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Myoglobin Interaction with Lactate Rapidly Releases Oxygen: Studies on Binding Thermodynamics, Spectroscopy, and Oxygen Kinetics

Myoglobin (Mb)-mediated oxygen (O(2)) delivery and dissolved O(2) in the cytosol are two major sources that support oxidative phosphorylation. During intense exercise, lactate (LAC) production is elevated in skeletal muscles as a consequence of insufficient intracellular O(2) supply. The latter resu...

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Autores principales: Adepu, Kiran Kumar, Bhandari, Dipendra, Anishkin, Andriy, Adams, Sean H., Chintapalli, Sree V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9103699/
https://www.ncbi.nlm.nih.gov/pubmed/35563138
http://dx.doi.org/10.3390/ijms23094747
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author Adepu, Kiran Kumar
Bhandari, Dipendra
Anishkin, Andriy
Adams, Sean H.
Chintapalli, Sree V.
author_facet Adepu, Kiran Kumar
Bhandari, Dipendra
Anishkin, Andriy
Adams, Sean H.
Chintapalli, Sree V.
author_sort Adepu, Kiran Kumar
collection PubMed
description Myoglobin (Mb)-mediated oxygen (O(2)) delivery and dissolved O(2) in the cytosol are two major sources that support oxidative phosphorylation. During intense exercise, lactate (LAC) production is elevated in skeletal muscles as a consequence of insufficient intracellular O(2) supply. The latter results in diminished mitochondrial oxidative metabolism and an increased reliance on nonoxidative pathways to generate ATP. Whether or not metabolites from these pathways impact Mb-O(2) associations remains to be established. In the present study, we employed isothermal titration calorimetry, O(2) kinetic studies, and UV-Vis spectroscopy to evaluate the LAC affinity toward Mb (oxy- and deoxy-Mb) and the effect of LAC on O(2) release from oxy-Mb in varying pH conditions (pH 6.0–7.0). Our results show that LAC avidly binds to both oxy- and deoxy-Mb (only at acidic pH for the latter). Similarly, in the presence of LAC, increased release of O(2) from oxy-Mb was detected. This suggests that with LAC binding to Mb, the structural conformation of the protein (near the heme center) might be altered, which concomitantly triggers the release of O(2). Taken together, these novel findings support a mechanism where LAC acts as a regulator of O(2) management in Mb-rich tissues and/or influences the putative signaling roles for oxy- and deoxy-Mb, especially under conditions of LAC accumulation and lactic acidosis.
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spelling pubmed-91036992022-05-14 Myoglobin Interaction with Lactate Rapidly Releases Oxygen: Studies on Binding Thermodynamics, Spectroscopy, and Oxygen Kinetics Adepu, Kiran Kumar Bhandari, Dipendra Anishkin, Andriy Adams, Sean H. Chintapalli, Sree V. Int J Mol Sci Article Myoglobin (Mb)-mediated oxygen (O(2)) delivery and dissolved O(2) in the cytosol are two major sources that support oxidative phosphorylation. During intense exercise, lactate (LAC) production is elevated in skeletal muscles as a consequence of insufficient intracellular O(2) supply. The latter results in diminished mitochondrial oxidative metabolism and an increased reliance on nonoxidative pathways to generate ATP. Whether or not metabolites from these pathways impact Mb-O(2) associations remains to be established. In the present study, we employed isothermal titration calorimetry, O(2) kinetic studies, and UV-Vis spectroscopy to evaluate the LAC affinity toward Mb (oxy- and deoxy-Mb) and the effect of LAC on O(2) release from oxy-Mb in varying pH conditions (pH 6.0–7.0). Our results show that LAC avidly binds to both oxy- and deoxy-Mb (only at acidic pH for the latter). Similarly, in the presence of LAC, increased release of O(2) from oxy-Mb was detected. This suggests that with LAC binding to Mb, the structural conformation of the protein (near the heme center) might be altered, which concomitantly triggers the release of O(2). Taken together, these novel findings support a mechanism where LAC acts as a regulator of O(2) management in Mb-rich tissues and/or influences the putative signaling roles for oxy- and deoxy-Mb, especially under conditions of LAC accumulation and lactic acidosis. MDPI 2022-04-26 /pmc/articles/PMC9103699/ /pubmed/35563138 http://dx.doi.org/10.3390/ijms23094747 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Adepu, Kiran Kumar
Bhandari, Dipendra
Anishkin, Andriy
Adams, Sean H.
Chintapalli, Sree V.
Myoglobin Interaction with Lactate Rapidly Releases Oxygen: Studies on Binding Thermodynamics, Spectroscopy, and Oxygen Kinetics
title Myoglobin Interaction with Lactate Rapidly Releases Oxygen: Studies on Binding Thermodynamics, Spectroscopy, and Oxygen Kinetics
title_full Myoglobin Interaction with Lactate Rapidly Releases Oxygen: Studies on Binding Thermodynamics, Spectroscopy, and Oxygen Kinetics
title_fullStr Myoglobin Interaction with Lactate Rapidly Releases Oxygen: Studies on Binding Thermodynamics, Spectroscopy, and Oxygen Kinetics
title_full_unstemmed Myoglobin Interaction with Lactate Rapidly Releases Oxygen: Studies on Binding Thermodynamics, Spectroscopy, and Oxygen Kinetics
title_short Myoglobin Interaction with Lactate Rapidly Releases Oxygen: Studies on Binding Thermodynamics, Spectroscopy, and Oxygen Kinetics
title_sort myoglobin interaction with lactate rapidly releases oxygen: studies on binding thermodynamics, spectroscopy, and oxygen kinetics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9103699/
https://www.ncbi.nlm.nih.gov/pubmed/35563138
http://dx.doi.org/10.3390/ijms23094747
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