encapsulate fluconazole (FLZ) in a novasome in order to improve the topical delivery. The novasomes were prepared using the ethanol injection technique and characterized for percent entrapment efficiency (EE), particle size (PS), zeta potential (ZP), drug release, Fourier-transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), scanning electron microscopy (SEM) and antifungal activity. The FN7 formulation with 94.45% EE, 110 nm PS and −24 ZP proved to be the best formulation. The FN7 formulation showed a 96% release of FLZ in 8 h. FTIR showed the compatibility of FLZ with excipients and DSC studies confirmed the thermal stability of FLZ in the developed formulation. The FN7 formulation showed superior inhibition of the growth of Candida albicans compared to the FLZ suspension using a resazurin reduction assay, suggesting high efficacy in inhibiting fungal growth. MDPI 2022-05-04 /pmc/articles/PMC9103678/ /pubmed/35566287 http://dx.doi.org/10.3390/molecules27092936 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fatima, Iman
Rasul, Akhtar
Shah, Shahid
Saadullah, Malik
Islam, Nayyer
Khames, Ahmed
Salawi, Ahmad
Ahmed, Muhammad Masood
Almoshari, Yosif
Abbas, Ghulam
Abourehab, Mohammed A. S.
Mehmood Khan, Sajid
Chauhdary, Zunera
Alshamrani, Meshal
Namazi, Nader Ibrahim
Naguib, Demiana M.
Novasomes as Nano-Vesicular Carriers to Enhance Topical Delivery of Fluconazole: A New Approach to Treat Fungal Infections
title Novasomes as Nano-Vesicular Carriers to Enhance Topical Delivery of Fluconazole: A New Approach to Treat Fungal Infections
title_full Novasomes as Nano-Vesicular Carriers to Enhance Topical Delivery of Fluconazole: A New Approach to Treat Fungal Infections
title_fullStr Novasomes as Nano-Vesicular Carriers to Enhance Topical Delivery of Fluconazole: A New Approach to Treat Fungal Infections
title_full_unstemmed Novasomes as Nano-Vesicular Carriers to Enhance Topical Delivery of Fluconazole: A New Approach to Treat Fungal Infections
title_short Novasomes as Nano-Vesicular Carriers to Enhance Topical Delivery of Fluconazole: A New Approach to Treat Fungal Infections
title_sort novasomes as nano-vesicular carriers to enhance topical delivery of fluconazole: a new approach to treat fungal infections
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9103678/
https://www.ncbi.nlm.nih.gov/pubmed/35566287
http://dx.doi.org/10.3390/molecules27092936
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