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Micro-RNAs in Response to Active Forms of Vitamin D(3) in Human Leukemia and Lymphoma Cells

Non-coding micro-RNA (miRNAs) regulate the protein expression responsible for cell growth and proliferation. miRNAs also play a role in a cancer cells’ response to drug treatment. Knowing that leukemia and lymphoma cells show different responses to active forms of vitamin D(3), we decided to investi...

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Autores principales: Gleba, Justyna Joanna, Kłopotowska, Dagmara, Banach, Joanna, Mielko, Karolina Anna, Turlej, Eliza, Maciejewska, Magdalena, Kutner, Andrzej, Wietrzyk, Joanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9104187/
https://www.ncbi.nlm.nih.gov/pubmed/35563410
http://dx.doi.org/10.3390/ijms23095019
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author Gleba, Justyna Joanna
Kłopotowska, Dagmara
Banach, Joanna
Mielko, Karolina Anna
Turlej, Eliza
Maciejewska, Magdalena
Kutner, Andrzej
Wietrzyk, Joanna
author_facet Gleba, Justyna Joanna
Kłopotowska, Dagmara
Banach, Joanna
Mielko, Karolina Anna
Turlej, Eliza
Maciejewska, Magdalena
Kutner, Andrzej
Wietrzyk, Joanna
author_sort Gleba, Justyna Joanna
collection PubMed
description Non-coding micro-RNA (miRNAs) regulate the protein expression responsible for cell growth and proliferation. miRNAs also play a role in a cancer cells’ response to drug treatment. Knowing that leukemia and lymphoma cells show different responses to active forms of vitamin D(3), we decided to investigate the role of selected miRNA molecules and regulated proteins, analyzing if there is a correlation between the selected miRNAs and regulated proteins in response to two active forms of vitamin D(3), calcitriol and tacalcitol. A total of nine human cell lines were analyzed: five leukemias: MV-4-1, Thp-1, HL-60, K562, and KG-1; and four lymphomas: Raji, Daudi, Jurkat, and U2932. We selected five miRNA molecules—miR-27b, miR-32, miR-125b, miR-181a, and miR-181b—and the proteins regulated by these molecules, namely, CYP24A1, Bak1, Bim, p21, p27, p53, and NF-kB. The results showed that the level of selected miRNAs correlates with the level of proteins, especially p27, Bak1, NFκB, and CYP24A1, and miR-27b and miR-125b could be responsible for the anticancer activity of active forms of vitamin D(3) in human leukemia and lymphoma.
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spelling pubmed-91041872022-05-14 Micro-RNAs in Response to Active Forms of Vitamin D(3) in Human Leukemia and Lymphoma Cells Gleba, Justyna Joanna Kłopotowska, Dagmara Banach, Joanna Mielko, Karolina Anna Turlej, Eliza Maciejewska, Magdalena Kutner, Andrzej Wietrzyk, Joanna Int J Mol Sci Article Non-coding micro-RNA (miRNAs) regulate the protein expression responsible for cell growth and proliferation. miRNAs also play a role in a cancer cells’ response to drug treatment. Knowing that leukemia and lymphoma cells show different responses to active forms of vitamin D(3), we decided to investigate the role of selected miRNA molecules and regulated proteins, analyzing if there is a correlation between the selected miRNAs and regulated proteins in response to two active forms of vitamin D(3), calcitriol and tacalcitol. A total of nine human cell lines were analyzed: five leukemias: MV-4-1, Thp-1, HL-60, K562, and KG-1; and four lymphomas: Raji, Daudi, Jurkat, and U2932. We selected five miRNA molecules—miR-27b, miR-32, miR-125b, miR-181a, and miR-181b—and the proteins regulated by these molecules, namely, CYP24A1, Bak1, Bim, p21, p27, p53, and NF-kB. The results showed that the level of selected miRNAs correlates with the level of proteins, especially p27, Bak1, NFκB, and CYP24A1, and miR-27b and miR-125b could be responsible for the anticancer activity of active forms of vitamin D(3) in human leukemia and lymphoma. MDPI 2022-04-30 /pmc/articles/PMC9104187/ /pubmed/35563410 http://dx.doi.org/10.3390/ijms23095019 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gleba, Justyna Joanna
Kłopotowska, Dagmara
Banach, Joanna
Mielko, Karolina Anna
Turlej, Eliza
Maciejewska, Magdalena
Kutner, Andrzej
Wietrzyk, Joanna
Micro-RNAs in Response to Active Forms of Vitamin D(3) in Human Leukemia and Lymphoma Cells
title Micro-RNAs in Response to Active Forms of Vitamin D(3) in Human Leukemia and Lymphoma Cells
title_full Micro-RNAs in Response to Active Forms of Vitamin D(3) in Human Leukemia and Lymphoma Cells
title_fullStr Micro-RNAs in Response to Active Forms of Vitamin D(3) in Human Leukemia and Lymphoma Cells
title_full_unstemmed Micro-RNAs in Response to Active Forms of Vitamin D(3) in Human Leukemia and Lymphoma Cells
title_short Micro-RNAs in Response to Active Forms of Vitamin D(3) in Human Leukemia and Lymphoma Cells
title_sort micro-rnas in response to active forms of vitamin d(3) in human leukemia and lymphoma cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9104187/
https://www.ncbi.nlm.nih.gov/pubmed/35563410
http://dx.doi.org/10.3390/ijms23095019
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