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Combination of Hypotonic Lysis and Application of Detergent for Isolation of Polyhydroxyalkanoates from Extremophiles

Production of polyhydroxyalkanoates (PHA), microbial biopolyesters, employing extremophilic microorganisms is a very promising concept relying on robustness of such organisms against microbial contamination, which provides numerous economic and technological benefits. In this work, we took advantage...

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Autores principales: Novackova, Ivana, Kourilova, Xenie, Mrazova, Katerina, Sedlacek, Petr, Kalina, Michal, Krzyzanek, Vladislav, Koller, Martin, Obruca, Stanislav
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9104112/
https://www.ncbi.nlm.nih.gov/pubmed/35566928
http://dx.doi.org/10.3390/polym14091761
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author Novackova, Ivana
Kourilova, Xenie
Mrazova, Katerina
Sedlacek, Petr
Kalina, Michal
Krzyzanek, Vladislav
Koller, Martin
Obruca, Stanislav
author_facet Novackova, Ivana
Kourilova, Xenie
Mrazova, Katerina
Sedlacek, Petr
Kalina, Michal
Krzyzanek, Vladislav
Koller, Martin
Obruca, Stanislav
author_sort Novackova, Ivana
collection PubMed
description Production of polyhydroxyalkanoates (PHA), microbial biopolyesters, employing extremophilic microorganisms is a very promising concept relying on robustness of such organisms against microbial contamination, which provides numerous economic and technological benefits. In this work, we took advantage of the natural susceptibility of halophilic and thermophilic PHA producers to hypotonic lysis and we developed a simple and robust approach enabling effective isolation of PHA materials from microbial cells. The method is based on the exposition of microbial cells to hypotonic conditions induced by the diluted solution of sodium dodecyl sulfate (SDS) at elevated temperatures. Such conditions lead to disruption of the cells and release of PHA granules. Moreover, SDS, apart from its cell-disruptive function, also solubilizes hydrophobic components, which would otherwise contaminate PHA materials. The purity of obtained materials, as well as the yields of recovery, reach high values (values of purity higher than 99 wt.%, yields close to 1). Furthermore, we also focused on the removal of SDS from wastewater. The simple, inexpensive, and safe technique is based on the precipitation of SDS in the presence of KCl. The precipitate can be simply removed by decantation or centrifugation. Moreover, there is also the possibility to regenerate the SDS, which would substantially improve the economic feasibility of the process.
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spelling pubmed-91041122022-05-14 Combination of Hypotonic Lysis and Application of Detergent for Isolation of Polyhydroxyalkanoates from Extremophiles Novackova, Ivana Kourilova, Xenie Mrazova, Katerina Sedlacek, Petr Kalina, Michal Krzyzanek, Vladislav Koller, Martin Obruca, Stanislav Polymers (Basel) Article Production of polyhydroxyalkanoates (PHA), microbial biopolyesters, employing extremophilic microorganisms is a very promising concept relying on robustness of such organisms against microbial contamination, which provides numerous economic and technological benefits. In this work, we took advantage of the natural susceptibility of halophilic and thermophilic PHA producers to hypotonic lysis and we developed a simple and robust approach enabling effective isolation of PHA materials from microbial cells. The method is based on the exposition of microbial cells to hypotonic conditions induced by the diluted solution of sodium dodecyl sulfate (SDS) at elevated temperatures. Such conditions lead to disruption of the cells and release of PHA granules. Moreover, SDS, apart from its cell-disruptive function, also solubilizes hydrophobic components, which would otherwise contaminate PHA materials. The purity of obtained materials, as well as the yields of recovery, reach high values (values of purity higher than 99 wt.%, yields close to 1). Furthermore, we also focused on the removal of SDS from wastewater. The simple, inexpensive, and safe technique is based on the precipitation of SDS in the presence of KCl. The precipitate can be simply removed by decantation or centrifugation. Moreover, there is also the possibility to regenerate the SDS, which would substantially improve the economic feasibility of the process. MDPI 2022-04-26 /pmc/articles/PMC9104112/ /pubmed/35566928 http://dx.doi.org/10.3390/polym14091761 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Novackova, Ivana
Kourilova, Xenie
Mrazova, Katerina
Sedlacek, Petr
Kalina, Michal
Krzyzanek, Vladislav
Koller, Martin
Obruca, Stanislav
Combination of Hypotonic Lysis and Application of Detergent for Isolation of Polyhydroxyalkanoates from Extremophiles
title Combination of Hypotonic Lysis and Application of Detergent for Isolation of Polyhydroxyalkanoates from Extremophiles
title_full Combination of Hypotonic Lysis and Application of Detergent for Isolation of Polyhydroxyalkanoates from Extremophiles
title_fullStr Combination of Hypotonic Lysis and Application of Detergent for Isolation of Polyhydroxyalkanoates from Extremophiles
title_full_unstemmed Combination of Hypotonic Lysis and Application of Detergent for Isolation of Polyhydroxyalkanoates from Extremophiles
title_short Combination of Hypotonic Lysis and Application of Detergent for Isolation of Polyhydroxyalkanoates from Extremophiles
title_sort combination of hypotonic lysis and application of detergent for isolation of polyhydroxyalkanoates from extremophiles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9104112/
https://www.ncbi.nlm.nih.gov/pubmed/35566928
http://dx.doi.org/10.3390/polym14091761
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