Cargando…
Combination of Hypotonic Lysis and Application of Detergent for Isolation of Polyhydroxyalkanoates from Extremophiles
Production of polyhydroxyalkanoates (PHA), microbial biopolyesters, employing extremophilic microorganisms is a very promising concept relying on robustness of such organisms against microbial contamination, which provides numerous economic and technological benefits. In this work, we took advantage...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9104112/ https://www.ncbi.nlm.nih.gov/pubmed/35566928 http://dx.doi.org/10.3390/polym14091761 |
_version_ | 1784707715274113024 |
---|---|
author | Novackova, Ivana Kourilova, Xenie Mrazova, Katerina Sedlacek, Petr Kalina, Michal Krzyzanek, Vladislav Koller, Martin Obruca, Stanislav |
author_facet | Novackova, Ivana Kourilova, Xenie Mrazova, Katerina Sedlacek, Petr Kalina, Michal Krzyzanek, Vladislav Koller, Martin Obruca, Stanislav |
author_sort | Novackova, Ivana |
collection | PubMed |
description | Production of polyhydroxyalkanoates (PHA), microbial biopolyesters, employing extremophilic microorganisms is a very promising concept relying on robustness of such organisms against microbial contamination, which provides numerous economic and technological benefits. In this work, we took advantage of the natural susceptibility of halophilic and thermophilic PHA producers to hypotonic lysis and we developed a simple and robust approach enabling effective isolation of PHA materials from microbial cells. The method is based on the exposition of microbial cells to hypotonic conditions induced by the diluted solution of sodium dodecyl sulfate (SDS) at elevated temperatures. Such conditions lead to disruption of the cells and release of PHA granules. Moreover, SDS, apart from its cell-disruptive function, also solubilizes hydrophobic components, which would otherwise contaminate PHA materials. The purity of obtained materials, as well as the yields of recovery, reach high values (values of purity higher than 99 wt.%, yields close to 1). Furthermore, we also focused on the removal of SDS from wastewater. The simple, inexpensive, and safe technique is based on the precipitation of SDS in the presence of KCl. The precipitate can be simply removed by decantation or centrifugation. Moreover, there is also the possibility to regenerate the SDS, which would substantially improve the economic feasibility of the process. |
format | Online Article Text |
id | pubmed-9104112 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91041122022-05-14 Combination of Hypotonic Lysis and Application of Detergent for Isolation of Polyhydroxyalkanoates from Extremophiles Novackova, Ivana Kourilova, Xenie Mrazova, Katerina Sedlacek, Petr Kalina, Michal Krzyzanek, Vladislav Koller, Martin Obruca, Stanislav Polymers (Basel) Article Production of polyhydroxyalkanoates (PHA), microbial biopolyesters, employing extremophilic microorganisms is a very promising concept relying on robustness of such organisms against microbial contamination, which provides numerous economic and technological benefits. In this work, we took advantage of the natural susceptibility of halophilic and thermophilic PHA producers to hypotonic lysis and we developed a simple and robust approach enabling effective isolation of PHA materials from microbial cells. The method is based on the exposition of microbial cells to hypotonic conditions induced by the diluted solution of sodium dodecyl sulfate (SDS) at elevated temperatures. Such conditions lead to disruption of the cells and release of PHA granules. Moreover, SDS, apart from its cell-disruptive function, also solubilizes hydrophobic components, which would otherwise contaminate PHA materials. The purity of obtained materials, as well as the yields of recovery, reach high values (values of purity higher than 99 wt.%, yields close to 1). Furthermore, we also focused on the removal of SDS from wastewater. The simple, inexpensive, and safe technique is based on the precipitation of SDS in the presence of KCl. The precipitate can be simply removed by decantation or centrifugation. Moreover, there is also the possibility to regenerate the SDS, which would substantially improve the economic feasibility of the process. MDPI 2022-04-26 /pmc/articles/PMC9104112/ /pubmed/35566928 http://dx.doi.org/10.3390/polym14091761 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Novackova, Ivana Kourilova, Xenie Mrazova, Katerina Sedlacek, Petr Kalina, Michal Krzyzanek, Vladislav Koller, Martin Obruca, Stanislav Combination of Hypotonic Lysis and Application of Detergent for Isolation of Polyhydroxyalkanoates from Extremophiles |
title | Combination of Hypotonic Lysis and Application of Detergent for Isolation of Polyhydroxyalkanoates from Extremophiles |
title_full | Combination of Hypotonic Lysis and Application of Detergent for Isolation of Polyhydroxyalkanoates from Extremophiles |
title_fullStr | Combination of Hypotonic Lysis and Application of Detergent for Isolation of Polyhydroxyalkanoates from Extremophiles |
title_full_unstemmed | Combination of Hypotonic Lysis and Application of Detergent for Isolation of Polyhydroxyalkanoates from Extremophiles |
title_short | Combination of Hypotonic Lysis and Application of Detergent for Isolation of Polyhydroxyalkanoates from Extremophiles |
title_sort | combination of hypotonic lysis and application of detergent for isolation of polyhydroxyalkanoates from extremophiles |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9104112/ https://www.ncbi.nlm.nih.gov/pubmed/35566928 http://dx.doi.org/10.3390/polym14091761 |
work_keys_str_mv | AT novackovaivana combinationofhypotoniclysisandapplicationofdetergentforisolationofpolyhydroxyalkanoatesfromextremophiles AT kourilovaxenie combinationofhypotoniclysisandapplicationofdetergentforisolationofpolyhydroxyalkanoatesfromextremophiles AT mrazovakaterina combinationofhypotoniclysisandapplicationofdetergentforisolationofpolyhydroxyalkanoatesfromextremophiles AT sedlacekpetr combinationofhypotoniclysisandapplicationofdetergentforisolationofpolyhydroxyalkanoatesfromextremophiles AT kalinamichal combinationofhypotoniclysisandapplicationofdetergentforisolationofpolyhydroxyalkanoatesfromextremophiles AT krzyzanekvladislav combinationofhypotoniclysisandapplicationofdetergentforisolationofpolyhydroxyalkanoatesfromextremophiles AT kollermartin combinationofhypotoniclysisandapplicationofdetergentforisolationofpolyhydroxyalkanoatesfromextremophiles AT obrucastanislav combinationofhypotoniclysisandapplicationofdetergentforisolationofpolyhydroxyalkanoatesfromextremophiles |